TNF‐α hyperpolarizes membrane potential and potentiates the response to nicotinic receptor stimulation in cultured rat myenteric neurones

Rehn, Matthias; Hübschle, Thomas; Diener, Martin

doi:10.1111/j.1365-201x.2004.01269.x

Cited by 31 publications

(28 citation statements)

References 45 publications

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“…TNF-α plays an important role in IBD [30]. The TNF/TNFR signaling system mediates mucosal damage through the enhancement of NF-ĸB activity by continuous infiltration of TNF-producing cells, probably a key pathogeneses of colitis [31].…”

Section: Discussionmentioning

confidence: 99%

Effect of Passive Cigarette Smoking on Colonic α7-Nicotinic Acetylcholine Receptors in TNBS-Induced Colitis in Rats

Sun¹,

Wang²,

et al. 2007

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Background and Aims: Cigarette smoking affects colonic inflammation, but the exact mechanism by which it does so is unclear. The aim of this study was to investigate the underlying mechanism by examining the effect of cigarette smoking on 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis in rats. Method: Experimental colitis was induced by administrating TNBS enema in Sprague-Dawley rats. The effect of cigarette smoking was assessed by measuring the colonic edema, mucosal lesion area, histopathological score, mucosal myeloperoxidase (MPO) activity and tumor necrosis factor-α (TNF-α). The expression of α7-nicotinic acetylcholine receptor (α7nAChR) was examined after cigarette smoking to identify whether the α7nAChR is involved in inflammation. Results: TNBS induced severe colitis as evidenced by increased colonic edema, mucosal lesion area, histopathological score, MPO activity, and TNF-α level. Inflammation was aggravated by cigarette smoke exposure. Colonic tissue expressed α7nAChR (0.41 ± 0.11), and TNBS up-regulated the receptor expression (0.46 ± 0.11), but the difference was not significant (p > 0.05). Cigarette smoking with 2 and 4% respectively significantly increased the expression of α7nAChR (0.55 ± 0.05 and 0.64 ± 0.08) (p < 0.05). Conclusions: The up-regulated expression of α7nAChR after exposure to cigarette smoking indicates that α7nAChR in colonic tissue may be involved in cigarette smoking damage in TNBS-induced colitis in rats.

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Section: Discussionmentioning

confidence: 99%

Effect of Passive Cigarette Smoking on Colonic α7-Nicotinic Acetylcholine Receptors in TNBS-Induced Colitis in Rats

Sun¹,

Wang²,

et al. 2007

Digestion

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“…Furthermore, in a recent study at cultured myenteric ganglia we observed that pre-treatment with TNF-a over night hyperpolarised the membrane potential and potentiated the depolarisation evoked by nicotinic receptor stimulation (Rehn et al, 2004). Thus in the present study we were interested in possible changes of [Ca 2+ ] i after nicotinic receptor stimulation in the presence of this cytokine.…”

Section: Do Mediators Of Inflammation Affect [Ca 2+ ] I Of Myenteric mentioning

confidence: 90%

“…depolarisation evoked by carbachol, which predominantly stimulates nicotinergic receptors at the myenteric ganglia (Rehn et al, 2004). Nifedipine (10 À 5 mol l À 1 ) and CgTx-GVIA (10 À 6 mol l À 1 ) both reduced the increase of the fura-2 fluorescence ratio evoked by carbachol (5 Â 10 À 5 mol l À 1 ; Fig.…”

Section: Response To Cholinergic Receptor Stimulationmentioning

confidence: 97%

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Pharmacological characterisation of voltage-dependent Ca2+ channels in isolated ganglia from the myenteric plexus

Schäufele

Diener

2005

Life Sciences

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“…TNF-␣ also modulates synaptic maturation and neuronal branching in the hippocampus by controlling the expression of synaptic vesicle-associated proteins [84] . Moreover, TNF-␣ changes the electrophysiological properties of myenteric neurons via cyclooxygenase metabolites and protein tyrosine phosphorylation [85] .…”

Section: Tnf-␣mentioning

confidence: 99%

The Role of Cytokines in the Regulation of Neurotransmission

Camacho‐Arroyo

López-Griego

Morales‐Montor

2008

Neuroimmunomodulation

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Cytokines are highly inducible, secretory proteins that mediate intercellular communication in the immune system. They are grouped in several protein families, namely tumor necrosis factors, interleukins, interferons and colony-stimulating factors. In recent years, evidence has elucidated that some of these proteins as well as their receptors are also produced in the central nervous system (CNS) by specific neural cell lineages under physiological and pathological conditions. Cytokines regulate a variety of processes in the CNS, including neurotransmission. The current data let us to suggest that cytokines play an important role in the regulation of both excitatory and inhibitory neurotransmission in the CNS. This knowledge could be fundamental for the proposal of new therapeutic approaches to neurological and psychiatric disorders.

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TNF‐α hyperpolarizes membrane potential and potentiates the response to nicotinic receptor stimulation in cultured rat myenteric neurones

Cited by 31 publications

References 45 publications

Effect of Passive Cigarette Smoking on Colonic α7-Nicotinic Acetylcholine Receptors in TNBS-Induced Colitis in Rats

Effect of Passive Cigarette Smoking on Colonic α7-Nicotinic Acetylcholine Receptors in TNBS-Induced Colitis in Rats

Pharmacological characterisation of voltage-dependent Ca2+ channels in isolated ganglia from the myenteric plexus

The Role of Cytokines in the Regulation of Neurotransmission

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