1H NMR-based metabonomics study of the urinary biochemical changes in Kansui treated rat

Tang, Bingwen; Ding, Jiajia; Wu, Fan; Chen, Lei; Yang, Yongxia; Song, Feifei

doi:10.1016/j.jep.2012.02.011

Cited by 35 publications

(16 citation statements)

References 42 publications

(43 reference statements)

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“…Assignments of endogenous metabolites involved in 1 H NMR spectra were based on the literature (Jung et al, 2011; Tang et al, 2012). The urinary NMR spectra were dominated by trimethylamine oxide (TMAO), taurine, betaine, glucose, creatine, creatinine, and tricarboxylic acid cycle (TCA cycle) intermediates, including α-ketoglutaric acid (α-KG), citrate, and succinate.…”

Section: Resultsmentioning

confidence: 99%

Metabolic Profiling Analysis of the Alleviation Effect of Treatment with Baicalin on Cinnabar Induced Toxicity in Rats Urine and Serum

Chen

et al. 2017

Front. Pharmacol.

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Objectives: Baicalin is the main bioactive flavonoid constituent isolated from Scutellaria baicalensis Georgi. The mechanisms of protection of liver remain unclear. In this study, 1H NMR-based metabonomics approach has been used to investigate the alleviation effect of Baicalin.Method: 1H NMR metabolomics analyses of urine and serum from rats, was performed to illuminate the alleviation effect of Baicalin on mineral medicine (cinnabar)-induced liver and kidney toxicity.Results: The metabolic profiles of groups receiving Baicalin at a dose of 80 mg/kg were remarkably different from cinnabar, and meanwhile, the level of endogenous metabolites returned to normal compared to group cinnabar. PLS-DA scores plots demonstrated that the variation tendency of control and Baicalein are apart from Cinnabar. The metabolic profiles of group Baicalein were similar to those of group control. Statistics results were confirmed by the histopathological examination and biochemical assay.Conclusion: Baicalin have the alleviation effect to the liver and kidney damage induced by cinnabar. The Baicalin could regulate endogenous metabolites associated with the energy metabolism, choline metabolism, amino acid metabolism, and gut flora.

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Section: Resultsmentioning

confidence: 99%

Metabolic Profiling Analysis of the Alleviation Effect of Treatment with Baicalin on Cinnabar Induced Toxicity in Rats Urine and Serum

Chen

et al. 2017

Front. Pharmacol.

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“…Published results indicated that metabonomics presented the potential capability to improve our understanding of TCM like QKL injection [19–22]. Recently, some advanced instruments and bio-information analysis techniques enable us to perform the simultaneous analysis of a large number of metabolites [23–26]. UPLC-Q-TOF/MS system can provide accurate mass of fragments, precursor ions, and neutral loss information, and it has a larger peak capacity and better resolution and sensitivity.…”

Section: Introductionmentioning

confidence: 99%

UPLC Q-TOF/MS-Based Metabolic Profiling of Urine Reveals the Novel Antipyretic Mechanisms of Qingkailing Injection in a Rat Model of Yeast-Induced Pyrexia

Gao

Guo

Peng

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Fever is one of the most common clinical symptoms of many diseases. Qingkailing (QKL) injection is widely used in China as a clinical emergency medicine due to its good antipyretic effects. It is a herbal formula which is composed by eight kinds of traditional Chinese medicines (TCM). As a kind of typical multiple constituents and multiple actions of TCM, it is very difficult to elaborate the antipyretic mechanism by conventional pharmacological method. Metabonomics technique provides beneficial tool for this challenge. In this study, an ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC Q-TOF/MS) metabonomics method was developed to explore the changing process of biochemical substances in rats of yeast-induced pyrexia. Partial least squares discriminate analysis (PLS-DA) was used to distinguish the normal control group, the pyrexia model group, and the pyrexia model group treated by QKL injection. The potential biomarkers related to pyrexia were confirmed and identified. MetPA was used to find the possible metabolic pathways. The results indicated that the antipyretic effect of QKL injection on yeast-induced pyrexia rats was performed by repairing the perturbed metabolism of amino acids.

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“…The acquisition parameters were essentially refer to those previously reported [28], [29]. The NMR spectrum was recorded using the water-presaturated standard one-dimensional Carr–Purcell–Meiboom–Gill (CPMG) pulse sequence, which can eliminate interference by macromolecules.…”

Section: Methodsmentioning

confidence: 99%

Combination of 1H NMR- and GC-MS-Based Metabonomics to Study on the Toxicity of Coptidis Rhizome in Rats

et al. 2014

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Background Coptidis Rhizome (CR), widely applied to treat with heat and toxicity, is one of the most commonly used traditional Chinese medicine (TCM), however, an extensive dosage can induce toxicity. Diarrhea is one of the most frequent side effects of CR treatment.Methodology/Principal FindingsIn this study, metabonomics was combined with the multivariate statistical analysis to discover the endogenous metabolites which related to the diarrheal induced by CR. The male Sprague–Dawley rats were dosed with 4.95 g CR/kg weight. Urine samples were collected at day −1 (before treatment), and days 14 and 21 for NMR analysis. Serum and tissues were collected at day 14 for GC-MS analysis and histopathological examination, respectively. The urine and serum metabolic profiles provided clearer distinction between CR-treated group and control group, which was confirmed by body weight change and diarrhea. Through multivariate statistical analysis, 12 marker metabolites from 1H NMR and 8 ones from GC-MS have been found. Among those metabolites, hippurate, acetate, alanine, glycine and glutamate are likely to break the balance of gut microbiota, whereas, lactate and 2-ketoisovalerate showed association with energy metabolism. Meanwhile, we observed that the CR-induced toxicity will recover when the treatment was stopped.Conclusions/significanceThese results suggest that the main reason for the CR-associated diarrhea might be disturbance in the normal gut microbiota. This metabonomics approach may provide an effective way to study the alteration of gut microbiota, which is expected to find broader application in other drug-induced gastrointestinal reaction assessment.

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1H NMR-based metabonomics study of the urinary biochemical changes in Kansui treated rat

Cited by 35 publications

References 42 publications

Metabolic Profiling Analysis of the Alleviation Effect of Treatment with Baicalin on Cinnabar Induced Toxicity in Rats Urine and Serum

Metabolic Profiling Analysis of the Alleviation Effect of Treatment with Baicalin on Cinnabar Induced Toxicity in Rats Urine and Serum

UPLC Q-TOF/MS-Based Metabolic Profiling of Urine Reveals the Novel Antipyretic Mechanisms of Qingkailing Injection in a Rat Model of Yeast-Induced Pyrexia

Combination of 1H NMR- and GC-MS-Based Metabonomics to Study on the Toxicity of Coptidis Rhizome in Rats

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