TOMCAT: A beamline for TOmographic Microscopy and Coherent rAdiology experimenTs

Stampanoni, Marco; Groso, Amela; Isenegger, A.; Mikuljan, G.; Chen, Q.; Meister, D.; Lange, M.; Betemps, R.; Henein, Simon

doi:10.1063/1.2436193

Cited by 100 publications

(87 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The first two components have been previously described in detail elsewhere [61,62,84,85] and thus are only recapitulated briefly here. The em-phasis of the present work is on the third component; the experimental methods are described in detail below.…”

Section: Methodsmentioning

confidence: 99%

“…Thirty minutes after instillation, the lungs were fixed with 2.5 % glutaraldehyde by vascular perfusion, stained according to standard protocols for TEM and embedded in Epon. The samples were shaped to cylinders with a diameter of either 0.6 or 1.2 mm and a length of several millimeters using a watchmakers lathe and then scanned at TOMCAT [61], the beamline for TOmographic Microscopy and Coherent rAdiology experimenTs at the Swiss Light Source at the Paul Scherrer Institute in Villigen, Switzerland. The samples were scanned at a wavelength of 11.5 keV, post processed on a 20-node Linux cluster using filtered backprojection, resulting in an image stack of 2048×2048×2048 pixels with isotropic voxels of 350 nm side length.…”

Section: Methodsmentioning

confidence: 99%

“…In the past few years, however, µCT-based 3d reconstruction and morphometric analysis of the alveolar region has become possible [79,83,115], facilitated by the advent of Synchrotron Radiation Computed Tomography (SRCT). SRCT is to be distinguished from SRXTM [18,48,61]. While during SRCT the X-rays are directly recorded after transmitting the sample, during SRXTM the transmitted images are first recorded on a scintillator and magnified 5-20 times by a light microscope before recording.…”

Section: Historical Perspectivementioning

confidence: 99%

“…Light Source (SLS) of the Paul Scherrer Institut (Villigen, Switzerland) [48,61]. After penetration of the heavy metal stained sample, X-rays were converted into visible light by a thin Ce-doped YAG scintillator screen (Crismatec Saint-Gobain, Nemours, France).…”

Section: Image Acquisitionmentioning

confidence: 99%

See 3 more Smart Citations

High resolution tomographic imaging of the alveolar region of the mammalian lung

Haberthür¹

2010

SciVee

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Historical Perspectivementioning

confidence: 99%

Section: Image Acquisitionmentioning

confidence: 99%

See 2 more Smart Citations

High resolution tomographic imaging of the alveolar region of the mammalian lung

Haberthür¹

2010

SciVee

View full text Add to dashboard Cite

“…W-based multilayer systems, such as W/Si [8,9] and W/B 4 C [10,11] , are fabricated to produce ordinary monochromators with a spectral resolution of 1%-2%. Meanwhile, Al 2 O 3 /B 4 C [12] , SiC/C [13] , and even C/C [14] can be used for higher resolution.…”

mentioning

confidence: 99%

Small d-spacing WSi2/Si multilayers for X-ray monochromators

Huang

Zhu

et al. 2012

中国光学快报

View full text Add to dashboard Cite

A WSi2/Si multilayer, with 300 bi-layers and a 2.18-nm d-spacing, is designed for X-ray monochromator application. The multilayer is deposited using direct current magnetron sputtering technology. The reflectivity of the 1st-order Bragg peak measured at E = 8.05 keV is 38%, and the angular resolution (∆θ/θ) is less than 1.0%. Fitting results of the reflectivity curve indicate a layer thickness drift of 1.6%, mainly accounting for the broadening of the Bragg peaks. The layer morphology is further characterized by transmission electron microscopy, and a well-ordered multilayer structure with sharp interfaces is observed from the substrate to the surface. The material combination of WSi2/Si is a promising candidate for the fabrication of a high-resolution multilayer monochromator in the hard X-ray region.

show abstract

In Situ Loading and Time‐Resolved Synchrotron‐Based Phase Contrast Tomography for the Mechanical Investigation of Connective Knee Tissues: A Proof‐of‐Concept Study

Dejea,

Pierantoni,

Orozco

et al. 2024

Advanced Science

View full text Add to dashboard Cite

Articular cartilage and meniscus transfer and distribute mechanical loads in the knee joint. Degeneration of these connective tissues occurs during the progression of knee osteoarthritis, which affects their composition, microstructure, and mechanical properties. A deeper understanding of disease progression can be obtained by studying them simultaneously. Time‐resolved synchrotron‐based X‐ray phase‐contrast tomography (SR‐PhC‐µCT) allows to capture the tissue dynamics. This proof‐of‐concept study presents a rheometer setup for simultaneous in situ unconfined compression and SR‐PhC‐µCT of connective knee tissues. The microstructural response of bovine cartilage (n = 16) and meniscus (n = 4) samples under axial continuously increased strain, or two steps of 15% strain (stress–relaxation) is studied. The chondrocyte distribution in cartilage and the collagen fiber orientation in the meniscus are assessed. Variations in chondrocyte density reveal an increase in the top 40% of the sample during loading, compared to the lower half. Meniscus collagen fibers reorient perpendicular to the loading direction during compression and partially redisperse during relaxation. Radiation damage, image repeatability, and image quality assessments show little to no effects on the results. In conclusion, this approach is highly promising for future studies of human knee tissues to understand their microstructure, mechanical response, and progression in degenerative diseases.

show abstract

TOMCAT: A beamline for TOmographic Microscopy and Coherent rAdiology experimenTs

Cited by 100 publications

References 0 publications

High resolution tomographic imaging of the alveolar region of the mammalian lung

High resolution tomographic imaging of the alveolar region of the mammalian lung

Small d-spacing WSi2/Si multilayers for X-ray monochromators

In Situ Loading and Time‐Resolved Synchrotron‐Based Phase Contrast Tomography for the Mechanical Investigation of Connective Knee Tissues: A Proof‐of‐Concept Study

Contact Info

Product

Resources

About