Antiviral Resistance Protein Tm-2<sup>2</sup> Functions on the Plasma Membrane

Chen, Tianyuan; Liú, Dan; Niu, Xiaolin; Wang, Junzhu; Qian, Lichao; Han, Lu; Liu, Na; Zhao, Jinping; Hong, Yiguo; Liu, Yule

doi:10.1104/pp.16.01512

Cited by 56 publications

(76 citation statements)

References 83 publications

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“…LIC-pnLUC was generated by cloning LIC cassette containing ccdB gene flanking LIC adaptors with Apa I site (PCR amplified using pYL436 as a template) into pCAMBIA-nLUC [ 41 ]. C4 and C4 R13A were PCR amplified, and then cloned into LIC-pnLUC to C4-nLUC, C4 R13A -nLUC expression constructs by LIC method as described [ 59 ].…”

Section: Methodsmentioning

confidence: 99%

Cotton Leaf Curl Multan virus C4 protein suppresses both transcriptional and post-transcriptional gene silencing by interacting with SAM synthetase

et al. 2018

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Gene silencing is a natural antiviral defense mechanism in plants. For effective infection, plant viruses encode viral silencing suppressors to counter this plant antiviral response. The geminivirus-encoded C4 protein has been identified as a gene silencing suppressor, but the underlying mechanism of action has not been characterized. Here, we report that Cotton Leaf Curl Multan virus (CLCuMuV) C4 protein interacts with S-adenosyl methionine synthetase (SAMS), a core enzyme in the methyl cycle, and inhibits SAMS enzymatic activity. By contrast, an R13A mutation in C4 abolished its capacity to interact with SAMS and to suppress SAMS enzymatic activity. Overexpression of wild-type C4, but not mutant C4R13A, suppresses both transcriptional gene silencing (TGS) and post-transcriptional gene silencing (PTGS). Plants infected with CLCuMuV carrying C4R13A show decreased levels of symptoms and viral DNA accumulation associated with enhanced viral DNA methylation. Furthermore, silencing of NbSAMS2 reduces both TGS and PTGS, but enhanced plant susceptibility to two geminiviruses CLCuMuV and Tomato yellow leaf curl China virus. These data suggest that CLCuMuV C4 suppresses both TGS and PTGS by inhibiting SAMS activity to enhance CLCuMuV infection in plants.

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Section: Methodsmentioning

confidence: 99%

Cotton Leaf Curl Multan virus C4 protein suppresses both transcriptional and post-transcriptional gene silencing by interacting with SAM synthetase

et al. 2018

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“…Vectors HA-βC1, HA-cLUC, nYFP-βC1, and nLUC-βC1 were previously described (Haxim et al, 2017). HA-βC1 3A and other HA-βC1 mutations were obtained by overlapping PCR, and then cloned between the duplicated CaMV 35S promoter and the NOS terminator of pJG045, a pCAMBIA1300-based T-DNA vector (Chen et al, 2017). CLCuMuB-βC1 3A was generated by replacing CLCuMuB βC1 with βC1 3A by overlapping PCR.…”

Section: Plasmid Constructsmentioning

confidence: 99%

“…Cell fractionation and immunoblotting assays were conducted as described previously (Chen et al, 2017).…”

Section: Cell Fractionation Assaymentioning

confidence: 99%

Cotton leaf curl Multan virus βC1 Protein Induces Autophagy by Disrupting the Interaction of Autophagy-Related Protein 3 with Glyceraldehyde-3-Phosphate Dehydrogenases[OPEN]

et al. 2020

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Autophagy plays an important role in plant-pathogen interactions. Several pathogens including viruses induce autophagy in plants, but the underpinning mechanism remains largely unclear. Furthermore, in virusplant interactions, viral factor(s) that induce autophagy have yet to be identified. Here, we report that the βC1 protein of Cotton leaf curl Multan betasatellite (CLCuMuB) interacts with cytosolic glyceraldehyde-3phosphate dehydrogenase (GAPC), a negative autophagic regulator, to induce autophagy in Nicotiana benthamiana. CLCuMuB βC1 bound to GAPCs and disrupted the interaction between GAPCs and autophagyrelated protein 3 (ATG3). A mutant βC1 protein (βC1 3A) in which I45, Y48, and I53 were all substituted with alanine (A), had a dramatically reduced binding capacity with GAPCs, failed to disrupt the GAPCs-ATG3 interactions and failed to induce autophagy. Furthermore, mutant virus carrying βC1 3A showed increased symptoms and viral DNA accumulation associated with decreased autophagy in plants. These results suggest that CLCuMuB βC1 activates autophagy by disrupting GAPCs-ATG3 interactions.

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“…). Furthermore, Tm‐2 2 associates with MP at the plasma membrane (Chen et al , ) and recognition of MP by Tm‐2 2 may involve an intermediate MP Interacting Protein 1 (NbMIP1) (Du et al , ). However, evidence for the existence of Tm‐2 2 , MP and NbMIP1 in a complex is currently lacking.…”

Section: Introductionmentioning

confidence: 99%

Perspectives on intracellular perception of plant viruses

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Nagalakshmi

et al. 2019

Molecular Plant Pathology

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Summary The intracellular nucleotide‐binding domain leucine‐rich repeat (NLR) class of immune receptors plays an important role in plant viral defence. Plant NLRs recognize viruses through direct or indirect association of viral proteins, triggering a downstream defence response to prevent viral proliferation and movement within the plant. This review focuses on current knowledge of intracellular perception of viral pathogens, activation of NLRs and the downstream signalling components involved in plant viral defence.

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Antiviral Resistance Protein Tm-2² Functions on the Plasma Membrane

Cited by 56 publications

References 83 publications

Cotton Leaf Curl Multan virus C4 protein suppresses both transcriptional and post-transcriptional gene silencing by interacting with SAM synthetase

Cotton Leaf Curl Multan virus C4 protein suppresses both transcriptional and post-transcriptional gene silencing by interacting with SAM synthetase

Cotton leaf curl Multan virus βC1 Protein Induces Autophagy by Disrupting the Interaction of Autophagy-Related Protein 3 with Glyceraldehyde-3-Phosphate Dehydrogenases[OPEN]

Perspectives on intracellular perception of plant viruses

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Antiviral Resistance Protein Tm-22 Functions on the Plasma Membrane

Cited by 56 publications

References 83 publications

Cotton Leaf Curl Multan virus C4 protein suppresses both transcriptional and post-transcriptional gene silencing by interacting with SAM synthetase

Cotton Leaf Curl Multan virus C4 protein suppresses both transcriptional and post-transcriptional gene silencing by interacting with SAM synthetase

Cotton leaf curl Multan virus βC1 Protein Induces Autophagy by Disrupting the Interaction of Autophagy-Related Protein 3 with Glyceraldehyde-3-Phosphate Dehydrogenases[OPEN]

Perspectives on intracellular perception of plant viruses

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Antiviral Resistance Protein Tm-2² Functions on the Plasma Membrane