Tobacco Transcription Factor TGA2.2 Is the Main Component of as-1-binding Factor ASF-1 and Is Involved in Salicylic Acid- and Auxin-inducible Expression of as-1-containing Target Promoters

Niggeweg, Ricarda; Thurow, Corinna; Kegler, Carsten; Gatz, Christiane

doi:10.1074/jbc.m909267199

Cited by 129 publications

(166 citation statements)

References 48 publications

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“…It has been shown that salicylic acid and auxins can induce plant genes through the interaction of inducible transcription factors with the as-1-related ciselements (Chen and Singh, 1999;Niggeweg et al, 2000). The Tnt1C sequence analyzed here contains a nearly palindromic sequence within the U3 region that closely resembles the as-1 element (TGACGTCAnnnnTGACGTCA), and this could be important for the induction of this Tnt1 subfamily by auxins and SA.…”

Section: The U3 Regions Of the Three Tnt1 Subfamilies Contain Promotementioning

confidence: 98%

“…It has been already shown that salicylic acid and 2,4-D can induce plant gene expression through as-1-related elements (Chen and Singh, 1999;Niggeweg et al, 2000). It is interesting that although sequence variability among the Tnt1C sequences amplified is high and two different subfamilies can be defined, all of them maintain a consensus as-1-related element within the U3 region.…”

Section: Retrotransposons As Models To Analyze Stressassociated Exprementioning

confidence: 99%

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Three Tnt1 Subfamilies Show Different Stress-Associated Patterns of Expression in Tobacco. Consequences for Retrotransposon Control and Evolution in Plants

et al. 2001

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The genomes of most Nicotiana species contain three different subfamilies of the Tnt1 retrotransposon, which differ completely in their U3 sequence, whereas the rest of the sequence is relatively constant. The results presented here show that all three Tnt1 subfamilies are expressed in tobacco (Nicotiana tabacum) and that the U3 sequence variability correlates with differences in the pattern of expression of the Tnt1 elements. Each of the three Tnt1 subfamilies is induced by stress, but their promoters have a different response to different stress-associated signaling molecules. The Tnt1A subfamily is particularly strongly induced by elicitors and methyl jasmonate, whereas expression of the Tnt1C subfamily is more sensitive to salicylic acid and auxins. The direct relationship between U3 sequence variability and differences in the stress-associated expression of the Tnt1 elements present in a single host species gives support to our model that postulates that retrotransposons have adapted to their host genomes through the evolution of highly regulated promoters that mimic those of the stress-induced plant genes. Moreover, here we show that the analysis of the transcriptional control of a retrotransposon population such as Tnt1 provides new insights into the study of the complex and still poorly understood network of defense-and stress-induced plant signal transduction pathways.

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Section: The U3 Regions Of the Three Tnt1 Subfamilies Contain Promotementioning

confidence: 98%

Section: Retrotransposons As Models To Analyze Stressassociated Exprementioning

confidence: 99%

Three Tnt1 Subfamilies Show Different Stress-Associated Patterns of Expression in Tobacco. Consequences for Retrotransposon Control and Evolution in Plants

et al. 2001

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“…Transgenic plants overexpressing these TGA mutants showed different phenotypes. In one study, overexpression of a tobacco TGA2.2 mutant (a homolog of Arabidopsis TGA2 ) diminished SA-and auxininducible PR gene induction (Niggeweg et al, 2000a). In another study, in which an Arabidopsis TGA2 mutant was overexpressed, PR gene induction was enhanced further (Pontier et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

In Vivo Interaction between NPR1 and Transcription Factor TGA2 Leads to Salicylic Acid–Mediated Gene Activation in Arabidopsis

2002

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The Arabidopsis NPR1 protein is a key regulator of salicylic acid (SA)-mediated gene expression in systemic acquired resistance. Based on yeast two-hybrid analysis, NPR1 has been suggested to interact with members of the TGA family of transcription factors, including TGA2 (AHBP-1b). However, genetic evidence demonstrating that the NPR1-TGA interaction occurs in planta is still lacking, and the role of this interaction in SA-mediated gene activation has yet to be determined. In this study, we expressed a truncated form of TGA2 in Arabidopsis and found that the resulting transgenic lines displayed phenotypes similar to those of npr1 mutants. This dominant-negative effect of the TGA2 mutant shows that TGA2 and NPR1 interact in planta. We also present biochemical evidence indicating that this interaction is specific and enhanced by SA treatment. Moreover, using a chimera reporter system, we found that a chimeric TGA2GAL4 transcription factor activated a UAS GAL :: GUS reporter gene in response to SA and that this activation was abolished in the npr1 mutant. NPR1 is required for the DNA binding activity of the transcription factor. These genetic data clearly demonstrate that TGA2 is a SA-responsive and NPR1-dependent transcription activator.

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“…Higher affinity binding to the Z-BACϩ of a class I TGA-type bZIP protein, PvTGA1.1, compared with two class II proteins, PvTGA2.1 and PvTGA2.2 (Fig. 4), is in contrast to that for the as-1 element that favors binding of a class II protein (Niggeweg et al, 2000a). High-affinity binding of class I compared with class II proteins may be an important difference between the Z-BAC and as-1 elements that is also manifested in a difference in the AZ-specific and auxin-regulated expression of the Z-BAC element (Fig.…”

Section: Discussionmentioning

confidence: 55%

“…A major component of the ASF-1-binding factor that binds to the as-1 element ) is a TGA-type class II bZIP protein (Niggeweg et al, 2000a). Higher affinity binding to the Z-BACϩ of a class I TGA-type bZIP protein, PvTGA1.1, compared with two class II proteins, PvTGA2.1 and PvTGA2.2 (Fig.…”

Section: Discussionmentioning

confidence: 99%

Functional Analysis of Regulatory Elements in the Gene Promoter for an Abscission-Specific Cellulase from Bean and Isolation, Expression, and Binding Affinity of Three TGA-Type Basic Leucine Zipper Transcription Factors

et al. 2002

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Site-directed mutagenesis was used to identify cis-acting elements that control hormonal and abscission-specific expression of the bean (Phaseolus vulgaris) abscission cellulase (BAC) promoter. Auxin inhibition of BAC promoter expression is at least in part controlled by a negatively regulated element and ethylene induction by a positively regulated element. One of a series of 15 different 10-bp mutations created in a 2.9-kb BAC promoter reduced reporter gene expression by 60%. The native sequence for this 10-bp mutation includes a TGA-type basic leucine zipper (bZIP) motif. Tandem ligation of three 18-bp BAC elements (Z-BAC), which includes the bZIP motif to a minimal Ϫ50 35S cauliflower mosaic virus promoter, enhanced expression in abscission zones (AZs) 13-fold over that of the minimal promoter alone. The native forward orientation of the Z-BAC elements was essential for high expression levels. Expression of the Z-BAC minimal construct was 3-fold greater in AZ than stems when compared with the expression levels of an internal control with an enhanced 35S cauliflower mosaic virus promoter. Polymerase chain reaction was used to identify three TGA-type bZIP transcription factors in an AZ cDNA library. One of these factors was of the class I type and two of the class II type. RNA-blot analysis was completed for these genes and electrophoretic mobility shift assays used to confirm their binding to the Z-BAC element. Electrophoretic mobility shift assay-binding affinity was greatest for the class I TGA-type bZIP factor. The results indicate a complex interaction of negative and positive regulating transcription factors that control BAC gene expression.

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Tobacco Transcription Factor TGA2.2 Is the Main Component of as-1-binding Factor ASF-1 and Is Involved in Salicylic Acid- and Auxin-inducible Expression of as-1-containing Target Promoters

Cited by 129 publications

References 48 publications

Three Tnt1 Subfamilies Show Different Stress-Associated Patterns of Expression in Tobacco. Consequences for Retrotransposon Control and Evolution in Plants

Three Tnt1 Subfamilies Show Different Stress-Associated Patterns of Expression in Tobacco. Consequences for Retrotransposon Control and Evolution in Plants

In Vivo Interaction between NPR1 and Transcription Factor TGA2 Leads to Salicylic Acid–Mediated Gene Activation in Arabidopsis

Functional Analysis of Regulatory Elements in the Gene Promoter for an Abscission-Specific Cellulase from Bean and Isolation, Expression, and Binding Affinity of Three TGA-Type Basic Leucine Zipper Transcription Factors

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