Tonsil-derived mesenchymal stem cells exert immunosuppressive effects on T cells

Abstract

Aim To assess the immunomodulatory effect of tonsil-derived mesenchymal stem cells (MSCs) on T-lymphocyte proliferation and cytokine production. Methods Tonsils were obtained from children aged 3 to 12 years (n = 15) who underwent tonsillectomy for obstructive sleep apnea from April 2012-October 2014 at the Merkur University Hospital, Zagreb. Tonsil-derived MSCs were co-cultured with peripheral blood mononuclear cells (PBMCs) and phytohemagglutinin as a mitogen. PBMCs were induced to differentiate into T helper 1 or T helper 2 cells in the presence or absence of tonsil-derived MSCs, after which the production of interferon-gamma in T helper 1 and interleukin-4 in T helper 2 cells was assessed. Results Tonsil-derived MSC suppressed phytohemagglutinin-induced proliferation of PBMCs. Compared with controls, tonsil-derived MSC co-culture significantly decreased interferon-gamma production ( P < 0.001) and increased interleukin-4 production ( P < 0.001). Conclusion Tonsil-derived MSCs exert immunomodulatory effects on T lymphocyte proliferation and T helper 1- and T helper 2-specific cytokine production.

Alteration of payload in extracellular vesicles by crosstalk with mesenchymal stem cells from different origin

“…MSCs were extracted from three different origin (Adipose tissue, Bone marrow, and Tonsil), and the change in payload of EVs obtained by co-culture with middle ear epithelial cells (HMEEC) in transwells was analyzed. Adipose tissue (AD)-derived MSCs express mRNAs related to transcription factors, angiogenesis, and adipogenesis [18,27]. Bone marrow (BM)-derived MSCs are widely known for their regenerative, immune stimulatory, and related factors, such as calcium signaling and cytoskeletal genes [20].…”

“…Tonsil-derived MSCs were extracted as described by Bacic et al [27]. The tonsil tissue was gently washed with ethanol and cut with surgical scissors on a plate.…”

Alteration of Payload in Extracellular Vesicles by Crosstalk With Mesenchymal Stem Cells From Different Origin

“…Upon reaching 70% con uence, the cells were passaged to 1 × 10 6 cells/plate. Tonsil-derived MSCs were extracted as described by Bacic et al [27]. The tonsil tissue was gently washed with ethanol and cut with surgical scissors on a plate.…”

Role of Mesenchymal Stem Cells on Tonsillar Hypertrophy: An Unexplored Enigma

“…There are studies suggesting the presence of mesenchymal stem cells in the tonsils. 14 Palatine tonsil is a good source of MSC. The tonsillar epithelium is originated from the endoderm and develops through the second pharyngeal pouch.…”