Plant behaviour under combined stress: tomato responses to combined salinity and pathogen stress

Bai, Yuling; Kissoudis, Christos; Yan, Zhe; Visser, R.G.F.; Linden, Gerard van der

doi:10.1111/tpj.13800

Cited by 175 publications

(126 citation statements)

References 115 publications

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“…The activity of SOD, POD, APX and CAT was significantly increased in PI while it was reduced in R95 cultivar when treated with WS, HS, and WS+HS. The activities of GR were significantly higher in all three types of stresses in both Plants' responses to concurrent stresses often occurring in the field are very exclusive, when compared to individual stress treatments and will display cross-tolerance to better adapt to those stresses (54)(55). We report the result of our studies on two contrast soybean cultivars to determine the physiological and molecular mechanism for cross-tolerance between multiple stresses.…”

Section: Determination Of Enzymes Activitiesmentioning

confidence: 91%

Physiological, Proteomic, and Biochemical analysis Reveal Possible Cross-Tolerance in metabolism and heat response proteins in response to Heat and Water Stress in Soybean

Katam¹,

Murthy²,

Shokri³

et al. 2019

Preprint

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Water stress (WS) and heat stress (HS) have a negative effect on soybean plant growth and crop productivity. During WS, soybean plants opt for survival through ion homeostasis and the conformations of proteins are disconcerted as plant cells lose water while HS leads to difficulties in flowering and fruiting. Some of these changes include oxidative stress leading to the destruction of photosynthetic apparatus, macromolecules within cells and the onset of complex signaling cascades. Changes in the physiological characteristics, proteome, and certain metabolites investigated on molecular and cellular functions were studied in two soybean cultivars exposed to different heat and water stress conditions independently and in combination. Leaf protein composition was studied using 2-DE and complemented with MALDI TOF mass spectrometry. While two cultivars displayed genetic variation in response to water and heat stress, thirty-nine proteins were significantly altered in their relative abundance in response to WS, HS and combined WS+HS in both cultivars; a majority of them involved in metabolism, response to heat and photosynthesis showing significant cross-tolerance mechanisms. Functional analysis revealing a majority of heat responsive-proteins were more abundant during HS and combined stress (WS+HS) whereas these proteins were low to WS in cultivar PI 471938 and heat shock proteins were in low abundance to water, heat and combined stresses in cultivar R95-1705. Most protein abundances were not correlated with their expression at mRNA levels in PI cultivar, however, in cultivar R 95, the expression levels of transcript follow their relative abundance in proteins. Our systems bioinformatics analyses revealed that MED37C, a probable mediator of RNA polymerase transcription II protein showed potential interacting partners in Arabidopsis and our studies signifies the marked impact of this protein in PI cultivar. Elevated activities in antioxidant enzymes indicate that the PI-371938 cultivar has the ability to restore the oxidation levels and sustain the plant during the stress. Our study hypothesizes the plant’s development of cross-stress tolerance which will help foster the ongoing ventures in genetic modifications in stress tolerance.

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Section: Determination Of Enzymes Activitiesmentioning

confidence: 91%

Physiological, Proteomic, and Biochemical analysis Reveal Possible Cross-Tolerance in metabolism and heat response proteins in response to Heat and Water Stress in Soybean

Katam¹,

Murthy²,

Shokri³

et al. 2019

Preprint

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“…a single stress condition vs its control), there is a knowledge gap in the identification of QTLs involved in responses to combined stress. This aspect is of capital importance as it was shown that stress combination in tomato (biotic and/or abiotic) does not always have an additive effect regarding physiological, morphological, or biochemical responses compared with single stress (Rivero et al ., ; Bai et al ., ; Martinez et al ., ).…”

Section: Discovery Of Genetic Variations Underlying Major Traits In Tmentioning

confidence: 97%

Trait discovery and editing in tomato

2018

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Summary Tomato (Solanum lycopersicum), which is used for both processing and fresh markets, is a major crop species that is the top ranked vegetable produced over the world. Tomato is also a model species for research in genetics, fruit development and disease resistance. Genetic resources available in public repositories comprise the 12 wild related species and thousands of landraces, modern cultivars and mutants. In addition, high quality genome sequences are available for cultivated tomato and for several wild relatives, hundreds of accessions have been sequenced, and databases gathering sequence data together with genetic and phenotypic data are accessible to the tomato community. Major breeding goals are productivity, resistance to biotic and abiotic stresses, and fruit sensorial and nutritional quality. New traits, including resistance to various biotic and abiotic stresses and root architecture, are increasingly being studied. Several major mutations and quantitative trait loci (QTLs) underlying traits of interest in tomato have been uncovered to date and, thanks to new populations and advances in sequencing technologies, the pace of trait discovery has considerably accelerated. In recent years, clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 gene editing (GE) already proved its remarkable efficiency in tomato for engineering favorable alleles and for creating new genetic diversity by gene disruption, gene replacement, and precise base editing. Here, we provide insight into the major tomato traits and underlying causal genetic variations discovered so far and review the existing genetic resources and most recent strategies for trait discovery in tomato. Furthermore, we explore the opportunities offered by CRISPR/Cas9 and their exploitation for trait editing in tomato.

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“…Tomato (Solanum lycopersicum) is one of the most commonly cultivated vegetables in the world (http://faostat.fao.org), and it also serves as a model plant for studying plant-microbe interactions [21]. Ebb-and-flow systems have been used widely for the commercial cultivation of tomato plug seedlings because of its notable advantages.…”

Section: Introductionmentioning

confidence: 99%

Microbial community dynamics in the recirculating nutrient solution of tomato plug seedlings cultivated under ebb-and-flow system

Chen¹,

Li²,

Wang³

et al. 2019

Preprint

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Background: The ebb-and-flow system has ability to recirculate water and nutrients, and offers a good method to control nutrient leaching from greenhouses into the environment. However, the potential for the rapid spread of bacterial and fungal pathogens is the main hindrance for its adoption in vegetable seedlings production. Natural microflora has often shown a certain ability to suppress diseases.Results: Here, through 16S rRNA- and ITS1-targeted Illumina sequencing, the dynamic changes in bacterial and fungal communities in the recirculating nutrient solution were characterized for tomato plug seedlings cultivated in an ebb-and-flow system in summer and winter. Both bacterial number and microbial diversity in the nutrient solution increased with recirculating irrigation, and these changes differed between summer and winter. Pseudomonas was among the most predominant bacterial genera in the nutrient solution; its relative abundance gradually increased with recycling in summer but decreased dramatically in winter. In summer, the predominant bacteria also included Comamonas and Sediminibacterium , whose relative abundances in the nutrient solution decreased and increased, respectively. In winter, the nutrient solution was also colonized by Sphingomonas , predominantly at the late stage of irrigation. Among fungi, Amanita and Trichoderma were predominant in both summer and winter. Amanita gradually increased in summer but decreased rapidly after the first irrigation cycle in winter, whereas Trichoderma accumulated rapidly at the late stage of irrigation in both summer and winter. Alternaria and Fusarium were predominant in the nutrient solution only in summer, with the former decreased and the latter increased with recirculating irrigation. Moreover, Pseudomonas mediterranea , a potentially pathogenic bacterium, and Fusarium oxysporum , a fungal pathogen were present predominantly in winter and summer, respectively. Some potentially beneficial microbes functioning in plant-growth promotion and water self-purification, such as Sphingobium xenophagum , Trichoderma harzianum , and T. virens , were identified to be increased in the recirculating nutrient solution.Conclusions: The present data elucidate the bacterial and fungal dynamics in an ebb-and-flow system and provide useful information for pathogen control during tomato seedling production.

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Plant behaviour under combined stress: tomato responses to combined salinity and pathogen stress

Cited by 175 publications

References 115 publications

Physiological, Proteomic, and Biochemical analysis Reveal Possible Cross-Tolerance in metabolism and heat response proteins in response to Heat and Water Stress in Soybean

Physiological, Proteomic, and Biochemical analysis Reveal Possible Cross-Tolerance in metabolism and heat response proteins in response to Heat and Water Stress in Soybean

Trait discovery and editing in tomato

Microbial community dynamics in the recirculating nutrient solution of tomato plug seedlings cultivated under ebb-and-flow system

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