TMAO and other organic osmolytes in the muscles of amphipods (Crustacea) from shallow and deep water of Lake Baikal

Zerbst‐Boroffka, Irene; Kamaltynow, Ravil M.; Harjes, Stefan; Kinne-Saffran, Evamaria; Gross, Jürgen

doi:10.1016/j.cbpa.2005.07.008

Cited by 32 publications

(17 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on measurements of TMAO concentration in fresh and sea water species, 100 mM of TMAO is more than sufficient to provide protection against HHP 2 , 3 . Accordingly, most experiments in this study were performed using 100 mM TMAO.…”

Section: Resultsmentioning

confidence: 99%

“…In an attempt to explain the mechanisms of barotolerance, it has been reported that in tissues of deep-sea fish the concentration of trimethylamine N-oxide (TMAO) strongly correlates with depth at which these organisms live 2 , 3 . Hence, it appears that TMAO and similar organic osmolytes are key compounds providing barotolerance for deep-sea organisms.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The protective effect of osmoprotectant TMAO on bacterial mechanosensitive channels of small conductance MscS/MscK under high hydrostatic pressure

et al. 2012

View full text Add to dashboard Cite

Activity of the bacterial mechanosensitive channels of small conductance MscS/MscK of E. coli was investigated under high hydrostatic pressure (HHP) using the “flying-patch” patch-clamp technique. The channels were gated by negative pipette voltage and their open probability was measured at HHP of 0.1 to 80 MPa. The channel open probability decreased with increasing HHP. When the osmolyte methylamine N-oxide (TMAO) was applied to the cytoplasmic side of the inside-out excised membrane patches of E. coli giant spheroplasts the inhibitory effect of HHP on the channel activity was suppressed at pressures of up to 40 MPa. At 40 MPa and above the channel open probability decreased in a similar fashion with or without TMAO. Our study suggests that TMAO helps to counteract the effect of HHP up to 40 MPa on the MscS/MscK open state by “shielding” the cytoplasmic domain of the channels.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The protective effect of osmoprotectant TMAO on bacterial mechanosensitive channels of small conductance MscS/MscK under high hydrostatic pressure

et al. 2012

View full text Add to dashboard Cite

show abstract

“…By having a strong affinity for water, osmolytes can maintain cell volume by strongly binding to water in a cell. This is observed in deep sea fish as a method to combat high salt and high pressure environments . It has also been determined that osmolytes can stabilize proteins and are often used in protein formulations .…”

Section: Discussionmentioning

confidence: 99%

A generalized purification step for viral particles using mannitol flocculation

Heldt

Saksule

Joshi

et al. 2018

Biotechnology Progress

View full text Add to dashboard Cite

Vaccine manufacturing has conventionally been performed by the developed world using traditional unit operations like filtration and chromatography. There is currently a shift in the manufacturing of vaccines to the less developed world, requiring unit operations that reduce costs, increase recovery, and are amenable to continuous manufacturing. This work demonstrates that mannitol can be used as a flocculant for an enveloped and nonenveloped virus and can purify the virus from protein contaminants after microfiltration. The recovery of the virus ranges from 58 to 96% depending on virus, the filter pore size, and the starting concentration of the virus. Protein removal of 80% was achieved for the small nonenveloped virus using a 0.1 µm filter because proteins were not flocculated with the virus and flowed through the filter. It is hypothesized that mannitol dehydrates the viral surface by controlling the water structure surrounding the virus. Without the ability to become compact, as occurs with proteins, the virus aggregates in the presence of osmolytes and proteins do not. Osmolyte flocculation is a scalable process using high flux microfilters. It has been applied to both an enveloped and nonenveloped virus, making this process friendly to a variety of vaccine and gene therapy products. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:1027-1035, 2018.

show abstract

“…Several organic osmolytes, such as TMAO and betaine, which maintain osmolarity and protect cells from damage (Zerbst-Boroffka et al, 2005), were elevated in Kansui-treated rats. The formation of hydropic degeneration owing to the hypotonic conditions of extracellular fluid could correlate with the higher levels of TMAO and betaine.…”

Section: Liver and Kidney Injurymentioning

confidence: 99%

1H NMR-based metabonomics study of the urinary biochemical changes in Kansui treated rat

Tang

Ding

et al. 2012

Journal of Ethnopharmacology

View full text Add to dashboard Cite

TMAO and other organic osmolytes in the muscles of amphipods (Crustacea) from shallow and deep water of Lake Baikal

Cited by 32 publications

References 22 publications

The protective effect of osmoprotectant TMAO on bacterial mechanosensitive channels of small conductance MscS/MscK under high hydrostatic pressure

The protective effect of osmoprotectant TMAO on bacterial mechanosensitive channels of small conductance MscS/MscK under high hydrostatic pressure

A generalized purification step for viral particles using mannitol flocculation

1H NMR-based metabonomics study of the urinary biochemical changes in Kansui treated rat

Contact Info

Product

Resources

About