Tocopherol biosynthesis is enhanced in photomixotrophic sunflower cell cultures

Fachechi, Christian; Nisi, Rossella; Gala, Rosa; Leone, Antonella; Caretto, Sofia

doi:10.1007/s00299-006-0268-5

Cited by 14 publications

(9 citation statements)

References 26 publications

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“…It has been shown that tocopherols protect the dormant and germinating seeds in A. thaliana against oxidative degradation of lipids ( Sakuragi et al., 2006 ). The elevated levels of α-tocopherol in the chloroplast membranes can also be associated to the ability of tocopherols to quench ROS, hence protecting the photosynthetic apparatus from oxygen toxicity ( Fachechi et al., 2007 ). It is also reported to be involved in the regulation of photosynthesis and macronutrient uptake and utilization ( Sakuragi et al., 2006 ; Fritsche et al., 2017 ; Shaikh et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Photosynthetic Carbon Partitioning and Metabolic Regulation in Response to Very-Low and High CO2 in Microchloropsis gaditana NIES 2587

et al. 2020

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Photosynthetic organisms fix inorganic carbon through carbon capture machinery (CCM) that regulates the assimilation and accumulation of carbon around ribulose-1,5bisphosphate carboxylase/oxygenase (Rubisco). However, few constraints that govern the central carbon metabolism are regulated by the carbon capture and partitioning machinery. In order to divert the cellular metabolism toward lipids and/or biorenewables it is important to investigate and understand the molecular mechanisms of the CO 2-driven carbon partitioning. In this context, strategies for enhancement of CO 2 fixation which will increase the overall biomass and lipid yields, can provide clues on understanding the carbon assimilation pathway, and may lead to new targets for genetic engineering in microalgae. In the present study, we have focused on the physiological and metabolomic response occurring within marine oleaginous microalgae Microchloropsis gaditana NIES 2587, under the influence of very-low CO 2 (VLC; 300 ppm, or 0.03%) and high CO 2 (HC; 30,000 ppm, or 3% v/v). Our results demonstrate that HC supplementation in M. gaditana channelizes the carbon flux toward the production of long chain polyunsaturated fatty acids (LC-PUFAs) and also increases the overall biomass productivities (up to 2.0 fold). Also, the qualitative metabolomics has identified nearly 31 essential metabolites, among which there is a significant fold change observed in accumulation of sugars and alcohols such as galactose and phytol in VLC as compared to HC. In conclusion, our focus is to understand the entire carbon partitioning and metabolic regulation within these photosynthetic cell factories, which will be further evaluated through multiomics approach for enhanced productivities of biomass, biofuels, and bioproducts (B3).

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Section: Discussionmentioning

confidence: 99%

Photosynthetic Carbon Partitioning and Metabolic Regulation in Response to Very-Low and High CO2 in Microchloropsis gaditana NIES 2587

et al. 2020

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“…This cell line also showed a significantly higher tocopherol level than the starting sunflower cells (Fig. 2), revealing the possibility to enhance tocopherol production by favouring the photosynthetic potential of sunflower cells [31].…”

Section: Tocopherol Biosynthesis and Photomixotrophic Culture Conditionsmentioning

confidence: 97%

Tocopherol production in plant cell cultures

Caretto

Nisi

Paradiso

et al. 2010

Molecular Nutrition Food Res

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Tocopherols, collectively known as vitamin E, are lipophilic antioxidants, essential dietary components for mammals and exclusively synthesized by photosynthetic organisms. Of the four forms (alpha, beta, gamma and delta), alpha-tocopherol is the major vitamin E form present in green plant tissues, and has the highest vitamin E activity. Synthetic alpha-tocopherol, being a racemic mixture of eight different stereoisomers, always results less effective than the natural form (R,R,R) alpha-tocopherol. This raises interest in obtaining this molecule from natural sources, such as plant cell cultures. Plant cell and tissue cultures are able to produce and accumulate valuable metabolites that can be used as food additives, nutraceuticals and pharmaceuticals. Sunflower cell cultures, growing under heterotrophic conditions, were exploited to establish a suitable in vitro production system of natural alpha-tocopherol. Optimization of culture conditions, precursor feeding and elicitor application were used to improve the tocopherol yields of these cultures. Furthermore, these cell cultures were useful to investigate the relationship between alpha-tocopherol biosynthesis and photomixotrophic culture conditions, revealing the possibility to enhance tocopherol production by favouring sunflower cell photosynthetic properties. The modulation of alpha-tocopherol levels in plant cell cultures can provide useful hints for a regulatory impact on tocopherol metabolism.

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“…Studies conducted on the production of tocopherols by cell suspension cultures are seldom and generally focused on seed oil plants [ 2 , 17 ]. Although fresh grape [ 18 ], grape seed, pomace [ 19 ] and grape seed oil [ 20 – 22 ] had tocopherols in different amounts, with our best knowledge, there is no research on tocopherol accumulation in the grape cell suspension cultures.…”

Section: Introductionmentioning

confidence: 99%

The effects of cadmium chloride on secondary metabolite production in Vitis vinifera cv. cell suspension cultures

et al. 2014

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BackgroundPlant secondary metabolites are possess several biological activities such as anti-mutagenic, anti-carcinogenic, anti-aging, etc. Cell suspension culture is one of the most effective systems to produce secondary metabolites. It is possible to increase the phenolic compounds and tocopherols by using cell suspensions. Studies on tocopherols production by cell suspension cultures are seldom and generally focused on seed oil plants. Although fresh grape, grape seed, pomace and grape seed oil had tocopherols, with our best knowledge, there is no research on tocopherol accumulation in the grape cell suspension cultures. In this study, it was aimed to determine the effects of cadmium chloride treatments on secondary metabolite production in cell suspension cultures of grapevine. Cell suspensions initiated from callus belonging to petiole tissue was used as a plant material. Cadmium chloride was applied to cell suspension cultures in different concentration (1.0 mM and 1.5 mM) to enhance secondary metabolite (total phenolics, total flavanols, total flavonols, trans-resveratrol, and α-, β-, γ- δ-tocopherols) production. Cells were harvested at two days intervals until the 6th day of cultures. Amounts of total phenolics, total flavanols and total flavonols; trans-resveratrol and tocopherols (α-, β-, γ- and δ-tocopherols) and dry cell weights were determined in the harvested cells.ResultsPhenolic contents were significantly affected by the sampling time and cadmium concentrations. The highest values of total phenolic (168.82 mg/100 g), total flavanol (15.94 mg/100 g), total flavonol (14.73 mg/100 g) and trans-resveratrol (490.76 μg/100 g) were found in cells treated with 1.0 mM CdCl2 and harvested at day 2. Contents of tocopherols in the cells cultured in the presence of 1.0 mM CdCl2 gradually increased during the culture period and the highest values of α, β and γ tocopherols (145.61, 25.52 and 18.56 μg/100 g) were detected in the cell cultures collected at day 6.ConclusionsAs a conclusion, secondary metabolite contents were increased by cadmium chloride application and sampling time, while dry cell weights was reduced by cadmium chloride treatments.

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Tocopherol biosynthesis is enhanced in photomixotrophic sunflower cell cultures

Cited by 14 publications

References 26 publications

Photosynthetic Carbon Partitioning and Metabolic Regulation in Response to Very-Low and High CO2 in Microchloropsis gaditana NIES 2587

Photosynthetic Carbon Partitioning and Metabolic Regulation in Response to Very-Low and High CO2 in Microchloropsis gaditana NIES 2587

Tocopherol production in plant cell cultures

The effects of cadmium chloride on secondary metabolite production in Vitis vinifera cv. cell suspension cultures

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