TLC and HPLC analysis of syringin in Syringa vulgaris

Куркин, В. А.; Grinenko, N. A.; Zapesochnaya, G. G.; Dubichev, A. G.; Vorontsov, E. D.

doi:10.1007/bf00629789

Cited by 7 publications

(6 citation statements)

References 2 publications

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“…Overall, syringin was much more abundant than coniferin. These results indicate that syringin is more abundant than coniferin and is mainly stored in bark, which is consistent with previous studies (Terazawa et al., ; Kurkin et al., , ). The free monolignols corresponding to the aglycon units of the monolignol glucosides, coniferyl alcohol, and sinapyl alcohol were present in trace amounts in lilac (); however, it was difficult to quantify them in tangential sections.…”

Section: Resultssupporting

confidence: 93%

“…Tissue classification of the bark region was performed by microscopy observations (). The abundant presence of syringin in the bark region agreed with the HPLC results (Figure ) and previous reports (Terazawa et al., ; Kurkin et al., , ). Therefore, we concluded that both the m / z 210 and 411 ions represent the syringin distribution in freeze‐fixed lilac stems.…”

Section: Resultssupporting

confidence: 92%

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Microscopic distribution of syringin in freeze‐fixed Syringa vulgaris stems

et al. 2019

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Monolignols are precursors of lignin, and their glucosides are often found in plants. Glucosylation creates water‐soluble and chemically stable monolignols by protecting the phenolic hydroxyl group. To discuss the role of sinapyl alcohol glucoside, syringin, in planta , the cellular distribution of syringin in the transverse and radial surfaces of quick‐frozen stems of Syringa vulgaris L. (lilac) was visualized by cryo‐time‐of‐flight secondary ion mass spectrometry and scanning electron microscopy (cryo‐TOF‐SIMS/SEM) analyses. The amount and rough distribution of syringin were confirmed by high‐performance liquid chromatography measurements using serial tangential sections of freeze‐fixed lilac stems. The syringin distribution was also discussed with reference to the tissue classification from microscopic observations. Syringin was mainly found in the phloem region. In the xylem region, syringin was evenly distributed irrespective of the cell type from the cambial zone to the early differentiating stage region and selectively distributed in vessels in the later differentiating stage region. After the lignification of wood fibers, syringin was found in rays and some vessels in the initial part of the annual rings. Previously, artificially administered isotope‐labeled syringin was shown to be assimilated into lignin in the differentiating xylem region. Based on this, our present data showing syringin storage in the differentiating xylem region and its variation depending on the lignification stage suggest that syringin works as a lignin precursor. Additionally, detection of syringin in vessels and rays indicates intercellular transportation of syringin in lilac stems.

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Section: Resultssupporting

confidence: 93%

Section: Resultssupporting

confidence: 92%

Microscopic distribution of syringin in freeze‐fixed Syringa vulgaris stems

et al. 2019

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“…eleutheroside B) and caffeic acid, respectively, using standards. The lilac bark contains syringin in high amounts (Kurkin et al ., ), but it is only present in trace amounts in flowers and fruits. Based on their molecular formula and known fragmentation pattern, hydroxytyrosol‐hexoside ( 4 ) and coniferin ( 6 ) could also be characterized in both extracts.…”

Section: Resultsmentioning

confidence: 99%

“…In spite of the potential pharmacological activities of lilac, only a few papers can be found describing the chemical composition of the plant. Available literature focuses mainly on the phenolic profile of the bark and leaf (Ahmad et al, 1995;Birkofer et al, 1968;Damtoft et al, 1995;Kurkin et al, 1989Kurkin et al, , 1992b. A limited number of papers can be found on the phytochemical composition of the other members of Syringa genus, such as S. oblata (Nenadis et al, 2007), S. afghanica (Takenaka et al, 2002), S. pubescens (Deng et al, 2010) and S. reticulate (Bi et al, 2011;Machida et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Characterization of antioxidant phenolics in Syringa vulgaris L. flowers and fruits by HPLC‐DAD‐ESI‐MS

Tóth

Barabás

Tóth

et al. 2015

Biomedical Chromatography

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In this study the polyphenolic composition of lilac flowers and fruits was determined for the first time. For the identification of compounds, accurate molecular masses and formulas, acquired by LC and ESI-TOF-MS and fragmentation pattern given by LC-ESI/MS/MS analyses, were used. Our chromatographic system in conjunction with tandem MS was found to be valuable in the rapid separation and determination of the multiple constituents in methanolic extracts of lilac flowers and fruits. Altogether 34 phenolics, comprising 18 secoiridoids, seven phenylpropanoids, four flavonoids and five low-molecular-weight phenols, were identified. As marker compounds two secoiridoids (oleuropein and nuzhenide), two phenylpropanoids (acteoside and echinacoside) and rutin were quantified by validated methods. As a result of quantitative analysis, it was confirmed that flowers contain significant amounts of phenylpropanoids (acteoside, 2.48%; echinacoside, 0.75%) and oleuropein (0.95%), while in fruits secoiridoid oleuropein (1.09%) and nuzhenide (0.42%) are the major secondary metabolites. The radical scavenging activities of the extracts and the constituents were investigated by DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS [2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)] assays. Both extracts show remarkable antioxidant activities. Our results clearly show that lilac flowers and fruits are inexpensive, readily available natural sources of phenolic compounds with pharmacological and cosmetic applications. Copyright © 2015 John Wiley & Sons, Ltd.

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“…Apparently the antiradical properties of both the aqueous and EtOH extracts of lilac are due to the presence in them of compounds in which the H-atom donors in the reaction with DPPH are non-phenolic compounds, e.g., phenylpropanoids, coumarins, iridoids, and derivatives of phenylethylalcohol and caffeic acid. Many of these, including the principal pharmacologically active component of lilac tinctures syringin (phenylpropanoid glycoside) exhibit ARA but are not polyphenols [14].…”

Section: Resultsmentioning

confidence: 99%

Composition and antiradical activity of lilac extracts

et al. 2011

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The composition and antioxidant properties of aqueous and EtOH extracts of the flowers and bark of various lilac species (Syringa vulgaris L., varieties Jeanne d'Arc and Lavoisier; S. josikaea Jacq. fil.; S. microphylla superba, and S. amurensis Rupr.) were investigated. It is found that the antiradical activity does not depend on the variety and species of lilac. Thus, any of the studied species can be used as pharmacological raw material. It is established that the antiradical properties of lilac extracts are determined to a considerable extent by compounds of nonphenolic nature.

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TLC and HPLC analysis of syringin in Syringa vulgaris

Cited by 7 publications

References 2 publications

Microscopic distribution of syringin in freeze‐fixed Syringa vulgaris stems

Microscopic distribution of syringin in freeze‐fixed Syringa vulgaris stems

Characterization of antioxidant phenolics in Syringa vulgaris L. flowers and fruits by HPLC‐DAD‐ESI‐MS

Composition and antiradical activity of lilac extracts

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