Tobacco mosaic virus multiplication and expression of the N gene in necrotic responding tobacco varieties

Otsuki, Yoshiaki; Shimomura, Toshiyasu; Takebe, Itaru

doi:10.1016/0042-6822(72)90344-3

Cited by 137 publications

(44 citation statements)

References 16 publications

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“…It has been proposed that MP accumulation in PD is required for Tm-2 2 resistance and Tm- 2 2 blocks cell-to-cell movement of viruses (Meshi et al, 1989). In addition, the N gene-mediated resistance against TMV is also not expressed in protoplasts (Otsuki et al, 1972), but both N and its Avr protein p50 localize in the cytoplasm and nucleus, and p50 does not participate in cell-to-cell movement. In this study, we found that two MP mutants N5 (DN3-5 amino acids) and C81 (1-187 amino acids) were still able to induce HR cell death in transgenic Tm-2 2 N. benthamiana plants (Fig.…”

Section: Tm-2 2 Functions Independent Of Pd Localization Of Viral Mpmentioning

confidence: 99%

Antiviral Resistance Protein Tm-2² Functions on the Plasma Membrane

et al. 2017

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Section: Tm-2 2 Functions Independent Of Pd Localization Of Viral Mpmentioning

confidence: 99%

Antiviral Resistance Protein Tm-2² Functions on the Plasma Membrane

et al. 2017

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“…It was also demonstrated that a pulse of cAMP was induced between 20 and 40 h after TMV inoculation (Rosenberg et al, 1982), which is correlated with the time of AVF induction (Antignus et al, 1977) and of the beginning of local lesion production (Otsuki et al, 1972).…”

Section: Antiviral Activity Induced In Leaves and Callus Cultures Ofmentioning

confidence: 99%

Polyinosinic{middle dot}Polycytidylic Acid in Association with Cyclic Nucleotides Activates the Antiviral Factor (AVF) in Plant Tissues

Gat-Edelbaum

Altman

Sela

1983

Journal of General Virology

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SUMMARYLeaves and callus cultures from Nicotiana glutinosa L. were treated with a mixture of polyinosinic.policytidylic acid [poly(I)-poly(C)], cyclic AMP and cyclic GMP. A new antiviral activity appearing in the treated tissues co-purified with the antiviral factor AVF. Poly(I). poly(C) can replace tobacco mosaic virus infection in the induction of AVF activity, the latter being also mediated to a certain extent by cyclic nucleotides. The kinetics of the stimulation of AVF in these in vivo studies suggests that AVF is released by a rapid activation of a pre-existing precursor, thus supporting previous results.

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“…(a) TMV infectivity in leaf-discs of tobacco 'Samsun' treated with AVF or control preparation 15 min after inoculation. (b) As in (a) but AVF and control solutions were replaced with fresh solutions at 48 h. Otsuki, Shimomura & Takebe (1972) reported that the inhibition of TMV biosynthesis in a localizing host takes place between 24 anJ 48 h after inoculation. Essentially the same kinetics were observed by us (Fig.…”

Section: Inhibition Of Tmv Multiplication By a Vfmentioning

confidence: 99%

Further Studies on the Biology of an Antiviral Factor (AVF) From Virus-Infected Plants and Its Association With the N-Gene of Nicotiana Species

Antignus

Sela

Harpaz

1977

Journal of General Virology

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SUMMARYThe antiviral factor (AVF) from virus-infected plants, purified on polyacrylamide gels, could be labelled with radioactive phosphorus and its activity could be eluted from the gels. The radioactivity and the antiviral activity were co-purified and thus co-electropboresed; hence, the previously reported radioactive zone (Antignus, Sela & Harpaz, 1975) can be regarded as AVF. The production of AVF requires both the presence of the N-gene in the plant as well as virus infection. AVF production is inhibited by actinomycin D, but its activity is not affected by this drug. AVF production is correlated in time with the development of virus resistance in a local-lesion host. AVF inhibits TMV multiplication in infected leaves and suppresses virus synthesis almost totally in a local-lesion host. Some AVF is also produced when Nicotiana glutinosa L. is infected with a non-localized virus, but to a much lesser extent and at a later stage of infection. The production of AVF in N. glutinosa is not blocked at 30 o C, even though TMV is no longer localized at this temperature.

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Tobacco mosaic virus multiplication and expression of the N gene in necrotic responding tobacco varieties

Cited by 137 publications

References 16 publications

Antiviral Resistance Protein Tm-2² Functions on the Plasma Membrane

Antiviral Resistance Protein Tm-2² Functions on the Plasma Membrane

Polyinosinic{middle dot}Polycytidylic Acid in Association with Cyclic Nucleotides Activates the Antiviral Factor (AVF) in Plant Tissues

Further Studies on the Biology of an Antiviral Factor (AVF) From Virus-Infected Plants and Its Association With the N-Gene of Nicotiana Species

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Tobacco mosaic virus multiplication and expression of the N gene in necrotic responding tobacco varieties

Cited by 137 publications

References 16 publications

Antiviral Resistance Protein Tm-22 Functions on the Plasma Membrane

Antiviral Resistance Protein Tm-22 Functions on the Plasma Membrane

Polyinosinic{middle dot}Polycytidylic Acid in Association with Cyclic Nucleotides Activates the Antiviral Factor (AVF) in Plant Tissues

Further Studies on the Biology of an Antiviral Factor (AVF) From Virus-Infected Plants and Its Association With the N-Gene of Nicotiana Species

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Antiviral Resistance Protein Tm-2² Functions on the Plasma Membrane

Antiviral Resistance Protein Tm-2² Functions on the Plasma Membrane