Tolerability and immunogenicity of an eleven valent mixed carrier Streptococcus pneumoniae capsular polysaccharide-diphtheria toxoid or tetanus protein conjugate vaccine in Finnish and Israeli infants

Dagan, Ron; Käyhty, Helena; Wuorimaa, Tomi; Yaich, Mansour; Bailleux, Fabrice; Zamir, Orly; Eskola, Juhani

doi:10.1097/01.inf.0000109221.50972.53

Cited by 48 publications

(24 citation statements)

References 28 publications

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“…In addition to PCV7, pneumococcal conjugate vaccines have recently been formulated that contain 9 (7, 19), 11 (8), or 13 serotypes. These new-generation vaccines contain antigen to serotype 5 and are in various stages of clinical trials.…”

Section: Discussionmentioning

confidence: 99%

Genotypic Analysis of Invasive Streptococcus pneumoniae from Mali, Africa, by Semiautomated Repetitive-Element PCR and Pulsed-Field Gel Electrophoresis

et al. 2007

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Of the 12 serotypes present, the majority of isolates were not contained in PCV7 (the 7-valent pneumococcal conjugate vaccine), including 1 isolate that was serotype 1, 12 isolates that were serotype 2, 58 isolates that were serotype 5, 7 isolates that were serotype 7F, and 1 isolate that was serotype 12F. To determine whether clonal dissemination of the predominant serotypes had taken place, genotyping was performed on 100 S. pneumoniae isolates by using two methods: pulsed-field gel electrophoresis (PFGE) of SmaI-digested genomic DNA, and the Bacterial Barcodes repetitive-element PCR (rep-PCR) method. Criteria for delineating rep-PCR genotypes were established such that isolates of different serotypes were generally not grouped together. The two methods were equally discriminatory within a given pneumococcal serotype. PFGE separated the isolates into 15 genotypes and 7 subtypes; rep-PCR separated isolates into 15 genotypes and 6 subtypes. Using either method, isolates within serotypes 2, 5, and 7 formed three large, separate clusters containing 1 genotype each. Both methods further distinguished related subtypes within serotypes 2 and 5. Interestingly, one of the PFGE subtypes of serotype 5 is indistinguishable from the Columbia 5 -19 clone circulating in Latin America since 1994. The data support that serotypes 2 and 5 were likely to be the result of dissemination of particular clones, some of which are responsible for invasive disease over a broad population range.

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Section: Discussionmentioning

confidence: 99%

Genotypic Analysis of Invasive Streptococcus pneumoniae from Mali, Africa, by Semiautomated Repetitive-Element PCR and Pulsed-Field Gel Electrophoresis

et al. 2007

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“…La vigilancia de la prevalencia y dispersión de este clon en el país y en otros países de Latinoamérica y del mundo (3-6) resulta útil para evaluar la presión selectiva que pueda generarse por la introducción de las vacunas conjugadas que incluyan el serotipo 5 (7,8). Por tal razón, el objetivo de este estudio fue establecer, por medio de la determinación de los patrones de restricción de PFGE, las relaciones genéticas entre los aislamientos invasores de S. pneumoniae serotipo 5 recuperados entre 1994 y 2004 y el clon 19-Colombia 5 , con el fin de actualizar la información y contribuir con el programa de vigilancia molecular del neumococo.…”

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Caracterización molecular de aislamientos invasores colombianos de Streptococcus pneumoniae serotipo 5 recuperados entre 1994 y 2004.

2006

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Introducción. Streptococcus pneumoniae serotipo 5 es causa importante de enfermedad invasora en Colombia, donde se ha demostrado la circulación del clon 19-Colombia 5 susceptible a penicilina pero resistente a tetraciclina y a cloranfenicol. Objetivo. Establecer las relaciones genéticas de aislamientos invasores colombianos de S. pneumoniae serotipo 5 recuperados entre 1994 y 2004 con el clon 19-Colombia 5 . Materiales y métodos. Se estudiaron 83 aislamientos que tenían datos de susceptibilidad a penicilina, vancomicina, ceftriaxona, eritromicina, trimetoprim sulfametoxazol, cloranfenicol y tetraciclina, de los cuales 29 fueron obtenidos de niños menores de cinco años. El patrón de restricción del ADN se determinó por electroforesis en gel de campo pulsado, usando la enzima Sma I. La similitud genética entre los aislamientos y el clon se estableció según los criterios de Tenover y utilizando el programa Fingerprinting II. Resultados. Todos los aislamientos se relacionaron con el clon 19-Colombia 5 y se agruparon en el patrón electroforético A con 17 subtipos. El patrón A se estableció en 32 aislamientos (38,6%), el A8 en 18 (21,7%) y el A5 en 10 (12%), los 15 patrones restantes agruparon los otros 23 aislamientos. Los 34 aislamientos resistentes a tetraciclina y cloranfenicol mostraron relación con los patrones electroforéticos A (n = 32), A16 (n = 1) y A28 (n = 1), caracterizados, al igual que el clon, por presentar una banda de 340 Kb. Conclusión. Estos resultados muestran la circulación continua en el país de aislamientos de S. pneumoniae serotipo 5 genéticamente relacionados con el clon 19-Colombia 5 .Palabras clave: Streptococcus pneumoniae, meningitis, neumonía, electroforesis en gel de campo pulsado, resistencia a la tetraciclina, resistencia a cloranfenicol. Molecular characterization of Colombian invasive serotype 5 Streptococcus pneumoniae isolates recovered between 1994 and 2004Background. Streptococcus pneumoniae serotype 5 is an important cause of invasive disease in Colombia where the circulation of the clone Colombia 5 -19, penicillin susceptible but tetracycline and chloramphenicol resistant, has been demonstrated. Objective. To establish the genetic relatedness among Colombian invasive S. pneumoniae serotype 5 isolates, recovered between 1994 and 2004, and the clone Colombia 5 -19. Materials and methods. Eighty three isolates with penicillin, vancomycin, ceftriaxone, erythromycin, trimethoprim-sulfametoxazole, chloramphenicol and tetracycline susceptibility patterns were studied. Of these isolates, 29 were recovered from children less than 5 years of age. DNA restriction patterns of all isolates were determined for pulsed field gel electrophoresis, using the SmaI enzyme. Genetic similarity among isolates and the clone Colombia 5 -19 was established according to Tenover's criteria and the Fingerprintingä II program. Results. All isolates were related with the Colombia 5 -19 clone and belonged to the electrophoretic pattern A, of which 17 subtypes were derived. Most of the isolates belonged to pat...

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“…Current vaccines comprise epidemiologically dominant serotype-based selections of pneumococcal capsular polysaccharides, conjugated or not to a carrier protein (Dagan et al, 2004;Fedson & Musher, 2004;Mbelle et al, 1999;Smart et al, 1987). However, the vaccine formulations do not cover all serotypes of S. pneumoniae, which might be of particular relevance in certain regions that have different dominant serotypes (Dagan et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional regulation, occurrence and putative role of the Pht family of Streptococcus pneumoniae

Rioux¹,

Neyt²,

Paolo³

et al. 2011

Microbiology

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Restricted to the genus Streptococcus, the Pht protein family comprises four members: PhtA, PhtB, PhtD and PhtE. This family has the potential to provide a protein candidate for incorporation in pneumococcal vaccines. Based on sequence analysis and on RT-PCR experiments, we show here that the pht genes are organized in tandem but that their expression, except that of phtD, is monocistronic. PhtD, PhtE, PhtB and PhtA are present in 100, 97, 81 and 62 % of the strains, respectively, and, by analysing its sequence conservation across 107 pneumococcal strains, we showed that PhtD displays very little variability. To analyse the physiological function of these proteins, several mutants were constructed. The quadruple Pht-deficient mutant was not able to grow in a poor culture medium, but the addition of Zn 2+ or Mn 2+ restored its growth capacity.Moreover, the phtD mRNA expression level increased when the culture medium was depleted in zinc. Therefore, we suggest that these proteins are zinc and manganese scavengers, and are able to store these metals and to release them when the bacterium faces an ion-restricted environment. The data also showed that this protein family, and more particularly PhtD, is a promising candidate to be incorporated into pneumococcal vaccines.

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Tolerability and immunogenicity of an eleven valent mixed carrier Streptococcus pneumoniae capsular polysaccharide-diphtheria toxoid or tetanus protein conjugate vaccine in Finnish and Israeli infants

Abstract: PncDT11 is safe and immunogenic in infants. The use of 11-valent pneumococcal vaccine would increase the serotype coverage beyond the currently available 7-valent vaccine.

Cited by 48 publications

References 28 publications

Genotypic Analysis of Invasive Streptococcus pneumoniae from Mali, Africa, by Semiautomated Repetitive-Element PCR and Pulsed-Field Gel Electrophoresis

Genotypic Analysis of Invasive Streptococcus pneumoniae from Mali, Africa, by Semiautomated Repetitive-Element PCR and Pulsed-Field Gel Electrophoresis

Caracterización molecular de aislamientos invasores colombianos de Streptococcus pneumoniae serotipo 5 recuperados entre 1994 y 2004.

Transcriptional regulation, occurrence and putative role of the Pht family of Streptococcus pneumoniae

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