Togaviruses in Cultured Arthropod Cells

Stollar, Victor

doi:10.1016/b978-0-12-625380-1.50025-4

Cited by 38 publications

(38 citation statements)

References 75 publications

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“…In vitro sialylation restored the hemagglutination titre of this virus to levels approaching those of the same virus grown in BHK-21 cells. Impaired sialylation has also been observed for the Semliki forest virus E2 glycoprotein produced by mosquito cells (Stollar, 1980) and purified Sind-bis virus grown in mosquito cells, relative to the same virus grown in BHK cells (Stollar et al, 1976;Hirsch et al, 1981).…”

Section: Glycoprotein Sialylation During Viral Infectionmentioning

confidence: 91%

Glycoproteins from Insect Cells: Sialylated or Not?

Marchal

Jarvis²,

Cacan³

et al. 2001

Biological Chemistry

159

122

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Our growing comprehension of the biological roles of glycan moieties has created a clear need for expression systems that can produce mammalian-type glycoproteins. In turn, this has intensified interest in understanding the protein glycosylation pathways of the heterologous hosts that are commonly used for recombinant glycoprotein expression. Among these, insect cells are the most widely used and, particularly in their role as hosts for baculovirus expression vectors, provide a powerful tool for biotechnology. Various studies of the glycosylation patterns of endogenous and recombinant glycoproteins produced by insect cells have revealed a large variety of O-and Nlinked glycan structures and have established that the major processed O-and N-glycan species found on these glycoproteins are (Galβ1,3)GalNAc-O-Ser/Thr and Man3(Fuc)GlcNAc2-N-Asn, respectively. However, the ability or inability of insect cells to synthesize and compartmentalize sialic acids and to produce sialylated glycans remains controversial. This is an important issue because terminal sialic acid residues play diverse biological roles in many glyco-conjugates. While most work indicates that insect cell-derived glycoproteins are not sialylated, some wellcontrolled studies suggest that sialylation can occur. In evaluating this work, it is important to recognize that oligosaccharide structural determination is tedious work, due to the infinite diversity of this class of compounds. Furthermore, there is no universal method of glycan analysis; rather, various strategies and techniques can be used, which provide gly-cobiologists with relatively more or less precise and reliable results. Therefore, it is important to consider the methodology used to assess glycan structures when evaluating these studies. The purpose of this review is to survey the studies that have contributed to our current view of glycoprotein sialylation in insect cell systems, according to the methods used. Possible reasons for the disagreement on this topic in the literature, which include the diverse origins of biological material and experimental artifacts, will be discussed. In the final analysis, it appears that if insect cells have the genetic potential to perform sialylation of glycoproteins, this is a highly specialized function that probably occurs rarely. Thus, the production of sialylated recombinant glycoproteins in the baculovirus-insect cell system will require metabolic engineering efforts to extend the native protein glycosylation pathways of insect cells.

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Section: Glycoprotein Sialylation During Viral Infectionmentioning

confidence: 91%

Glycoproteins from Insect Cells: Sialylated or Not?

Marchal

Jarvis²,

Cacan³

et al. 2001

Biological Chemistry

159

122

View full text Add to dashboard Cite

show abstract

“…Huang (1982) speculated that the main- tenance of neurovirulent strains in nature may require cycling through biological hosts, such as young birds (ducklings), which sustain brain infections, and that attenuated strains arise by persistent inherited (transovarial) infection of mosquitoes. The latter hypothesis is given weight by laboratory (Stollar and Shenk, 1973;Stollar, 1980;Kuno, 1982) and field studies (Reeves et al, 1958) with mosquito-borne togaviruses, which indicate that viruses from persistently infected arthropods or arthropod cells develop markers of attenuation (reduced neurovirulence, small plaque size, temperature lability or sensitivity). Oligonucleotide mapping of JE strains from Thailand (Burke et al, 1985a) indicated a high level of genotypic conservation among isolates from brains of fatal human cases compared to isolates from mosquito vectors.…”

Section: Pathobiological Significance Of Natural Virus Variationmentioning

confidence: 99%

Pathobiology of the Flaviviruses

Monath

1986

The Togaviridae and Flaviviridae

105

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“…Flavivirus infection of arthropod cells can be either cytocidal or noncytocidal (Mussgay et al, 1975;Trent and Naeve, 1980). Some flaviviruses induce syncytium formation in mosquito-cell lines (Igarashi, 1978(Igarashi, , 1979Ng and Westaway, 1980;Stollar, 1980). Flavivirus progeny produced by some types of arthropod cells have been found to differ phenotypically from the parental virus used to infect the culture.…”

Section: Infection Of Cultured Cellsmentioning

confidence: 99%

Replication of Flaviviruses

Brinton

1986

The Togaviridae and Flaviviridae

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Togaviruses in Cultured Arthropod Cells

Cited by 38 publications

References 75 publications

Glycoproteins from Insect Cells: Sialylated or Not?

Glycoproteins from Insect Cells: Sialylated or Not?

Pathobiology of the Flaviviruses

Replication of Flaviviruses

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