Replication of Flaviviruses

Brinton, Margo A.

doi:10.1007/978-1-4757-0785-4_11

Cited by 84 publications

(53 citation statements)

References 162 publications

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“…Flaviviruscs, which include yellow fcvcr, Japanese encephalitis (JE) and dengue (DEN) viruscs, can rcplicatc in a widc variety of culturcd cells of vcrtcbratc and arthropod origins (Brinton, 1986). Flavivirus-spccificd protein and RNA synthcsis, in addition to virion asscmbly, occur in the pcrinuclcar region of the infected cells (Wcstaway & Ng, 1980;Ng et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

“…Flavivirus-spccificd protein and RNA synthcsis, in addition to virion asscmbly, occur in the pcrinuclcar region of the infected cells (Wcstaway & Ng, 1980;Ng et al, 1983). It is generally acknowledged that thcrc is no specific nuclcar involvcmcnt in the flavivirus replication (Brinton, 1986). Howcvcr, conflicting results have bccn obtained, showing that enuclcatcd cells failed to.…”

Section: Introductionmentioning

confidence: 99%

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Detection of Dengue 4 Virus Core Protein in the Nucleus. I. A Monoclonal Antibody to Dengue 4 Virus Reacts with the Antigen in the Nucleus and Cytoplasm

Tadano

Makino

Fukunaga

et al. 1989

Journal of General Virology

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SUMMARYA mouse monoclonal antibody (MAb) to dengue 4 (DEN-4) virus reacted with the antigen in the nucleus as well as in the cytoplasm of DEN-4-infected mammalian and mosquito cells, as demonstrated by the peroxidase-antiperoxidase staining method. The intranuclear antigen appeared to accumulate at the nucleoli, forming spots, whereas the cytoplasmic antigen appeared to be localized mainly in large perinuclear loci in the infected cells. The MAb-reactive antigen was produced in the presence of actinomycin D, which caused the accumulation in the nucleus to be altered to a dispersed pattern. Radioimmunoprecipitation analysis of [35S]methionine-labelled purified virions and Western blot analysis of the antigens prepared from the infected mammalian and mosquito cells showed that the MAb was directed against the DEN-4 virus core protein (M~ 15-5K). These results indicated that the DEN-4 virus core protein was partially transported, soon after its synthesis in the cytoplasm, into the nucleus and accumulated at the nucleoli.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection of Dengue 4 Virus Core Protein in the Nucleus. I. A Monoclonal Antibody to Dengue 4 Virus Reacts with the Antigen in the Nucleus and Cytoplasm

Tadano

Makino

Fukunaga

et al. 1989

Journal of General Virology

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“…By analogy, the maturation of flaviviruses also occurs on intracellular membranes and there is no evidence that the plasma membrane plays any role in replication or maturation (Brinton, 1986). In spite of this, it was found that both the non-structural NS1 and the envelope E proteins are present on the surface of cells infected with the flavivirus yellow fever virus (Schlesinger et al, 1990).…”

mentioning

confidence: 99%

Immunofluorescence Studies of Biotype-Specific Expression of Bovine Viral Diarrhoea Virus Epitopes in Infected Cells

Greiser‐Wilke¹,

Dittmar

Ließ³

et al. 1991

Journal of General Virology

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“…Production of flavivirus particles in infected cells occurs rapidly, less than 12 h PI; and whole particles are associated with perinuclear cisternae of endoplasmic reticulum vesicles (9,85). Viral nucleocapsids have not been detected within infected cells (9,14). Therefore, rapid nucleocapsid assembly and membrane association are supported.…”

Section: P R O C E S S I N G O F T H E F L a V I V I R U S P O L Y P mentioning

confidence: 99%

“…8). Concurrent with tlavivirus translocation and release from cells, a proteolytic cleavage event takes place in which the aminoterminal portion of the preM protein is released forming the mature M protein (9,14). This proteolytic event is required to produce fully infectious flavivirus particles (113).…”

Section: P R O C E S S I N G O F T H E F L a V I V I R U S P O L Y P mentioning

confidence: 99%

Insect-transmitted vertebrate viruses: Flaviviridae

Ludwig

Iacono-Connors

1993

In Vitro Cell Dev Biol - Animal

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SUMMARYThe Flaviviridae include almost 70 viruses, nearly half of which have been associated with human disease. These viruses are among the most important arthropod-borne viruses worldwide and include dengue, yellow fever, and Japanese encephalitis viruses. Morbidity and mortality caused by these viruses vary, but collectively they account for millions of encephalitis, hemorrhagic fever, arthralgia, rash, and fever cases per year. Most of the members of this family are transmitted between vertebrate hosts by arthropod vectors, most commonly mosquitoes or ticks. Transmission cycles can be simple or complex depending on the hosts, vectors, the virus, and the environmental factors affecting both hosts and viruses. Replication of virus in invertebrate hosts does not seem to result in any significant pathology, which suggests a close evolutionary relationship between virus and vector. Another example of this relationship is the ability of these viruses to grow in invertebrate ceil culture, where replication usually results in a steady state, persistent infection, often without cytopathic effect. Yields of virus from insect cell culture vary but are generally similar to yields in vertebrate cells. Replication kinetics are comparable between insect and vertebrate ceil lines, despite differences in incubation temperature. Both vertebrate and insect cell culture systems continue to play a significant role in flavivirus isolation and the diagnosis of disease caused by these agents. Additionally, these culture systems permit the study of flavivirus attachment, penetration, replication, and release from cells and have been instrumental in the production and characterization of live-attenuated vaccines. Both vertebrate and insect cell culture systems will continue to play a significant role in basic and applied flavivirus research in the future.

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Replication of Flaviviruses

Cited by 84 publications

References 162 publications

Detection of Dengue 4 Virus Core Protein in the Nucleus. I. A Monoclonal Antibody to Dengue 4 Virus Reacts with the Antigen in the Nucleus and Cytoplasm

Detection of Dengue 4 Virus Core Protein in the Nucleus. I. A Monoclonal Antibody to Dengue 4 Virus Reacts with the Antigen in the Nucleus and Cytoplasm

Immunofluorescence Studies of Biotype-Specific Expression of Bovine Viral Diarrhoea Virus Epitopes in Infected Cells

Insect-transmitted vertebrate viruses: Flaviviridae

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