Tomatidine suppresses inflammation in primary articular chondrocytes and attenuates cartilage degradation in osteoarthritic rats

Abstract

In this study, we investigated whether the anti-inflammatory effects of tomatidine alleviate osteoarthritis (OA)-related pathology in primary articular chondrocytes and a rat OA model. STITCH database analysis identified 22 tomatidine-target genes that were enriched in 78 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Moreover,39 of the 105 OA-related KEGG pathways were related to tomatidine-target genes. The top two OA-related KEGG pathways with tomatidine-target genes were the MAPK and neutrophin signaling pathways. Pretreating primary chondrocytes with tomatidine suppressed interleukin-1β (IL-1β)-induced expression of iNOS, COX-2, MMP1, MMP3, MMP13, and ADAMTS-5. Tomatidine also suppressed IL-1β-induced degradation of collagen-II and aggrecan proteins by inhibiting NF-κB and MAPK signaling. In a rat OA model, histological and immunohistochemical analyses showed significantly less cartilage degeneration in thetibiofemoral joints of rats treated for 12 weeks with tomatidine after OA induction (experimental group) than in untreated OA group rats. However, micro-computed tomography (μ-CT) showed that tomatidine did not affect remodeling of the subchondral bone at the tibial plateau. These data shows that tomatidine suppresses IL-1β-induced inflammation in primary chondrocytes by inhibiting the NF-κB and MAPK signaling pathways, and protects against cartilage destruction in a rat OA model.

ALPK1 Accelerates the Pathogenesis of Osteoarthritis by Activating NLRP3 Signaling

“…In contrast, the systemic knockout of ALPK1 probably breaks the dynamic equilibrium of osteoblasts and osteoclasts in the subchondral bone, although alleviation of cartilage degradation arises. Similarly, Chu and colleagues ( 42 ) discovered that the systemic administration of tomatidine via drinking attenuated cartilage degradation in rats with OA, without improving the destruction of the subchondral bone in the tibia.…”

Tomatidine Improves Pulmonary Inflammation in Mice with Acute Lung Injury

“…Tomatidine is thought to attenuate the inflammatory response in LPS-induced macrophages and articular chondrocytes [12,20]. Previous studies have demonstrated that tomatidine can induce apoptosis effects in osteosarcoma, lung cancer, and breast cancer [11,[21][22][23].…”

Anti-Parasitic Activity of Cherry Tomato Peel Powders

“…Kozukue et al [ 12 ] reported on the analysis of the tetrasaccharide glycoalkaloids α-tomatine and dehydrotomatine as well the corresponding aglycones tomatidine and tomatidenol lacking the carbohydrate side chain, as illustrated in Figure 2 of vegetative parts (calyxes, flowers, green and red tomato fruit, leaves, roots and stems) of greenhouse-grown cherry (mini) and two standard tomatoes using HPLC and mass spectrometry. The analytical method for tomatidine is of interest because it can be used in metabolism and pharmacology studies of reported beneficial biological properties, including anti-trichomonad [ 4 ], antiviral [ 13 ], anti-inflammatory [ 14 ], and anti-cancer effects [ 15 ]. Friedman et al [ 16 ] reported on the analysis of peel powders from six commercial cherry tomato varieties for free amino acid, phenolic, flavonoid, β-carotene, lycopene, and glycoalkaloid content using HPLC/MS (mass spectrometry).…”