Alteration of payload in extracellular vesicles by crosstalk with mesenchymal stem cells from different origin

Park, Jeong-Eun; Kong, Tae Hoon; Seo, Young Joon

doi:10.1186/s12951-021-00890-9

Cited by 6 publications

(4 citation statements)

References 70 publications

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“…The schematic of this diagram is to present an approach direction of exosome-based therapy (exo-therapy) for application to hearing loss (Figure 3). Stem cell derived exosomes are used for communication between cell to cell, and it is known that the payload changed depending on the origin of the stem cells [55]. It has been reported that miRNAs and proteins generated in communication between donor cells and target cells are different.…”

Section: Future Prospectsmentioning

confidence: 99%

“…It has been reported that miRNAs and proteins generated in communication between donor cells and target cells are different. In other words, the author believe that it may be helpful to selectively deliver interest genes to cure tissue or cells in destination using exosome with different payload [55]. The approach direction of exosome-based therapy (exo-therapy) for applying to hearing loss was described and its advantages were introduced.…”

Section: Future Prospectsmentioning

confidence: 99%

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Standardized Methodologies to Utilize Exosome Treatment as Potential Nano Substances in Hearing Loss

Park

2021

JOHBM

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Recently, studies on the mechanism and clinical application of stem cell-derived exosomes have increased. Although the number of patients with hearing loss is increasing, there is no ideal therapy for the recovery of auditory cells of an independent organ in humans. In this review, we proposed the use of stem cell-derived exosomes for treating hearing loss and summarized the exosome research strategy platform for preclinical studies. It is necessary to select a research direction to assess direct or indirect effects on recipients based on the physiological mechanisms of exosomes that deliver useful molecules (called payloads) to recipient cells or tissues. To apply exosomes in the auditory field, researchers should select a model for assessing the toxicity to the auditory cells and analyzing their mechanisms in the recipient tissue. Such in vitro, ex vivo, and in vivo models have been designed and reported in previous studies. The analytical strategies in various models can evaluate the mechanism of exosomes based on exosome surface markers or the payload, thus helping the researchers in finding evidence regarding the efficacy of exosomes. Here, we propose three strategies for exosome application research in the auditory field.

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Section: Future Prospectsmentioning

confidence: 99%

Section: Future Prospectsmentioning

confidence: 99%

Standardized Methodologies to Utilize Exosome Treatment as Potential Nano Substances in Hearing Loss

Park

2021

JOHBM

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“…Stem cells, both tendon stem/progenitor cells (TSPCs) and mesenchymal stem cells (MSCs) from other tissues, have been extensively studied in tendon healing and tendinopathy treatments [ 5 , 6 ]. Common sources of MSCs include bone marrow, fat, gums, and dental pulp; thus, it is natural that biological diversities exist among EVs derived from different MSCs [ 7 ]. Adipose tissue-derived mesenchymal stem cells (ADMSCs) demonstrate tissue repair potentials through differentiate into target cells in the injured area, secretion of various cytokines/growth factors to neighboring cells as well as immunomodulatory effects to reduce the inflammatory response in damaged tissues.…”

Section: Introductionmentioning

confidence: 99%

NAMPT encapsulated by extracellular vesicles from young adipose-derived mesenchymal stem cells treated tendinopathy in a “One-Stone-Two-Birds” manner

et al. 2023

J Nanobiotechnol

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Background Tendinopathy is the leading sports-related injury and will cause severe weakness and tenderness. Effective therapy for tendinopathy remains limited, and extracellular vesicles (EVs) derived from adipose tissue-derived mesenchymal stem cells (ADMSCs) have demonstrated great potential in tendinopathy treatment; however, the influence of aging status on EV treatment has not been previously described. Results In this study, it was found that ADMSCs derived from old mice (ADMSCold) demonstrated remarkable cellular senescence and impaired NAD+ metabolism compared with ADMSCs derived from young mice (ADMSCyoung). Lower NAMPT contents were detected in both ADMSCold and its secreted EVs (ADMSCold-EVs). Advanced animal experiments demonstrated that ADMSCyoung-EVs, but not ADMSCold-EVs, alleviated the pathological structural, functional and biomechanical properties in tendinopathy mice. Mechanistic analyses demonstrated that ADMSCyoung-EVs improved cell viability and relieved cellular senescence of tenocytes through the NAMPT/SIRT1/PPARγ/PGC-1α pathway. ADMSCyoung-EVs, but not ADMSCold-EVs, promoted phagocytosis and M2 polarization in macrophages through the NAMPT/SIRT1/Nf-κb p65/NLRP3 pathway. The macrophage/tenocyte crosstalk in tendinopathy was influenced by ADMSCyoung-EV treatment and thus it demonstrated "One-Stone-Two-Birds" effects in tendinopathy treatment. Conclusions This study demonstrates an effective novel therapy for tendinopathy and uncovers the influence of donor age on curative effects by clarifying the detailed biological mechanism. Graphical Abstract

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“…Given the similar properties, cellderived biomimetic nanovesicles could be utilized for drug delivery [24,25], tissue regeneration [26,27], and cancer targeting [28,29]. In particular, it was reported that nanovesicles derived from human tonsil-derived mesenchymal stem cells attenuate liver fibrosis and inflammation [30,31]. However, the anti-aging effects of nanovesicles derived from human tonsil-derived mesenchymal stem cells (TMSCs) have not yet been investigated.…”

Section: Introductionmentioning

confidence: 99%

Anti-Aging Effects of Nanovesicles Derived from Human Tonsil-Derived Mesenchymal Stem Cells

Kim¹,

Lee

Park

et al. 2021

Applied Sciences

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Growing evidence has demonstrated that biomimetic nanovesicles produced from specific cells show bioactive properties such as anti-tumor or anti-inflammatory activities. However, the properties of these nanovesicles are very diverse, depending on their cell sources. In this study, human tonsil-derived mesenchymal stem cells (TMSCs) were used in the production of functional biomimetic nanovesicles with anti-senescence. TMSCs were isolated from human tonsil tissue obtained by tonsillectomy. TMSC-derived nanovesicles (TMSC-NVs) were produced by serial extrusion using a mini-extruder. Western blotting and particle analysis were performed for characterization of TMSC-NVs. They were applied to both replicative and ultraviolet B-induced senescent human dermal fibroblasts in vitro. Following six days of treatment, analysis of the proliferation and senescence level of fibroblasts was performed using cell counting and senescence-associated β-galactosidase assay, respectively. Treatment with TMSC-NVs enhanced the cell proliferation and reduced the activity of senescence-associated β-galactosidase in both replicative and ultraviolet B-induced senescent cells. Treatment with TMSC-NVs resulted in increased expression of extracellular matrix and anti-oxidant genes. Treatment with TMSC-NVs resulted in reduced expression of vinculin in focal adhesion. These results show that TMSC-NVs have an effect on recovering from cellular senescence by oxidative stress and can be applied as useful materials for the development of skin rejuvenation.

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Alteration of payload in extracellular vesicles by crosstalk with mesenchymal stem cells from different origin

Cited by 6 publications

References 70 publications

Standardized Methodologies to Utilize Exosome Treatment as Potential Nano Substances in Hearing Loss

Standardized Methodologies to Utilize Exosome Treatment as Potential Nano Substances in Hearing Loss

NAMPT encapsulated by extracellular vesicles from young adipose-derived mesenchymal stem cells treated tendinopathy in a “One-Stone-Two-Birds” manner

Anti-Aging Effects of Nanovesicles Derived from Human Tonsil-Derived Mesenchymal Stem Cells

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