Tomato (Solanum lycopersicum L.) SlIPT3 and SlIPT4 isopentenyltransferases mediate salt stress response in tomato

Žižková, Eva; Dobrev, Petre I.; Muhovski, Yordan; Hošek, Petr; Hoyerová, Klára; Haisel, D.; Procházková, Dagmar; Lutts, Stanley; Motyka, Václav; Hichri, Imène

doi:10.1186/s12870-015-0415-7

Cited by 72 publications

(39 citation statements)

References 56 publications

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“…Together this shows that cytokinin levels are increased in young tomato seedlings in response to salt stress. Similar increases in cytokinin levels have been previously found in connection to salt stress (Joshi et al., ; Prerostova et al., ; Šimura et al., ; Veselov et al., ; Žižková et al., ). However, there are also a number of studies where decreased levels of cytokinins are seen after salt stress.…”

Section: Discussionsupporting

confidence: 83%

“…Twenty‐two different cytokinin forms were detected in this study including precursors, active, transported, and conjugated forms of isopentenyladenine (iP), trans ‐zeatin ( tZ ), dihydrozeatin (DHZ), and cis ‐zeatin ( c Z). Cytokinin levels in buffer‐treated control plants show general similarities to those previously found in tomato (Žižková et al., ) and other eudicot plants with some variation (Sakakibara, ). The most abundant components are the N‐glucoside forms (iP7G, tZ7G, and DHZ7G), with the active bases iP, t Z, and cZ making up a small part of the total measured cytokinins (Figure ).…”

Section: Resultssupporting

confidence: 78%

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Salt and oxidative stresses uniquely regulate tomato cytokinin levels and transcriptomic response

et al. 2018

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Cytokinins are well‐known to be involved in processes responsible for plant growth and development. More recently, these hormones have begun to be associated with stress responses as well. However, it is unclear how changes in cytokinin biosynthesis, signaling, or transport relate to stress effects. This study examines in parallel how two different stresses, salt, and oxidative stress, affect changes in both cytokinin levels and whole plant transcriptome response. Solanum lycopersicum seedlings were given a short‐term (6 hr) exposure to either salt (150 mM NaCl) or oxidative (20 mM H2O2) stress and then examined to determine both changes in cytokinin levels and transcriptome. LC‐MS/MS was used to determine the levels of 22 different types of cytokinins in tomato plants including precursors, active, transported, and conjugated forms. When examining cytokinin levels we found that salt treatment caused an increase in both active and inactive cytokinin levels and oxidative stress caused a decrease in these levels. RNA‐sequencing analyses of these same stress‐treated tissues revealed 6,643 significantly differentially expressed genes (DEGs). Although many DEGs are similar between the two stresses, approximately one‐third of the DEGs in each treatment were unique to that stress. Several cytokinin‐related genes were among the DEGs. Examination of photosystem II efficiency revealed that cytokinins affect physiological response to stress in tomato, further validating the changes in cytokinin levels seen in planta.

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Section: Discussionsupporting

confidence: 83%

Section: Resultssupporting

confidence: 78%

Salt and oxidative stresses uniquely regulate tomato cytokinin levels and transcriptomic response

et al. 2018

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“…Determination and quantification of CKs and auxins were performed with a high-performance liquid chromatography (HPLC) system (Ultimate 3000, Dionex) coupled to a hybrid triple quadrupole/linear ion trap mass spectrometer (3200 Q TRAP, Applied Biosystems) using a multilevel calibration graph with [ 2 H]-labelled internal standards, as described previously (Dobrev et al , 2005; Djilianov et al , 2013; Žižková et al , 2015). Detection of 2-methylthio derivatives of iP, trans Z and their ribosides was set up in a selected reaction-monitoring mode.…”

Section: Methodsmentioning

confidence: 99%

Control of cytokinin and auxin homeostasis in cyanobacteria and algae

Žižková

Kubeš

Dobrev

et al. 2016

Ann Bot

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Background and Aims The metabolism of cytokinins (CKs) and auxins in vascular plants is relatively well understood, but data concerning their metabolic pathways in non-vascular plants are still rather rare. With the aim of filling this gap, 20 representatives of taxonomically major lineages of cyanobacteria and algae from Cyanophyceae, Xanthophyceae, Eustigmatophyceae, Porphyridiophyceae, Chlorophyceae, Ulvophyceae, Trebouxiophyceae, Zygnematophyceae and Klebsormidiophyceae were analysed for endogenous profiles of CKs and auxins and some of them were used for studies of the metabolic fate of exogenously applied radiolabelled CK, [3H]trans-zeatin (transZ) and auxin ([3H]indole-3-acetic acid (IAA)), and the dynamics of endogenous CK and auxin pools during algal growth and cell division.Methods Quantification of phytohormone levels was performed by high-performance or ultrahigh-performance liquid chromatography–electrospray tandem mass spectrometry (HPLC-MS/MS, UHPLC-MS/MS). The dynamics of exogenously applied [3H]transZ and [3H]IAA in cell cultures were monitored by HPLC with on-line radioactivity detection.Key Results The comprehensive screen of selected cyanobacteria and algae for endogenous CKs revealed a predominance of bioactive and phosphate CK forms while O- and N-glucosides evidently did not contribute greatly to the total CK pool. The abundance of cis-zeatin-type CKs and occurrence of CK 2-methylthio derivatives pointed to the tRNA pathway as a substantial source of CKs. The importance of the tRNA biosynthetic pathway was proved by the detection of tRNA-bound CKs during the course of Scenedesmus obliquus growth. Among auxins, free IAA and its oxidation catabolite 2-oxindole-3-acetic acid represented the prevailing endogenous forms. After treatment with [3H]IAA, IAA-aspartate and indole-3-acetyl-1-glucosyl ester were detected as major auxin metabolites. Moreover, different dynamics of endogenous CKs and auxin profiles during S. obliquus culture clearly demonstrated diverse roles of both phytohormones in algal growth and cell division.Conclusions Our data suggest the existence and functioning of a complex network of metabolic pathways and activity control of CKs and auxins in cyanobacteria and algae that apparently differ from those in vascular plants.

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“…Involvement of AVP1 in salt tolerance was previously demonstrated by its overexpression in both Arabidopsis and tomato (Gaxiola et al ., ; Yang et al ., ). Tomato SlIPT3 and SlIPT4 isopentenyltransferases (IPTs) take part in the salinity response by affecting cytokinin homeostasis, which among others affects leaf senescence (Žižková et al ., ). The tomato ARS1 ( altered response to salt stress 1 ) gene encodes a MYB transcription factor that contributes to reduced transpiration water loss under salt stress, which is mediated by ABA (Campos et al ., ).…”

Section: Tomato Tolerance To Salt Stressmentioning

confidence: 97%

Plant behaviour under combined stress: tomato responses to combined salinity and pathogen stress

et al. 2018

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SUMMARYCrop plants are subjected to a variety of stresses during their lifecycle, including abiotic stress factors such as salinity and biotic stress factors such as pathogens. Plants have developed a multitude of defense and adaptation responses to these stress factors. In the field, different stress factors mostly occur concurrently resulting in a new state of stress, the combined stress. There is evidence that plant resistance to pathogens can be attenuated or enhanced by abiotic stress factors. With stress tolerance research being mostly focused on plant responses to individual stresses, the understanding of a plant's ability to adapt to combined stresses is limited. In the last few years, we studied powdery mildew resistance under salt stress conditions in the model crop plant tomato with the aim to understand the requirements to achieve plant resilience to a wider array of combined abiotic and biotic stress combinations. We uncovered specific responses of tomato plants to combined salinitypathogen stress, which varied with salinity intensity and plant resistance genes. Moreover, hormones, with their complex regulation and cross-talk, were shown to play a key role in the adaptation of tomato plants to the combined stress. In this review, we attempt to understand the complexity of plant responses to abiotic and biotic stress combinations, with a focus on tomato responses (genetic control and cross-talk of signaling pathways) to combined salinity and pathogen stresses. Further, we provide recommendations on how to design novel strategies for breeding crops with a sustained performance under diverse environmental conditions.

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Tomato (Solanum lycopersicum L.) SlIPT3 and SlIPT4 isopentenyltransferases mediate salt stress response in tomato

Cited by 72 publications

References 56 publications

Salt and oxidative stresses uniquely regulate tomato cytokinin levels and transcriptomic response

Salt and oxidative stresses uniquely regulate tomato cytokinin levels and transcriptomic response

Control of cytokinin and auxin homeostasis in cyanobacteria and algae

Plant behaviour under combined stress: tomato responses to combined salinity and pathogen stress

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