Tomato Endophytic Bacteria Composition and Mechanism of Suppressiveness of Wilt Disease (Fusarium oxysporum)

Abstract

Tomato wilt disease, caused by the Fusarium oxysporum is an ever-increasing threat for agricultural production, and unreasonable fertilization and pesticide abuse caused environmental challenge. Increasing evidence suggested that microbiomes or those associated with crops, played key roles on plant health. Plant disease dynamics were affected by multiple biotic and abiotic factors including phytopathogen population density, the genetic type of the pathogen and the host, in particular, the composition and assembly of the host-associated microbiome. However, it was unclear how pathogen invasion interaction and correlate with endophytic bacterial communities in natural field conditions. To study this, we sampled temporally the tomato plants that were exposed to F. oxysporum invasions over one crop season. High-throughput sequencing were performed to explore the correlation between agricultural practice, pathogen invasion, and endophytic microbiota communities. Results showed that pathogen invasion had clear effect on the endophytic and a strong link between increased pathogen densities and reduced abundance of Bacillus sp., which are crucial taxonomy for suppressiveness to F. oxysporum in vitro and in greenhouse condition. In summary, monitoring the dynamics of endophytic bacteria communities and densities of pathogen could thus open new avenue for more accurate disease diagnostics and high-efficiency screening antagonisms methods in the future, and our results will broaden the agricultural view of beneficial microbiota as biological control agents against plant pathogen.

Pseudomonas spp. Enriched in Endophytic Community of Healthy Cotton Plants Inhibit Cotton Verticillium Wilt

“…The enriched OTUs of healthy plants probably play an important role in protecting the plants from cotton Verticillium wilt. This finding is consistent with the previous report ( Yuan et al, 2020 ; Zhang et al, 2021 ). Furthermore, the results displayed that the healthy plants and resistant cultivars contained specific OTUs corresponding to Sphingobacteriaceae, which included the biocontrol genus of Sphingobacterium ( Xu et al, 2020 ).…”

Influence of Bacillus subtilis strain Z-14 on microbial communities of wheat rhizospheric soil infested with Gaeumannomyces graminis var. tritici

“…The number of links among members within a fungal community increased soon after the Ggt application, but decreased significantly later on, while strain Z-14 significantly increased the fungal community interactions (Table 3). Pathogen invasion significantly disrupted microbial associations when compared with the healthy tomato whose microbial associations were more complex (Zhang et al, 2021). In our study, more positive interactions were found with the inoculation of strain Z-14, indicating that synergies among microbes were enhanced.…”

Comparison of Rhizospheric and Endophytic Bacterial Compositions between Netted and Oriental Melons

“…Endophytic bacteria also play an important role in promoting plant growth. Moreover, endophytic bacteria can effectively enhance disease control ( 37 ). In this study, even though soil bacterial diversity and richness in the rhizosphere were not significantly different between netted and oriental melons, the endophytic bacterial diversity and richness in the stems of netted melons were significantly higher than those of oriental melons.…”