Tongue Microbiota and Oral Health Status in Community-Dwelling Elderly Adults

Asakawa, Mikari; Takeshita, Toru; Furuta, Michiko; Kageyama, Shinya; Takeuchi, Kenji; Hata, Jun; Ninomiya, Toshiharu; Yamashita, Yoshihisa

doi:10.1128/msphere.00332-18

Cited by 83 publications

(91 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The tongue coating of community-dwelling adults was collected using a sampling device based on an electric toothbrush developed in our previous study [26], which enabled us to collect bacteria attached to a bonded-fiber fabric on a brush head from a 15 mm-diameter circular area on the center of the tongue dorsum. On the other hand, we had already commenced with sample collection from allo-HSCT patients before development of this device, thus their samples were collected by using a conventional cotton swab.…”

Section: Plos Pathogensmentioning

confidence: 99%

“…This population consisted of 164 adult residents of Hisayama Town, Fukuoka, Japan who received a health examination as well as periodontal disease screenings for adults aged 40, 50, 60 and 70 years in 2016 (n = 41, 35, 53, and 35 subjects, respectively). The subjects aged 70 years were part of the study population of our previous study on the tongue microbiota of community-dwelling elderly adults aged 70 to 80 years [26]. Sample collection using a sampling device based on an electric toothbrush with a bonded-fiber fabric and DNA extraction were conducted as previously described [26].…”

Section: Plos Pathogensmentioning

confidence: 99%

“…The subjects aged 70 years were part of the study population of our previous study on the tongue microbiota of community-dwelling elderly adults aged 70 to 80 years [26]. Sample collection using a sampling device based on an electric toothbrush with a bonded-fiber fabric and DNA extraction were conducted as previously described [26]. The samples from the subjects aged 40, 50 and 60 years were collected in the same health examination and processed using the same procedure.…”

Section: Plos Pathogensmentioning

confidence: 99%

“…The V1-V2 region of 16S rRNA gene of the 35 community-dwelling adults aged 70 years was determined in our previous study [26] with the Ion PGM (Thermo Fisher Scientific) and the same reagents used in this study, except that the previous reverse primer did not contain an 8-base tag sequence. The V1-V2 regions of 16S rRNA gene sequences of the remaining 130 residents aged 40, 50 and 60 years were determined via the same procedure.…”

Section: Ion Torrent 16s Rrna Gene Sequencing Analysismentioning

confidence: 99%

“…The quality of the raw sequence reads was checked using a script written in R as previously described [26]. The quality-filtered reads were assigned to the appropriate samples based on their tag sequences, followed by trimming their adaptor, tag, and forward primer sequences.…”

Section: Ion Torrent 16s Rrna Gene Sequencing Analysismentioning

confidence: 99%

See 4 more Smart Citations

Disrupted tongue microbiota and detection of nonindigenous bacteria on the day of allogeneic hematopoietic stem cell transplantation

et al. 2020

Self Cite

View full text Add to dashboard Cite

Disruption of the intestinal microbiota caused by intensive chemotherapy, irradiation and antibiotics can result in development of severe gut graft-versus-host disease and infectious complications, leading to poorer outcomes among allogeneic hematopoietic stem cell transplantation (allo-HSCT) recipients. Although the oral cavity is also densely colonized by indigenous microorganisms, the bacterial composition in allo-HSCT recipients remains unclear. We determined the tongue microbiota composition of 45 patients with hematological disorders on the day of transplantation and compared them to 164 community-dwelling adults. The V1-V2 regions of the 16S rRNA gene sequences demonstrated that the allo-HSCT recipients had less diverse and distinct microbiota from that of community-dwelling adults. The full-length 16S rRNA gene sequences identified 146 bacterial taxa in the microbiota of allo-HSCT recipients, of which 34 bacterial taxa did not correspond to bacteria primarily inhabiting the oral cavity deposited in the expanded Human Oral Microbiome Database. Notably, the detection of Staphylococcus haemolyticus and/or Ralstonia pickettii was significantly associated with a higher risk of mortality during the follow-up period. These results demonstrate that the oral cavity of allo-HSCT recipients is colonized by a disrupted microbiota on the day of transplantation and suggest that detection of specific nonindigenous taxa could be a predictor of transplant outcome. PLOS PATHOGENSPLOS Pathogens | https://doi.org/10.Allogeneic hematopoietic stem cell transplantation (allo-HSCT) recipients are subjected to intensive chemotherapy, irradiation and antibiotics which could affect the intestinal as well as oral microbiota. We employed full-length 16S rRNA gene sequencing analysis with high taxonomic resolution using a third-generation sequencer, PacBio Sequel, and determined the bacterial composition of the tongue microbiota of allo-HSCT recipients after conditioning regimens. This comprehensive molecular approach identified 34 taxa uncommon in the oral cavity, which constituted 0-99.4% (median, 0.27%) of each tongue microbiota. Of them, Staphylococcus haemolyticus and Ralstonia pickettii were frequently found in allo-HSCT recipients, and their detection was significantly associated with a higher risk of mortality during the follow-up period. These results suggest that careful attention should be given to the bacterial composition of the disrupted oral microbiota in allo-HSCT recipients. PLOS PATHOGENSDisrupted oral microbiota of allo-HSCT recipients PLOS Pathogens | https://doi.org/10.

show abstract

Section: Plos Pathogensmentioning

confidence: 99%

Section: Plos Pathogensmentioning

confidence: 99%