TNF-α and IL-6 in the Diagnosis of Bacterial and Aseptic Meningitis in Children

Mukai, Adriana O.; Krebs, Vera Lúcia Jornada; Bertoli, Ciro João; Okay, Thelma Suely

doi:10.1016/j.pediatrneurol.2005.06.003

Cited by 62 publications

(44 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Sheep had CSF TNFa, IL-12(p40) and Il-6 levels >80.7, 117.5 and 490.5 pg/mL, respectively were more likely to die. This confirms the deleterious effect of such cytokines, already reported in other species in multiple studies [10,13,14,[24][25][26][27][28].…”

Section: Discussionsupporting

confidence: 90%

Tumor Necrosis Factor-α, Interleukins-12(p40), 6, and 10 levels in cerebrospinal fluid and outcome prediction in Ossimi sheep with encephalitic listeriosis

et al. 2015

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 90%

Tumor Necrosis Factor-α, Interleukins-12(p40), 6, and 10 levels in cerebrospinal fluid and outcome prediction in Ossimi sheep with encephalitic listeriosis

et al. 2015

View full text Add to dashboard Cite

“…When bacterial products such as lipopolysaccharide, teichoic acid, or peptidoglycan are introduced into the cerebrospinal fluid either by direct injection into the cerebrospinal fluid or by lysis of live bacteria with the administration of antibiotics, cytokines can be detected in the cerebrospinal fluid. (Mukai et al 2005). These data may provide some of explanations for our observation that there were high level of CSF and plasma B7-H3 only in our bacterial meningitis patients, where either LPS, teichoic acid, or peptidoglycan was the main nosogenetic factor.…”

Section: Discussionmentioning

confidence: 53%

Circulating B7-H3(CD276) Elevations in Cerebrospinal Fluid and Plasma of Children with Bacterial Meningitis

Chen

Zhang²,

et al. 2008

J Mol Neurosci

View full text Add to dashboard Cite

The objective of this study was to analyze the clinical significance of cerebrospinal fluid (CSF) and plasma concentrations of B7-H3, tumor necrosis factor-alpha (TNF-alpha), gamma interferon (IFN-gamma), and interleukin-17 (IL-17) in bacterial and aseptic meningitis in children. The participants were six children with bacterial meningitis, 16 with aseptic meningitis, and 12 control subjects. All participants were between 2 months and 12 years of age on admission. Cytokines determination was performed by enzyme-linked immunosorbent assay technique. CSF and plasma-circulating B7-H3 were significantly higher in the bacterial meningitis group as compared with the aseptic group (p = 0.001) and the control group (p = 0.000 and p = 0.001 respectively). However, CSF and plasma-circulating B7-H3 in aseptic meningitis were not significantly higher than control group (p = 0.071 and p = 0.72 respectively).CSF and plasma-circulating TNF-alpha were significantly higher in the bacterial meningitis group as compared with the aseptic group (p = 0.004 and p < 0.0001 respectively) and control group (p = 0.004 and p < 0.0001 respectively). Similarly, we did not observe significant elevated TNF-alpha levels in CSF and plasma in aseptic group compared with control group (p = 0.03 and p = 0.12 respectively). IFN-gamma levels in CSF and plasma were undetectable in control group, and we did not find statistical significances in both of CSF and plasma between the elevated IFN-gamma level in bacterial meningitis group and aseptic meningitis group(p = 0.055 and p = 0.095 respectively) CSF and plasma levels of IL-17 were undetectable in all subjects. There were correlations between B7-H3 and TNF-alpha, IFN-gamma (r = 0.875, p = 0.000; r = -0.693, p = 0.000, respectively) in CSF in meningitis subjects. In plasma, levels of B7-H3 in bacterial meningitis on admission correlated positively with TNF-alpha (r = 0.968, p = 0.002), and white blood cell counts (r = 0.973, p = 0.001). Detectable CSF levels of B7-H3, TNF-alpha, and IFN-gamma on admission were not associated significantly with any of CSF characteristics. Additionally, CSF and plasma levels of B7-H3 decreased remarkably after treatment. Altogether, our data indicated that circulating B7-H3 and TNF-alpha levels in the CSF and plasma were useful markers for distinguishing bacterial from aseptic meningitis, and Circulating B7-H3 was demonstrated to be useful in evaluating the intensity of the infectious inflammatory process in the central nervous system in children.

show abstract

“…Since leukocytes themselves may cause injury by releasing potentially harmful products such as free radicals, metalloproteases, perchloric acid, and eicosanoids, interactions between leukocytes and endothelial cells may also contribute to CNS damage during bacterial meningitis [11,34]. IL-6, a key mediator of the inflammatory response in swine infections [13], is considered to be one of the best markers of meningitis [12,27]. IL-6 release could also lead to an increase in BBB permeability, since this cytokine has been shown to modify transendothelial electrical resistance of cerebral endothelial cells, thereby reflecting alterations of BBB tight junctions [22].…”

Section: Discussionmentioning

confidence: 99%

Interactions ofHaemophilus parasuisand its LOS with porcine brain microvascular endothelial cells

et al. 2008

View full text Add to dashboard Cite

-Haemophilus parasuis is a swine pathogen that causes Glässer's disease, which is characterized by polyserositis and meningitis. The pathogenesis of the H. parasuis infection is poorly understood. To cause meningitis, H. parasuis has to cross the blood-brain barrier (BBB) to gain access to the central nervous system (CNS). We recently showed that H. parasuis adheres to and invades porcine brain microvascular endothelial cells (PBMEC). The aim of this study was to evaluate the role of H. parasuis lipooligosaccharide (LOS) in the adhesion to PBMEC and to determine if H. parasuis (and/or its LOS) is able to induce apoptosis and activation of PBMEC. Results showed that adhesion of H. parasuis to PBMEC was partially mediated by LOS. Moreover, H. parasuis induces caspase-3-mediated apoptosis of PBMEC in a time -and dosedependent manner, but its LOS did not seem to be involved in such a process. Furthermore, H. parasuis and, to a lesser extent, its LOS, was able to induce the release of IL-8 and IL-6 by PBMEC. Field strains of H. parasuis serotypes 4 and 5 induced similar levels of these inflammatory mediators. Our data suggest that H. parasuis uses cellular adhesion, induction of apoptosis and up-regulation of inflammatory mediators as mechanisms to invade the CNS via the BBB, and that LOS would play a certain but limited role in such pathological process. Haemophilus parasuis / LOS / endothelial cells / BBB / meningitis

show abstract

TNF-α and IL-6 in the Diagnosis of Bacterial and Aseptic Meningitis in Children

Cited by 62 publications

References 25 publications

Tumor Necrosis Factor-α, Interleukins-12(p40), 6, and 10 levels in cerebrospinal fluid and outcome prediction in Ossimi sheep with encephalitic listeriosis

Tumor Necrosis Factor-α, Interleukins-12(p40), 6, and 10 levels in cerebrospinal fluid and outcome prediction in Ossimi sheep with encephalitic listeriosis

Circulating B7-H3(CD276) Elevations in Cerebrospinal Fluid and Plasma of Children with Bacterial Meningitis

Interactions ofHaemophilus parasuisand its LOS with porcine brain microvascular endothelial cells

Contact Info

Product

Resources

About