Tolerance to heavy metal stress in seedlings of three pine species from contrasting environmental conditions in Chile

Arencibia, Ariel D.; Rodríguez, Carmen Dolores Arbelo; Roco, Lisandro; Vergara, Carolina; González-Soto, N.; García‐González, Ricardo

doi:10.3832/ifor1908-009

Cited by 3 publications

(3 citation statements)

References 41 publications

(42 reference statements)

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“…Plant traits' transgenerational inheritance for adaptation to environmental changes is known. In a study on different pine trees species, the hypothesis that abiotic stress in the maternal environment can induce an inherited "transgenerational plasticity" was proven [21]. The potential of this capacity for practical applications is easy to imagine [22].…”

Section: Discussionmentioning

confidence: 99%

Dittrichia viscosa Selection Strategy Based on Stress Produces Stable Clonal Lines for Phytoremediation Applications

Anglana,

Capaci,

Barozzi

et al. 2023

Plants

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Dittrichia viscosa uptake and translocation of the metalloid As is not fully understood and some data are contradictory, but its adaptability to this pollutant is known and is dependent on its genetic variability. D. viscosa is not a hyperaccumulator plant, but it can grow in high-drought conditions while still producing large biomass, even tolerating significant concentrations of As3+ and As5+. In spite of these remarkable characteristics, adaptive modification of performances is not predictable in wild populations. In previous work, we established experimental clonal populations to perform a functional study on the aquaporin NIP1.1. Here, we propose a strategy to select a clonal population of D. viscosa with a defined phenotype related to As tolerance and to reduced NIP1.1 expression levels for phytoremediation applications. From the previous work, we selected four independent clones, two of them belonging to the weak population (W8 and W9) and the other two belonging to the strong population (S1 and S3). The weak and strong populations differ for a different expression ratio root/shoot of DvNip1;1 that brings a different tolerance to As presence. The stress response of the populations, revealed by the CAT enzymatic test, was statistically correlated to the clones, but not to As uptake. Performance of the selected plants on a second unrelated metallic pollutant, Cd, was evaluated, showing that Cd uptake is also independent from the tolerant phenotype. In vitro culture methods using solid media and temporary immersion bioreactors were compared to propose an optimized combined protocol. The procedure yielded propagation of genetically stable tolerant clonal lines with good uptake of As and Cd. The plants, mass-produced with the developed in vitro protocol, were able to maintain their acquired abilities and are potentially able be later applied in phytoremediation or contaminated areas’ re-naturalization.

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Section: Discussionmentioning

confidence: 99%

Dittrichia viscosa Selection Strategy Based on Stress Produces Stable Clonal Lines for Phytoremediation Applications

Anglana,

Capaci,

Barozzi

et al. 2023

Plants

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“…Among numerous plant species, isolated populations have adapted to settle in metalliferous soils and at constant presence of elevated levels of metals (Muszyńska et al 2013;Arencibia et al 2016;Bothe and Słomka 2017). Some other species are capable of hyperaccumulating HM ions taken up from the soil.…”

Section: Introductionmentioning

confidence: 99%

“…Reactions associated with tolerance and sensitivity traits can be distinguished via comparative studies on species/ genotypes/lines exhibiting different level of tolerance to stress factors (Arencibia et al 2016;Ferrer et al 2018;Wei et al 2018;Koźmińska et al 2019). Although attempts have been made to elucidate HM tolerance mechanisms using this approach, there is limited information on the efficiency of plant acclimation to HM stress using prolonged, constant exposure to these ions.…”

Section: Introductionmentioning

confidence: 99%

In vitro acclimation to prolonged metallic stress is associated with modulation of antioxidant responses in a woody shrub Daphne jasminea

Wiszniewska

Muszyńska

Kołton

et al. 2019

Plant Cell Tiss Organ Cult

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The study aimed at distinguishing antioxidant responses conditioning acclimation status to metallic stress in a shoot culture of a woody plant Daphne jasminea. Growth, organogenesis, metal accumulation, oxidative stress level and antioxidant responses were compared in the long-term treated shoots (Lt lines) and short-term treated (St lines) with Cd, Ni and Pb in relation to control shoots. Growth rate and organogenic potential were higher in the long-term treated Lt Cd and Lt Pb lines than in respective short-term treated St lines. Lt Ni line proliferated less effectively than St Ni line, whereas biomass accretion was similar in both Ni lines. All heavy metal (HM)-exposed shoots suffered from oxidative stress, however, it was more severe in St than in Lt lines. In the long-term treated lines three major factors were associated with the acclimation status: activity of peroxidase (POD) and accumulation of anthocyanins and carotenoids. POD activity was 4-1.5-times higher in Lt lines than in the control one. Anthocyanin content nearly tripled, while carotenoid content doubled in Lt lines. Other elements of antioxidant system, CAT and SOD, glutathione, ascorbic acid and proline, complemented the action of the "major players" in long-term treated Lt lines depending on heavy metal type. Short-term treated lines responded by accumulation of proteins and a decline in catalase activity. The study demonstrated that long-term heavy metal treatment can be considered acclimation strategy, during which substantial modulation of the antioxidant system occurs. Due to orchestrated action of cytosolic POD, vacuolar anthocyanins and membrane-bound carotenoids HM toxicity could be counteracted in various cellular compartments. Key message Acclimation to heavy metal exposure in D. jasminea shoot cultures involves modulation of antioxidant activity and is conditioned by an orchestrated action of cellular peroxidases, vacuolar anthocyanins and membrane-bound carotenoids.

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Plants facing oxidative challenges—A little help from the antioxidant networks

Soares

Carvalho

Fidalgo

2019

Environmental and Experimental Botany

312

215

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Tolerance to heavy metal stress in seedlings of three pine species from contrasting environmental conditions in Chile

Cited by 3 publications

References 41 publications

Dittrichia viscosa Selection Strategy Based on Stress Produces Stable Clonal Lines for Phytoremediation Applications

Dittrichia viscosa Selection Strategy Based on Stress Produces Stable Clonal Lines for Phytoremediation Applications

In vitro acclimation to prolonged metallic stress is associated with modulation of antioxidant responses in a woody shrub Daphne jasminea

Plants facing oxidative challenges—A little help from the antioxidant networks

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