TLR2 Regulates Allergic Airway Inflammation and Autophagy Through PI3K/Akt Signaling Pathway

Jiang, Xuqin; Fang, Lei; Wu, Hui-Mei; Mei, Xiaodong; He, Fang; Ding, Peishan; Liu, Rong‐Yu

doi:10.1007/s10753-017-0581-x

Cited by 39 publications

(37 citation statements)

References 47 publications

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“…In this context, evidence has reported that IL‐5, IFN‐Y, and IL‐13 cytokines enhance autophagy (Ban et al, ; Jyothula & Eissa, ). In agreement to our results, evidence has reported that autophagy inhibition decreases inflammatory cytokines in the lung (Jiang et al, ; Liu et al, ). Moreover, autophagy plays a crucial role in the phosphorylation of NFκB (Criollo et al, ).…”

Section: Discussionsupporting

confidence: 94%

“…After that, we demonstrated that in the 3‐MA‐treated OVA group there was a reduction in goblet cells and mucus compared with the OVA group. In agreement to our result, evidence has shown that autophagy inhibition decreases goblet cells and mucus in the airway from the asthma model (Jiang et al, ; Liu et al, ). Poon et al () reported a relation between impairment in respiratory system mechanics in asthma and ATG polymorphism.…”

Section: Discussionsupporting

confidence: 94%

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Autophagy induces eosinophil extracellular traps formation and allergic airway inflammation in a murine asthma model

Silveira

Antunes

Kaiber

et al. 2019

Journal Cellular Physiology

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Studies have shown autophagy participation in the immunopathology of inflammatory diseases. However, autophagy role in asthma and in eosinophil extracellular traps (EETs) release is poorly understood. Here, we attempted to investigate the autophagy involvement in EETs release and in lung inflammation in an experimental asthma model. Mice were sensitized with ovalbumin (OVA), followed by OVA challenge.Before the challenge with OVA, mice were treated with an autophagy inhibitor, 3-methyladenine (3-MA). We showed that 3-MA treatment decreases the number of eosinophils, eosinophil peroxidase (EPO) activity, goblet cells hyperplasia, proinflammatory cytokines, and nuclear factor kappa B (NFκB) p65 immunocontent in the lung. Moreover, 3-MA was able to improve oxidative stress, mitochondrial energy metabolism, and Na + , K + -ATPase activity. We demonstrated that treatment with autophagy inhibitor 3-MA reduced EETs formation in the airway. On the basis of our results, 3-MA treatment can be an interesting alternative for reducing lung inflammation, oxidative stress, mitochondrial damage, and EETs formation in asthma. K E Y W O R D Sasthma, autophagy, eosinophil extracellular traps, eosinophils, inflammation

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 94%

Autophagy induces eosinophil extracellular traps formation and allergic airway inflammation in a murine asthma model

Silveira

Antunes

Kaiber

et al. 2019

Journal Cellular Physiology

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“…In addition, TLR2 signalling is involved in epithelial proliferation and apoptosis of terminally differentiated epithelial cells (Hormann et al, 2014). There are even indications that TLR2 signalling promotes mucin production (Jiang et al, 2017;Wu et al, 2007).…”

Section: Microbiota-independent Effects Of Dietary Fibrementioning

confidence: 99%

Microbiota‐dependent and ‐independent effects of dietary fibre on human health

Cai

Folkerts

et al. 2019

British J Pharmacology

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Dietary fibre, such as indigestible oligosaccharides and polysaccharides, occurs in many foods and has gained considerable importance related to its beneficial effects on host health and specific diseases. Dietary fibre is neither digested nor absorbed in the small intestine and modulates the composition of the gut microbiota. New evidence indicates that dietary fibre also interacts directly with the epithelium and immune cells throughout the gastrointestinal tract by microbiota‐independent effects. This review focuses on how dietary fibre improves human health and the reported health benefits that are connected to molecular pathways, in (a) a microbiota‐independent manner, via interaction with specific surface receptors on epithelial and immune cells regulating intestinal barrier and immune function, and (b) a microbiota‐dependent manner via maintaining intestinal homeostasis by promoting beneficial microbes, including Bifidobacteria and Lactobacilli, limiting the growth, adhesion, and cytotoxicity of pathogenic microbes, as well as stimulating fibre‐derived microbial short‐chain fatty acid production. Linked Articles This article is part of a themed section on The Pharmacology of Nutraceuticals. To view the other articles in this section visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v177.6/issuetoc

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“…The abnormal activation of PI3K-mTOR signaling pathway can also aggravate the airway in ammation in asthma [33][34][35]. It is also found that PI3K-mTOR signaling pathway can adjust autophagy and energy metabolism of cells to cope with the changes of internal and external environment [36,37].Baseline autophagy can maintain cell homeostasis, and involved in asthmatic airway in ammation. The overexpression of PI3K signaling pathway can lead to activation of mTOR signaling pathway [38,39].Autophagy is an intracellular waste treatment process.…”

Section: Discussionmentioning

confidence: 99%

“…Baseline autophagy can maintain cell homeostasis, and involved in asthmatic airway in ammation. The decrease of autophagy level can lead to the slow renewal of organelles and the deposition of discarded organelles, as well as up-regulation IL-1 β, IL-13 and TNF-α, suggesting that autophagy may be involved in the regulation of in ammation in patients with asthma [40].…”

Section: Discussionmentioning

confidence: 99%

Winyanghuayin Decoction and Xiaoqinglong Decoction Treatment Airway Inflammation of Cold Asthma Through PI3K-mTOR Signal Pathway

Chang

Zhang

Qing-xiang

et al. 2020

Preprint

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Abstract Background: Airway inflammation plays a pivotal role in cold asthma. Winyanghuayin decoction and Xiaoqinglong decoction can significantly improve airway inflammation. The aim of the present study was to explore the therapeutic effect and mechanism of Winyanghuayin decoction and Xiaoqinglong decoction on OVA-induced cold asthma in rat and compare the effects of Winyanghuayin decoction and Xiaoqinglong decoction for treatment of cold asthma ratsMethods: The cold asthma rat model was induced by OVA and ice water swim stress, treated with Winyanghuayin decoction and Xiaoqinglong decoction respectively. The pulmonary function of asthmatic rats was detected by MIR Spirolab; The pathological changes of lung tissue were observed by HE staining; The autophagy in the lung of rats were observed by transmission electron microscopy; The eosinophils, lymphocytes, macrophages and total cells in bronchoalveolar lavage fluid (BALF) were counted under light microscope. The expression of IL-10\IL-13\TNF-α and TGF-β1 in serum were measured by enzyme-linked immunosorbent assay (ELISA); The protein level of LC3-II\I, ATG, PI3K and mTOR expression in lung tissues were detected by Western blot; Real-time polymerase chain reaction (RT-PCR) detected the expression of autophagy gene ATG3\ATG5\ATG7 and ATG12.Results: Winyanghuayin decoction and Xiaoqinglong decoction can alleviate airway inflammation and improve airway hyper-responsiveness, significantly increased the level of autophagy in lung tissue and decreased the protein level of PI3K and mTOR in lung tissue of cold asthma rats. Winyanghuayin decoction have better regulation ability on autophagy and PI3K-mTOR signaling pathway.Conclusion: Winyanghuayin decoction and Xiaoqinglong decoction may alleviate the airway inflammation symptoms by enhancing autophagy in lung tissue dependent on the PI3K-mTOR signaling pathway. Winyanghuayin decoction was more effective than Xiaoqinglong decoction for treatment of cold asthma.

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TLR2 Regulates Allergic Airway Inflammation and Autophagy Through PI3K/Akt Signaling Pathway

Cited by 39 publications

References 47 publications

Autophagy induces eosinophil extracellular traps formation and allergic airway inflammation in a murine asthma model

Autophagy induces eosinophil extracellular traps formation and allergic airway inflammation in a murine asthma model

Microbiota‐dependent and ‐independent effects of dietary fibre on human health

Winyanghuayin Decoction and Xiaoqinglong Decoction Treatment Airway Inflammation of Cold Asthma Through PI3K-mTOR Signal Pathway

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