Tobacco susceptibility to Potato virus YNTN infection is affected by grafting and endogenous cytokinin content

Spoustová, Petra; Hýsková, Veronika; Müller, Karel; Schnablová, Renáta; Ryšlavá, Helena; Čeřovská, Noemi; Malbeck, Jiří; Cvikrová, Milena; Synková, Helena

doi:10.1016/j.plantsci.2015.02.017

Cited by 22 publications

(23 citation statements)

References 82 publications

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“…The SA pathway confers resistance to the Potyviruses PVY in potato 72 and peanut mottle virus (PeMV) 73 . In tobacco, plants primed with cytokinins became tolerant to the strain PVY NTN of potato virus Y (PVY) 66 . In this study involving cassava and UCBSV, both histidine kinases and regulatory proteins were among the over-expressed genes in Namikonga at 2 dag and 5 dag (Table 4 ).…”

Section: Discussionmentioning

confidence: 99%

A time series transcriptome analysis of cassava (Manihot esculenta Crantz) varieties challenged with Ugandan cassava brown streak virus

Amuge

Berger

Katari

et al. 2017

Sci Rep

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A time-course transcriptome analysis of two cassava varieties that are either resistant or susceptible to cassava brown streak disease (CBSD) was conducted using RNASeq, after graft inoculation with Ugandan cassava brown streak virus (UCBSV). From approximately 1.92 billion short reads, the largest number of differentially expressed genes (DEGs) was obtained in the resistant (Namikonga) variety at 2 days after grafting (dag) (3887 DEGs) and 5 dag (4911 DEGs). At the same time points, several defense response genes (encoding LRR-containing, NBARC-containing, pathogenesis-related, late embryogenesis abundant, selected transcription factors, chaperones, and heat shock proteins) were highly expressed in Namikonga. Also, defense-related GO terms of ‘translational elongation’, ‘translation factor activity’, ‘ribosomal subunit’ and ‘phosphorelay signal transduction’, were overrepresented in Namikonga at these time points. More reads corresponding to UCBSV sequences were recovered from the susceptible variety (Albert) (733 and 1660 read counts per million (cpm)) at 45 dag and 54 dag compared to Namikonga (10 and 117 cpm respectively). These findings suggest that Namikonga’s resistance involves restriction of multiplication of UCBSV within the host. These findings can be used with other sources of evidence to identify candidate genes and biomarkers that would contribute substantially to knowledge-based resistance breeding.

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Section: Discussionmentioning

confidence: 99%

A time series transcriptome analysis of cassava (Manihot esculenta Crantz) varieties challenged with Ugandan cassava brown streak virus

Amuge

Berger

Katari

et al. 2017

Sci Rep

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“…The role of CK in the induction of plant defense had been recognized only in the late 2000s [17,93]. Currently, data have been accumulated on the role of CK in the development of plant resistance to biotrophic [94], hemibiotrophic [17,95], and necrotrophic [96,97] pathogens of bacterial or fungal origin, as well as viruses [98]. Nevertheless, the regulatory mechanism and targets of cytokinins during defense responses to pathogens are still elusive.…”

Section: Sntox3 and Ethylene Inhibited Biosynthesis Modulated Metabolism And Activated Oxidative Degradation Of Cytokininsmentioning

confidence: 99%

Ethylene-Cytokinin Interaction Determines Early Defense Response of Wheat against Stagonospora nodorum Berk.

et al. 2021

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Ethylene, salicylic acid (SA), and jasmonic acid are the key phytohormones involved in plant immunity, and other plant hormones have been demonstrated to interact with them. The classic phytohormone cytokinins are important participants of plant defense signaling. Crosstalk between ethylene and cytokinins has not been sufficiently studied as an aspect of plant immunity and is addressed in the present research. We compared expression of the genes responsible for hormonal metabolism and signaling in wheat cultivars differing in resistance to Stagonospora nodorum in response to their infection with fungal isolates, whose virulence depends on the presence of the necrotrophic effector SnTox3. Furthermore, we studied the action of the exogenous cytokinins, ethephon (2-chloroethylphosphonic acid, ethylene-releasing agent) and 1-methylcyclopropene (1-MCP, inhibitor of ethylene action) on infected plants. Wheat susceptibility was shown to develop due to suppression of reactive oxygen species production and decreased content of active cytokinins brought about by SnTox3-mediated activation of the ethylene signaling pathway. SnTox3 decreased cytokinin content most quickly by its activated glucosylation in an ethylene-dependent manner and, furthermore, by oxidative degradation and inhibition of biosynthesis in ethylene-dependent and ethylene-independent manners. Exogenous zeatin application enhanced wheat resistance against S. nodorum through inhibition of the ethylene signaling pathway and upregulation of SA-dependent genes. Thus, ethylene inhibited triggering of SA-dependent resistance mechanism, at least in part, by suppression of the cytokinin signaling pathway.

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“…Peroxidase (POD, EC 1.11.1.7) isozyme pattern was assessed after separation by 10% native PAGE. POD isozymes were detected in situ by staining gels in 1 M acetate buffer, pH 4.6, with 0.04% benzidine and 10 mM H 2 O 2 [ 37 ].…”

Section: Methodsmentioning

confidence: 99%

Novel Insights into the Effect of Pythium Strains on Rapeseed Metabolism

et al. 2020

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Pythium oligandrum is a unique biological control agent. This soil oomycete not only acts as a mycoparasite, but also interacts with plant roots and stimulates plant defense response via specific elicitors. In addition, P. oligandrum can synthetize auxin precursors and stimulate plant growth. We analyzed the secretomes and biochemical properties of eleven Pythium isolates to find a novel and effective strain with advantageous features for plants. Our results showed that even closely related P. oligandrum isolates significantly differ in the content of compounds secreted into the medium, and that all strains secrete proteins, amino acids, tryptamine, phenolics, and hydrolytic enzymes capable of degrading cell walls (endo-β-1,3-glucanase, chitinase, and cellulase), exoglycosidases (especially β-glucosidase), proteases, and phosphatases. The most different strain was identified as a not yet described Pythium species. The changes in metabolism of Brassica napus plants grown from seeds coated with the tested Pythium spp. were characterized. Enhanced levels of jasmonates, ethylene precursor, and salicylic acid may indicate better resistance to a wide variety of pathogens. Glucosinolates, as defense compounds against insects and herbivores, were enhanced in young plants. Altogether, P. oligandrum strains varied in their life strategies, and either they could perform equally as plant growth promoters and mycoparasites or they had developed one of these strategies better.

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Tobacco susceptibility to Potato virus YNTN infection is affected by grafting and endogenous cytokinin content

Cited by 22 publications

References 82 publications

A time series transcriptome analysis of cassava (Manihot esculenta Crantz) varieties challenged with Ugandan cassava brown streak virus

A time series transcriptome analysis of cassava (Manihot esculenta Crantz) varieties challenged with Ugandan cassava brown streak virus

Ethylene-Cytokinin Interaction Determines Early Defense Response of Wheat against Stagonospora nodorum Berk.

Novel Insights into the Effect of Pythium Strains on Rapeseed Metabolism

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