Applicability of confocal laser scanning microscopy for evaluation and monitoring of cutaneous wound healing

Lange‐Asschenfeldt, Susanne; Боб, А. О.; Terhorst‐Molawi, Dorothea; Ulrich, Martina; Fluhr, Joachim W.; Mendez, Gil; Roewert-Huber, Hans-Joachim; Stockfleth, Eggert; Lange‐Asschenfeldt, Bernhard

doi:10.1117/1.jbo.17.7.076016

Cited by 30 publications

(30 citation statements)

References 38 publications

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“…So far, histological analysis, including tissue sectioning and staining, is still considered the gold standard method for the morphological assessment of wounds [13,14]. Despite the standardized use of histological analysis methods, there are considerable drawbacks, which limit their applicability in wound characterization.…”

Section: Resultsmentioning

confidence: 99%

“…For reliable and reproducible analytical evaluation of wound samples, there is a strong need for an alternative technology. Here, optical imaging methods have been gaining relevance as suitable tools for noninvasive wound analysis [13,14,15,16,17,18]. In this context, optical profilometry represents a novel technical possibility for nondestructive and label-free monitoring of wound topography.…”

Section: Introductionmentioning

confidence: 99%

“…The analytical characterization of wounds is mostly performed by histological tissue sectioning and staining [13,14]. Despite the widespread use of this experimental procedure, it encounters significant drawbacks such as the destruction of the sample and the limitation to one time point analysis.…”

Section: Introductionmentioning

confidence: 99%

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Novel in vitro Approaches for the Simulation and Analysis of Human Skin Wounds

Planz

Franzen

Windbergs

2014

Skin Pharmacol Physiol

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Considering the increasing incidence of chronic wounds and severe wound infections, effective drug delivery to wounded skin is of high importance. The rational development of novel therapeutic systems requires appropriate in vitro testing methodologies. In this context, suitable and reliable in vitro models simulating human wounds and advanced analytical techniques for precise wound characterization are urgently needed. In this study, we introduce a novel in vitro model based on excised human skin. In contrast to the established wound models, our novel approach has a coffin-shaped, linear, rectangular geometry with defined wound edges exhibiting consistent appearance along the entire wound bed. In addition, we introduce optical profilometry as a novel technique for nondestructive wound analysis. We successfully demonstrate the applicability of this optical imaging method based on white light reflection for three-dimensional visualization of different wound models. Furthermore, we create virtual noninvasive cross sections of these wounds to assess wound geometry in direct comparison to conventional histological analysis. Imaging analysis of our novel coffin-shaped model resulted in reproducible virtual sections along the entire wound bed. Our findings indicate the potential of our novel in vitro model for improved simulation of human wounds. Further, we successfully overcome the limitations of conventional histological analysis by the employment of optical profilometry for nondestructive three-dimensional wound characterization. © 2014 S. Karger AG, Basel

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Novel in vitro Approaches for the Simulation and Analysis of Human Skin Wounds

Planz

Franzen

Windbergs

2014

Skin Pharmacol Physiol

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“…Lange-Asschenfeld 41 and Caspers 42 showed confocal laser-scanning microscopes for in vivo imaging of human skin morphology and wound healing. Both studies mention the potential use for pharmaceutical research on drug delivery.…”

Section: Feasibility For Clinical In Vivo Applicationsmentioning

confidence: 99%

Evaluation and quantification of spectral information in tissue by confocal microscopy

Maeder¹,

Marquardt²,

Beer³

et al. 2012

J. Biomed. Opt

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Abstract. A confocal imaging and image processing scheme is introduced to visualize and evaluate the spatial distribution of spectral information in tissue. The image data are recorded using a confocal laser-scanning microscope equipped with a detection unit that provides high spectral resolution. The processing scheme is based on spectral data, is less error-prone than intensity-based visualization and evaluation methods, and provides quantitative information on the composition of the sample. The method is tested and validated in the context of the development of dermal drug delivery systems, introducing a quantitative uptake indicator to compare the performances of different delivery systems is introduced. A drug penetration study was performed in vitro. The results show that the method is able to detect, visualize and measure spectral information in tissue. In the penetration study, uptake efficiencies of different experiment setups could be discriminated and quantitatively described. The developed uptake indicator is a step towards a quantitative assessment and, in a more general view apart from pharmaceutical research, provides valuable information on tissue composition. It can potentially be used for clinical in vitro and in vivo applications.

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“…Recently, in vivo reflectance CLSM has been used for noninvasive evaluation of normal and diseased human skin near the limits of cellular resolution, although there are some limitations on fixation of the optical system on moist surfaces. 3 Reflectance CLSM has been used in the quantification of epidermal pigmentation and dermal papilla density. 4 A technique that is derived from CLSM and applied to skin research is multi-photon laser scanning microscopy (MPLSM).…”

Section: Confocal Microscopy and Its Application In Skinmentioning

confidence: 99%