Replacement of primary old-growth forests by secondary woodlands in threatened subtropical biomes drives important changes at the level of the overstory, understory and forest floor, but the impact on belowground microbial biodiversity is yet poorly documented. In the present study, we surveyed by metabarcoding sequencing, the diversity and composition of soil bacteria and fungi in the old-growth forest, dominated by stone oaks (Lithocarpus spp.) and in the secondary Yunnan pine woodland of an iconic site for biodiversity research, the Ailaoshan National Nature Reserve (Ailao Mountains, Yunnan province, China). We assessed the effect of forest replacement and other environmental factors, including soil horizons, soil physicochemical characteristics and seasonality (monsoon vs. dry seasons). We showed that tree composition and variation in soil properties were major drivers for both bacterial and fungal communities, with a significant influence from seasonality. Ectomycorrhizal Operational Taxonomic Units (OTUs) dominated the functional fungal guilds. Species richness and diversity of the bacterial and fungal communities were higher in the pine woodland compared to the primary Lithocarpus forest, although prominent OTUs were different. The slightly lower complexity of the microbiome in the primary forest stands likely resulted from environmental filtering under relatively stable conditions over centuries, when compared to the secondary pine woodlands. In the old-growth forest, we found a higher number of species, but that communities were homogeneously distributed, whereas in the pine woodlands, there is a slightly lower number of species present but the communities are heterogeneously distributed. The present surveys of the bacterial and fungal diversity will serve as references in future studies aiming to assess the impact of the climate change on soil microbial diversity in both old-growth forests and secondary woodlands in Ailaoshan.
The plant microbiome plays a fundamental role in plant growth and health. However, detailed information regarding the plant endophytic microbiome during the infection period of a pathogen is largely unknown. Here, we investigated the microbial community of healthy and diseased cotton plants and the root exudate profiles of susceptible and resistant cultivars utilizing high-throughput sequencing and metabolomics. The results showed that the pathogen infection reduced bacterial diversity and significantly affected the bacterial community composition. The microbiome assembly is shaped predominantly by cultivars. The endophytic microbiome of the infected plants showed greater complexity than the healthy plants in network analysis. The results displayed that a total of 76 compounds were significantly different in the two groups, with 18 compounds showing a higher relative abundance in the resistant cultivars and 58 compounds in the susceptible cultivars. Pathway enrichment analysis showed that pathways related to plant hormone signal transduction, biosynthesis of various secondary metabolites, and biosynthesis and metabolism of amino acids were prominently altered. We also demonstrate that plants inoculated with Pseudomonas sp. strains showed increased resistance to the cotton Verticillium wilt compared with the control plants in pot experiments. Overall, it showed that the pathogen infection affected the community composition, and healthy plants displayed an enriched beneficial microbiome to combat the plant disease. These findings significantly advance our understanding of the endophytic microbiome assembly under the pathogen infection and develop microbiome-based solutions for sustainable crop production systems.
Spatial planning is a public policy arrangement for land use allocation and spatial structure regulation. As a method used by the public sector to influence the spatial distribution of future activities, spatial planning has become an important method and basis for the Chinese government to perform its duties. In the process of its long-term development, China has formed a unique spatial planning system. Based on the perspective of evolution and comparison, this paper systematically reviews the evolution of China’s spatial planning system from “multi-plan division” to “multi-plan integration” under the inheritance of departments. The findings are as follows. ① China’s spatial planning has long presented a pattern of separate management by multiple departments, such as development and reform, construction, land, and environmental protection. The emergence and development of various types of planning is a necessary spatial governance tool for specific national conditions and major issues of land space development and protection in China. ② In the evolution process of more than half a century, the planning of various departments has gradually established, inherited, and continuously changed their own planning systems and control content; thus, China’s spatial planning has undergone a process of “planning absence–planning division–planning integration”. ③ The brand-new territorial spatial plan inherits the “three types” of control space, including land utilization master planning, urban and rural master planning, and ecological environment planning, and forms a set of binding index systems, which have become the decision-making basis for the current territorial space resource allocation. ④ In the future, China’s spatial planning system should be further optimized and improved in aspects such as the coordination mechanism of “soft” and “hard” spatial planning, the spatial resource allocation system that places equal emphasis on legality and efficiency, and the spatial layout system from “major function-oriented zoning” to “space use zoning”. Insight into the evolution of China’s spatial planning system can provide historical and logical support for the improvement of China’s spatial governance thinking and the continuous improvement of the efficiency of land space resource allocation in the future and provide a certain reference value for the comparative study of the planning systems of different countries in the world.
A new poroid wood-inhabiting fungus in the family Irpicaceae, Irpex jinshaensis sp. nov., is described and illustrated from China based on morphological and molecular evidence. The species was collected in Yunnan Province, where it grew on the undersides of fallen angiosperm branches. I. jinshaensis is characterized by an annual growth habit, resupinate basidiocarp with white to cream pores when fresh becoming salmon to cinnamon upon drying, a monomitic hyphal system, generative hyphae thick-walled with simple septa, and occasionally covered with small crystal granules, the presence of encrusted cystidia and subglobose to globose basidiospores. The phylogenetic analysis based on ITS + nLSU rDNA sequences shows that the new species belongs to Irpex, clustering with I. hydnoides, I. hacksungii and I. lacteus with strong support. In addition, a new combination, I. subulatus, is proposed based on morphological and phylogenetic analyses. Morphological and molecular characters confirm the placement of both the new species and the combination in Irpex. A key to the species of Irpex known from China is provided.
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