Toll-like receptors, IFN-γ and IL-12 expression in bovine leukemia virus-infected animals with low or high proviral load

Farías, María Victoria Nieto; Lendez, Pamela Anahí; Marin, Maia Solange; Quintana, Silvina; Martinez-Cuesta, Lucía; Ceriani, María Carolina; Dolcini, Guillermina Laura

doi:10.1016/j.rvsc.2016.06.016

Cited by 29 publications

(24 citation statements)

References 59 publications

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“…In contrast, specifically in the S+E group, a stimulation of the production of IL-12, i.e. of the cytokine which is crucial for the induction of the Th1 response, was recorded (Farias et al, 2016). In this study a stimulation of IL-6 and TNF-α production was observed in all of the vaccinated groups, but the response varied with the different adjuvant formulations.…”

contrasting

confidence: 59%

Analysis of the immune response of calves to various saponin-based adjuvants for an experimental Mycoplasma bovis vaccine

Dudek

Bednarek

Ayling

et al. 2018

Acta Veterinaria Hungarica

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Mycoplasma bovis is a primary infectious agent of many disorders in cattle including bovine respiratory disease. No commercial vaccines against M. bovis are available in Europe. The immune response of calves to three saponin-based adjuvants combined with a field Polish M. bovis strain was evaluated. Four groups of six calves each were injected subcutaneously with the M. bovis strain combined with either saponin, saponin + Emulsigen®, saponin + Emulsigen® + alphatocopherol acetate, or with phosphate-buffered saline as control group. Blood and nasal swab samples were collected up to day 84 post injection. All formulations effectively stimulated the humoral and the cellular immune response of the calves, but the course of the response depended on the adjuvant formulation. These immunological data provide additional information supporting the findings of previous M. bovis saponin and Emulsigen® vaccine challenge studies to facilitate the development of successful M. bovis vaccines.

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contrasting

confidence: 59%

Analysis of the immune response of calves to various saponin-based adjuvants for an experimental Mycoplasma bovis vaccine

Dudek

Bednarek

Ayling

et al. 2018

Acta Veterinaria Hungarica

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“…Blood samples were obtained from an adult Holstein (Holando-Argentino) dairy cow belonging to a herd from Tandil region (Provincia de Buenos Aires, Argentina). Previous studies (Farias et al, 2016) demonstrated that this animal was BLV infected and carried a high proviral load (HPL). For peripheral blood mononuclear cells (PBMCs) separation, 10 ml of heparinized blood (5U/ml) were obtained by jugular venipuncture.…”

Section: Sample Collectionmentioning

confidence: 84%

“…An absolute quantification method by real-time PCR (qPCR) was used for proviral load determination as described previously (Farias et al, 2016). A plasmid carrying the complete BLV genome was used for the construction of the standard curve.…”

Section: Proviral Load Determinationmentioning

confidence: 99%

Stable infection of a bovine mammary epithelial cell line (MAC-T) with bovine leukemia virus (BLV)

et al. 2018

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Bovine leukemia virus (BLV) is a retrovirus that affects cattle causing a lymphoproliferative disease. BLV infection has been associated with misbalance of the immune response causing a higher incidence of other infections. Mastitis is one of the most important conditions that affect milk production in cattle. The aim of this study was to stably infect a bovine mammary epithelial cell line (MAC-T). MAC-T cell line was successfully infected with BLV and the infection was confirmed by nested PCR, qPCR, immunocytochemistry, western blot and transmission electron microscopy. This is the first report of a bovine mammary epithelial cell line stably infected with BLV. This new cell line could be used as an in vitro model to study the effect of BLV on the immune response in the mammary gland and the relationship with other agents causing mastitis.

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“…The absolute quantification method by qPCR used for proviral load (PVL) determination was the one described by Farias et al (2016). The standard curve was constructed using a plasmid carrying one copy of the entire BLV genome under control of its own promoter LTR (pBLV), with six 10-fold serial dilutions of pBLV, containing 1 million to 10 BLV copies.…”

Section: Proviral Load Determination By Real-time Pcr (Qpcr)mentioning

confidence: 99%

“…The standard curve was constructed using a plasmid carrying one copy of the entire BLV genome under control of its own promoter LTR (pBLV), with six 10-fold serial dilutions of pBLV, containing 1 million to 10 BLV copies. The qPCR conditions for pol amplification were previously described (FARIAS et al, 2016). Each reaction contained 30 ng of DNA from BLVinfected animals.…”

Section: Proviral Load Determination By Real-time Pcr (Qpcr)mentioning

confidence: 99%

A novel association of BoLA DRB3 alleles in BLV infected cattle with different proviral loads

Farías

Caffaro

Lendez

et al. 2017

Braz. J. Vet. Res. Anim. Sci.

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Bovine leukemia virus (BLV) is associated with the most common neoplastic disease of cattle. BLV has a silent dissemination in the herd due to infected cell exchange, thus the concentration of BLV-infected cells in blood should play a major role in the success of viral transmission. Genes from Bovine leukocyte antigen (BoLA), the MHC system of cattle, are associated with genetic resistance and susceptibility to a wide range of diseases, and also with production traits. Some BoLA DRB3.2 allele polymorphisms in Holstein cattle have been associated with resistance or susceptibility to BLV-disease development, or with proviral load (PVL). This investigation studied 107 BLV-infected Argentinean Holstein dairy cows, all of them belonging to one herd. PVL was analysed by qPCR and animals were classified as high proviral load (HPVL, N = 88) and low proviral load (LPVL, N = 19), and BoLA DRB3.2 alleles were genotyped. Alleles BoLA DRB3.2*1501 and *1201 were significantly associated with HPVL (p = 0.0230 and p = 0.0111 respectively), while allele BoLA DRB3.2*0201 was significantly associated with LPVL (p = 0.0030). The present study aims at contributing to the knowledge of the association between BoLA polymorphism and development of a BLV infection profile. Genes that best explain the PVL in this population resulted BoLA DRB3.2*0201 (as a protection factor) and *1501 (as a risk factor). Allelic differences may play an important role in the development of effective immune responses. A better understanding of how BoLA polymorphism contributes to these responses and the establishment of a BLV status is desirable to schedule and evaluate control measures. Keywords: BLV. Proviral load. BoLA DRB3 polymorphism. ResumoO vírus da leucemia bovina (BLV) está associado à doença neoplásica mais comum do gado bovino. O BLV tem uma disseminação silenciosa no rebanho devido à troca de células infectadas, assim, a concentração de células BLV infectadas no sangue deve desempenhar um papel importante no sucesso da transmissão viral. Os genes do antígeno leucocitário bovino (BoLA), sistema MHC do gado bovino, estão associados à resistência genética e à susceptibilidade a uma ampla gama de doenças, bem como às características da produção. Alguns polimorfismos de alelos de BoLA DRB3.2 em bovinos Holstein têm sido associados à resistência ou susceptibilidade ao desenvolvimento da doença BLV, ou com carga proviral (PVL). Esta investigação avaliou 107 vacas leiteiras da raça Holstein argentina infectadas com BLV e pertencentes a um único rebanho. A PVL foi analisada por qPCR, os animais foram classificados em alta carga proviral (HPVL, N = 88) e baixa carga proviral (LPVL, N = 19) | 216Braz.

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Toll-like receptors, IFN-γ and IL-12 expression in bovine leukemia virus-infected animals with low or high proviral load

Cited by 29 publications

References 59 publications

Analysis of the immune response of calves to various saponin-based adjuvants for an experimental Mycoplasma bovis vaccine

Analysis of the immune response of calves to various saponin-based adjuvants for an experimental Mycoplasma bovis vaccine

Stable infection of a bovine mammary epithelial cell line (MAC-T) with bovine leukemia virus (BLV)

A novel association of BoLA DRB3 alleles in BLV infected cattle with different proviral loads

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