To Heal or Not to Heal: Predictive Value of In Vivo Reflectance-Mode Confocal Microscopy in Assessing Healing Course of Human Burn Wounds

Altıntaş, Ahmet; Guggenheim, Merlin; Altintas, M.A.; Amini, Peymaneh; Stasch, Tilman; Spilker, G.

doi:10.1097/bcr.0b013e3181bfb810

Cited by 6 publications

(7 citation statements)

References 19 publications

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“…9 Among them, in vivo reflectance confocal laser scanning microscopy (CLSM) represents an innovative optical imaging tool for noninvasive evaluation of normal and diseased skin in vivo and in real time. 11,12 Their findings supported the use of CLSM as an adjunctive tool for evaluation and management of burn wounds. 10 Altintas and co-workers have first employed CLSM for evaluation of burn wounds in human volunteers, whereby aspects of microcirculation, inflammation, and histomorphology have been described.…”

Section: Introductionmentioning

confidence: 71%

Applicability of confocal laser scanning microscopy for evaluation and monitoring of cutaneous wound healing

et al. 2012

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There is a high demand for noninvasive imaging techniques for wound assessment. In vivo reflectance confocal laser scanning microscopy (CLSM) represents an innovative optical technique for noninvasive evaluation of normal and diseased skin in vivo at near cellular resolution. This study was designed to test the feasibility of CLSM for noninvasive analysis of cutaneous wound healing in 15 patients (7 male/8 female), including acute and chronic, superficial and deep dermal skin wounds. A commercially available CLSM system was used for the assessment of wound bed and wound margins in order to obtain descriptive cellular and morphological parameters of cutaneous wound repair noninvasively and over time. CLSM was able to visualize features of cutaneous wound repair in epidermal and superficial dermal wounds, including aspects of inflammation, neovascularisation, and tissue remodelling in vivo. Limitations include the lack of mechanic fixation of the optical system on moist surfaces restricting the analysis of chronic skin wounds to the wound margins, as well as a limited optical resolution in areas of significant slough formation. By describing CLSM features of cutaneous inflammation, vascularisation, and epithelialisation, the findings of this study support the role of CLSM in modern wound research and management.

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Section: Introductionmentioning

confidence: 71%

Applicability of confocal laser scanning microscopy for evaluation and monitoring of cutaneous wound healing

et al. 2012

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“…Moreover the thickness of the basal layer was preserved in the healing group compared to the obliteration of the basal layer in the nonhealing group at 36 h of measurement. The preliminary study showed some important findings and paved way for further research [55]. …”

Section: Discussionmentioning

confidence: 99%

A Systematic Review of the Evolution of Laser Doppler Techniques in Burn Depth Assessment

Khatib

Jabir

O’Connor

et al. 2014

Plastic Surgery International

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Aims. The introduction of laser Doppler (LD) techniques to assess burn depth has revolutionized the treatment of burns of indeterminate depth. This paper will systematically review studies related to these two techniques and trace their evolution. At the same time we hope to highlight current controversies and areas where further research is necessary with regard to LD imaging (LDI) techniques. Methods. A systematic search for relevant literature was carried out on PubMed, Medline, EMBASE, and Google Scholar. Key search terms included the following: “Laser Doppler imaging,” “laser Doppler flow,” and “burn depth.” Results. A total of 53 studies were identified. Twenty-six studies which met the inclusion/exclusion criteria were included in the review. Conclusions. The numerous advantages of LDI over those of LD flowmetry have resulted in the former technique superseding the latter one. Despite the presence of alternative burn depth assessment techniques, LDI remains the most favoured. Various newer LDI machines with increasingly sophisticated methods of assessing burn depth have been introduced throughout the years. However, factors such as cost effectiveness, scanning of topographically inconsistent areas of the body, and skewing of results due to tattoos, peripheral vascular disease, and anaemia continue to be sighted as obstacles to LDI which require further research.

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“…Confocal microscopy or confocal laser scanning microscopy (CLSM) is a high‐resolution optical detection technique, which provides the ability to monitor specimens in multiple planes of depth by adjusting a specific focal depth . In confocal microscopy, a point light source is focused onto a very small volume of a sample.…”

Section: Microscopymentioning

confidence: 99%

Noninvasive imaging technologies for cutaneous wound assessment: A review

Paul

Ghassemi

Ramella‐Roman

et al. 2015

Wound Repair Regeneration

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The ability to phenotype wounds for the purposes of assessing severity, healing potential and treatment is an important function of evidence-based medicine. A variety of optical technologies are currently in development for noninvasive wound assessment. To varying extents, these optical technologies have the potential to supplement traditional clinical wound evaluation and research, by providing detailed information regarding skin components imperceptible to visual inspection. These assessments are achieved through quantitative optical analysis of tissue characteristics including blood flow, collagen remodeling, hemoglobin content, inflammation, temperature, vascular structure, and water content. Technologies that have, to this date, been applied to wound assessment include: near infrared imaging, thermal imaging, optical coherence tomography, orthogonal polarization spectral imaging, fluorescence imaging, laser Doppler imaging, microscopy, spatial frequency domain imaging, photoacoustic detection, and spectral/hyperspectral imaging. We present a review of the technologies in use or development for these purposes with three aims: (1) providing basic explanations of imaging technology concepts, (2) reviewing the wound imaging literature, and (3) providing insight into areas for further application and exploration. Noninvasive imaging is a promising advancement in wound assessment and all technologies require further validation.

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To Heal or Not to Heal: Predictive Value of In Vivo Reflectance-Mode Confocal Microscopy in Assessing Healing Course of Human Burn Wounds

Cited by 6 publications

References 19 publications

Applicability of confocal laser scanning microscopy for evaluation and monitoring of cutaneous wound healing

Applicability of confocal laser scanning microscopy for evaluation and monitoring of cutaneous wound healing

A Systematic Review of the Evolution of Laser Doppler Techniques in Burn Depth Assessment

Noninvasive imaging technologies for cutaneous wound assessment: A review

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