Tomato and garlic by gavage modulate 7,12-dimethylbenz[a]anthracene-induced genotoxicity and oxidative stress in mice

Abstract

Chemoprotection by dietary agents is a promising strategy for cancer prevention. The aim of the present study was to evaluate the combined effect of tomato and garlic against 7,12-dimethylbenz-[a]anthracene (DMBA)-induced genetic damage and oxidative stress in 12-14-week-old male Swiss albino mice. The animals were randomized into experimental and control groups and divided into eight groups of five animals each. Group 1 animals were injected intraperitoneally with 35 mg/kg body weight DMBA suspended in peanut oil as a single dose. Groups 2-4 animals received tomato (500 mg/kg body weight), garlic (125 mg/kg body weight) and a combination of tomato and garlic for 5 days by gavage, respectively, followed by DMBA 1.5 h after the final feeding. The doses of tomato and garlic correspond to the average human daily consumption. Animals in groups 5, 6 and 7 received tomato alone, garlic alone and tomato + garlic combination, respectively, for 5 days. Group 8 animals received the same volume of water and served as control. The incidence of bone marrow micronuclei and the extent of lipid peroxidation and the concentrations of antioxidants glutathione, glutathione peroxidase and glutathione-S-transferase were measured in the liver, 48 h after DMBA exposure. Increased frequency of micronuclei and enhanced lipid peroxidation accompanied by compromised antioxidant defenses were observed in DMBA-treated animals. Although pretreatment with tomato or garlic significantly reduced the frequency of DMBAinduced bone marrow micronuclei, the combination of tomato and garlic exhibited more profound effect in inhibiting DMBA-induced genotoxicity and oxidative stress. We suggest that a broad spectrum of antimutagenic and anticlastogenic effects can be achieved through an effective combination of functional foods such as tomato and garlic.

Antigenotoxic Effect Of Ferulic Acid In 7,12-Dimethyl Benz(A)- Anthracene (Dmba) Induced Genotoxicity

“…A to T transversions in Ha-ras codon 61 has been demonstrated in DMBA induced mouse skin and hamster buccal pouch carcinogenesis (Chang et al, 1996). An increase in micronucleus frequency and chromosomal aberrations in the bone marrow of DMBA painted or injected rodents have been reported earlier (Bhuvaneswari et al, 2004). Osaka et al, (1996) have suggested that N-ras mutation is an earliest event in DMBA induced leukemogenesis.…”

“…It has been demonstrated that DMBA exposure results in pronounced mutagenic response in several in vivo and in vitro mutation assay system. Polyploidy and sister chromatic exchanges have been shown in DMBA induced genotoxicity (Han et al, 2007;Bhuvaneswari et al, 2004;Chandramohan et al, 2003).…”

Therapeutic Effect of Hydro-Ethanolic Extract of Pothos scandens L on key Carbohydrate Metabolizing Enzymes and Xenobiotic Marker Enzymes in DMBA Induced Experimental Mammary Carcinoma

“…33 It has been shown that DMBA-induced mammary carcinoma leads to disruption of redox balance of tissues which cause undesirable consequences to the mitochondrial membrane suggesting that biochemical and physiological alterations may result from oxidative damage. 34,35 Non malignant cells depend primarily on mitochondrial oxidative phosphorylation (OXPHOS) than aerobic glycolysis for its energy requirement. However, Otto Warburg suggested that in contrast to non malignant cells, malignant cells depend on glycolysis rather than OXPHOS for ATP generation which is considered by some as the seventh hallmark of cancer.…”

Plantas medicinais no tratamento do câncer: uma breve revisão de literatura

“…O alho também possui atividade antioxidante que, atualmente, vem sendo bastante estudada (BHUVANESWARI et al, 2004).…”