TNF-α-induced increase in intestinal epithelial tight junction permeability requires NF-κB activation

Thomas, Y.; Iwamoto, Gary K.; Hoa, Neil; Akotia, Vimesh; Pedram, Ali; Boivin, Michel; Said, Hamid M.

doi:10.1152/ajpgi.00173.2003

Cited by 774 publications

(733 citation statements)

References 58 publications

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“…In human vascular endothelial cells, IL-8 can down-regulate the gene expressions of occludin [72]. In addition, TNF-a can decrease ZO-1 gene expression in Caco-2 cells [73] and claudin 3 in human endothelial cells [74]. In the present study, the effects of Phe on IL-8 and TNF-a gene expression showed the opposite tendency with the gene expression of claudin 3, occludin and ZO-1.…”

Section: Phe Regulated Tj Protein Transcript Abundance In the Intesticontrasting

confidence: 43%

Dietary phenylalanine-improved intestinal barrier health in young grass carp (Ctenopharyngodon idella) is associated with increased immune status and regulated gene expression of cytokines, tight junction proteins, antioxidant enzymes and related signalling molecules

Feng

Wen

Liu

et al. 2015

Fish & Shellfish Immunology

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Section: Phe Regulated Tj Protein Transcript Abundance In the Intesticontrasting

confidence: 43%

Dietary phenylalanine-improved intestinal barrier health in young grass carp (Ctenopharyngodon idella) is associated with increased immune status and regulated gene expression of cytokines, tight junction proteins, antioxidant enzymes and related signalling molecules

Feng

Wen

Liu

et al. 2015

Fish & Shellfish Immunology

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“…It was reported that pro-inflammatory cytokines namely TNF-a and IL-8 have been found to be involved in regulating TJ protein expression [75]. In Caco-2 cells, studies showed that TNFa down-regulated the expression of ZO-1 [76] and Occludin [77] respectively. Moreover, IL-8 decreased the mRNA level of Occludin, Claudin-5 and ZO-1 in human [78].…”

Section: Valine Regulated Tj Protein Transcript Abundance In the Intementioning

confidence: 99%

The impaired intestinal mucosal immune system by valine deficiency for young grass carp (Ctenopharyngodon idella) is associated with decreasing immune status and regulating tight junction proteins transcript abundance in the intestine

Luo

Feng

Jiang³

et al. 2014

Fish & Shellfish Immunology

151

113

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“…Tight junction leakiness has been observed in preneoplastic growths as well as actual adenocarcinomas (Soler et al, 1999). Structural alteration of tight junctions in tumors of various tissues has likewise been reported (MartinezPalomo, 1970;Polak-Charcon et al, 1980;Robenek et al, 1981;Swift et al, 1983;Ma et al, 2004). In addition, there has been extensive documentation of the tight junction leakiness induced by tumor-promoting cocarcinogenic chemicals, most notably phorbol esters such as 12-O-tetradecanoylphorbol-13-acetate (TPA) and phorbol-12,13-dibutyrate (Mullin and O'Brien, 1986;Mullin et al, 1990).…”

mentioning

confidence: 93%

RasMutation Impairs Epithelial Barrier Function to a Wide Range of Nonelectrolytes

Mullin

Leatherman

Valenzano

et al. 2005

MBoC

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Although ras mutations have been shown to affect epithelial architecture and polarity, their role in altering tight junctions remains unclear. Transfection of a valine-12 mutated ras construct into LLC-PK 1 renal epithelia produces leakiness of tight junctions to certain types of solutes. Transepithelial permeability of D-mannitol increases sixfold but transepithelial electrical resistance increases >40%. This indicates decreased paracellular permeability to NaCl but increased permeability to nonelectrolytes. Permeability increases to D-mannitol (M r 182), polyethylene glycol (M r 4000), and 10,000-M r methylated dextran but not to 2,000,000-M r methylated dextran. This implies a "ceiling" on the size of solutes that can cross a ras-mutated epithelial barrier and therefore that the increased permeability is not due to loss of cells or junctions. Although the abundance of claudin-2 declined to undetectable levels in the ras-overexpressing cells compared with vector controls, levels of occludin and claudins 1, 4, and 7 increased. The abundance of claudins-3 and -5 remained unchanged. An increase in extracellular signal-regulated kinase-2 phosphorylation suggests that the downstream effects on the tight junction may be due to changes in the mitogen-activated protein kinase signaling pathway. These selective changes in permeability may influence tumorigenesis by the types of solutes now able to cross the epithelial barrier. INTRODUCTIONMutations in the three closely related ras genes, H-ras, K-ras, and N-ras, are among the most common mutations found in human cancer, reaching 50% in some types of tumors, such as colorectal carcinoma (Forrester et al., 1987;Andreyev et al., 1997Andreyev et al., , 1998Andreyev et al., , 2001). Vogelstein and colleagues have shown that mutations in K-ras occur early in the development of colon carcinomas (Vogelstein et al., 1988). This dominantly acting mutation in ras results in its constitutive activation and the subsequent triggering of its signaling pathway by influencing GTPase activity. It is clear that mutations in ras contribute both to the recurrence of the disease and to decreased survival (Andreyev et al., 1998). It is less clear, however, what the consequences of ras mutations are during the early stages of tumorigenesis. In this regard, one important issue is the effect of Ras activation on epithelial barrier function, perhaps the most basic of all epithelial functions, and a function shared by all epithelial tissues. Ras can exert fundamental effects on epithelial barrier function through the extracellular signal-regulated kinase-mitogen-activated protein (ERK-MAP) kinase pathway or through direct binding to the tight junction-associated protein, AF-6, which binds to ZO-1, which in turn links the tight junction to the actin cytoskeleton. Activated Ras is known to inhibit interaction between the tight junction-associated proteins, AF-6 and ZO-1, with resultant perturbation of intercellular junctions (Yamamoto et al., 1997). Modulation of tight junctions by G proteins in ge...

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TNF-α-induced increase in intestinal epithelial tight junction permeability requires NF-κB activation

Cited by 774 publications

References 58 publications

Dietary phenylalanine-improved intestinal barrier health in young grass carp (Ctenopharyngodon idella) is associated with increased immune status and regulated gene expression of cytokines, tight junction proteins, antioxidant enzymes and related signalling molecules

Dietary phenylalanine-improved intestinal barrier health in young grass carp (Ctenopharyngodon idella) is associated with increased immune status and regulated gene expression of cytokines, tight junction proteins, antioxidant enzymes and related signalling molecules

The impaired intestinal mucosal immune system by valine deficiency for young grass carp (Ctenopharyngodon idella) is associated with decreasing immune status and regulating tight junction proteins transcript abundance in the intestine

RasMutation Impairs Epithelial Barrier Function to a Wide Range of Nonelectrolytes

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