Tolerance as Shown by the Simonsen Reaction on the Chorioallantoic Membrane

Abstract

SUMMARY By prenatal administration of embryonic spleen cells, chickens can be rendered tolerant in the sense that their circulating leucocytes are incapable of producing focal lesions on the chorioallantoic membranes of embryos of genotypes corresponding to those of the cell donors. In most of the experiments reported the extent of tolerance diminished rapidly as the birds grew older but in some of the pure line chickens persisting tolerance suggests that on occasion the effect may last indefinitely. The significance of antigenic specificity in the Simonsen reaction is briefly discussed.

The Frequency of Antigen-Sensitive Cells in Tissue Transplantation

“…Pretreatment of the donor in such a way that is is rendered specifically tolerant of the host precludes the otherwise regular development of a GVH reaction. This important principle was first demonstrated for GVH reactions in chicken embryos in Burnet's laboratory (15). It seems to be as clear a proof as can be devised that the GVH reactions observed were not a nonimmunological cell contact phenomenon.…”

Genetic analysis of transplantation immunity in the fowl

“…Hilgard et al (1962) recently studied tolerance phenom enon with respect to the CAM reaction. They found that by prenatal administration of embryonic spleen cells, chickens could be made tolerant so that their leukocytes were later in capable of producing lesions on the CAM of embryos whose geno types corresponded to those of the spleen cell donors.…”