Tn1546 structures and multilocus sequence typing of vanA-containing enterococci of animal, human and food origin

López, María José; Sáenz, Yolanda; Álvarez-Martínez, Míriam J.; Marco, Francesc; Robredo, Beatriz; Rojo–Bezares, Beatriz; Ruiz-Larrea, Fernanda; Zarazaga, Myriam; Torres, Carmén

doi:10.1093/jac/dkq192

Cited by 35 publications

(27 citation statements)

References 12 publications

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“…Moreover, this gene is usually located in transposon Tn1546 which facilities its mobilization and transference to other microorganisms. Our isolate showed identical Tn1546 structure to one previously described in two studies carried out in Spain, one of them in an E. faecium isolate from a wild black rat [13] and the other one in a clinical isolate [19]. This transposon and other resistance genes are often mobilized by the same plasmid, being transferred at the same time when one of the antimicrobial agents is used.…”

Section: Discussionsupporting

confidence: 67%

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Characterization of fecal vancomycin-resistant enterococci with acquired and intrinsic resistance mechanisms in wild animals, Spain

Lozano¹,

González‐Barrio

Camacho

et al. 2015

Microb Ecol

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The objectives were to evaluate the presence of vancomycin-resistant enterococci with acquired (VRE-a) and intrinsic (VRE-i) resistance mechanisms in fecal samples from different wild animals, and analyze their phenotypes and genotypes of antimicrobial resistance. A total of 348 cloacal/rectal samples from red-legged partridges (127), white storks (81), red kites (59), and wild boars (81) (June 2014/February 2015) were inoculated in Slanetz-Bartley agar supplemented with vancomycin (4 μg/mL). We investigated the susceptibility to 12 antimicrobials and the presence of 19 antimicrobial resistance and five virulence genes. In addition, we performed multilocus sequence typing, detection of IS16 and studied Tn1546 structure. One VRE-a isolate was identified in one wild boar. This isolate was identified as Enterococcus faecium, harbored vanA gene included into Tn1546 (truncated with IS1542/IS1216), and belonged to the new ST993. This isolate contained the erm(A), erm(B), tet(M), dfrG, and dfrK genes. Neither element IS16 nor the studied virulence genes were detected. Ninety-six VRE-i isolates were identified (89 Enterococcus gallinarum and seven Enterococcus casseliflavus), with the following prevalence: red kites (71.2 %), white storks (46.9 %), red-legged partridges (7.9 %), and wild boars (4.9 %). Most E. gallinarum isolates showed resistance to tetracycline (66.3 %) and/or erythromycin (46.1 %). High-level resistance to aminoglycosides was present among our VRE-i isolates: kanamycin (22.9 %), streptomycin (11.5 %), and gentamicin (9.4 %). In general, VRE-i isolates of red kites showed higher rates of resistance for non-glycopeptide agents than those of other animal species. The dissemination of acquired resistance mechanisms in natural environments could have implications in the global spread of resistance with public health implications.

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Section: Discussionsupporting

confidence: 67%

“…The structure of the transposon Tn1546, in the case of vanA isolates, was determined thanks to the use of specific primers and the sequencing of the obtained amplicons (Table 1) [19]. …”

Section: Vancomycin Resistance Mechanismsmentioning

confidence: 99%

Characterization of fecal vancomycin-resistant enterococci with acquired and intrinsic resistance mechanisms in wild animals, Spain

Lozano¹,

González‐Barrio

Camacho

et al. 2015

Microb Ecol

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“…This gene could have a great role in the exchanging genetic mobile elements and could also be implicated in the adaptation of these bacteria to the hospital environment [29,30].Remarkably, our vanA E. faecium isolates presented a multiresistance phenotype, being resistant, in addition to glycopeptides, to erythromycin, tetracycline, ciprofloxacin, ampicillin, kanamycin, streptomycin, pristinamycin, and trimethoprimsulfamethoxazole. In the case of resistance genes erm(B) and tet(M), it is interesting to mention that they have been previously detected in the same conjugative plasmid as the van(A) gene, which indicates that coselection processes might have happened [31]. Our VRE strains were still susceptible to high dose of gentamicin, similar to what was found in a previous study reported in Tunisia showing the emergence of epidemic CC17 clones of vanA E. faecium that were also sensitive to high dose of gentamicin [6].…”

Section: Discussionmentioning

confidence: 99%

Multidrug-resistant enterococci in the hospital environment: detection of novel vancomycin-resistant E. faecium clone ST910

Dziri

Lozano

Saïd

et al. 2016

J Infect Dev Ctries

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Introduction: The role of the hospital environment as a reservoir of resistant bacteria in Tunisia has been poorly investigated; however, it could be responsible for the transmission of multidrug-resistant bacteria. The objective was to study the prevalence of Enterococcus in the environment of a Tunisian hospital and the antibiotic resistance phenotype/genotype in recovered isolates, with special reference to vancomycin resistance. Methodology: A total of 300 samples were taken (March-June, 2013) and inoculated in Slanetz-Bartley agar plates supplemented or not supplemented with 8 µg/mL of vancomycin. Antibiotic resistance genes were tested by polymerase chain reaction (PCR). The clonal relatedness of the vanA isolates was assessed using pulsed-field gel electrophoresis (PFGE) and multilocus sequence testing (MLST). Results: Enterococci were recovered in 33.3% of tested samples inoculated in SB medium. E faecium was the most prevalent species, followed by E. faecalis and E. casseliflavus. Antimicrobial resistance genes detected were as follows (number of isolates): erm(B) (71), tet(M) (18), aph(3')-IIIa (27), ant(6)-Ia (15), cat(A) (4), and van(C2) (6). Vancomycin-resistant-enterococci (VRE) were recovered from 14 samples (4.7%), when tested in SB-VAN. The 14 VRE (one per positive sample) were identified as E. faecium and contained the van(A),erm(B), tet(M), ant(6)-Ia, and aph(3')-IIIa genes. Thirteen of the VRE strains were ascribed by PFGE and MLST to a novel clone (new ST910), and only one VRE strain was typed as ST80 included in CC17. Conclusions: The emergence and spread of new clones of VRE, especially in the hospital environment in this country, could become particularly problematic.

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“…Presence of a transposase (IS204/IS1001/IS1096/IS1165) between vanY and vanX was partially resolved. This vanA cluster constitutes a subtype of Tn1546-like elements which often bear IS elements like ISEf1, IS1678, or IS1216 V, ISEfa5, IS19-like between vanX and vanY (Werner et al, 2008;Jung et al, 2007;Camargo et al, 2005;Lopez et al, 2010). ermB, which encodes resistance to macrolides, lincosamides, and streptogramin B antibiotics was also identified on pLG1.…”

Section: Resistancementioning

confidence: 99%

A multiresistance megaplasmid pLG1 bearing a hylEfm genomic island in hospital Enterococcus faecium isolates

Gomez

Schaik

Freitas

et al. 2011

International Journal of Medical Microbiology

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Tn1546 structures and multilocus sequence typing of vanA-containing enterococci of animal, human and food origin

Cited by 35 publications

References 12 publications

Characterization of fecal vancomycin-resistant enterococci with acquired and intrinsic resistance mechanisms in wild animals, Spain

Characterization of fecal vancomycin-resistant enterococci with acquired and intrinsic resistance mechanisms in wild animals, Spain

Multidrug-resistant enterococci in the hospital environment: detection of novel vancomycin-resistant E. faecium clone ST910

A multiresistance megaplasmid pLG1 bearing a hylEfm genomic island in hospital Enterococcus faecium isolates

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