Titanium-Tethered Vancomycin Prevents Resistance to Rifampicin in Staphylococcus aureus in vitro

Rottman, Martin; Goldberg, Joel; Hacking, S. A.

doi:10.1371/journal.pone.0052883

Cited by 15 publications

(12 citation statements)

References 32 publications

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“…The details of surface energy calculation are given in Supplementary Information, the measurement and calculation results are listed in Table . With the surface energy data,

Δ G^{L W} true(d true)

Δ G^{A B} true(d true)

, and

Δ G^{E L} true(d true)

are calculated as follows:

Δ G^{L W} true(d true) = - \frac{A}{6} true[\frac{a}{d} + \frac{a}{d + 2 a} + \ln true(\frac{d}{d + 2 a} true) true]

where a is the bacterial radius which is about 0.5 μm for S. aureus ; and A is the Hamaker constant which can be calculated by

A = - 12 π d_{0}^{2} Δ G_{d_{0}}^{L W}

where d 0 denotes the distance of the closest approach between two surfaces, which is estimated as 1.57 Å; and

Δ G_{d_{0}}^{L W} = {true(\sqrt{γ_{B}^{L W}} - \sqrt{γ_{S}^{L W}} true)}^{2} - {true(\sqrt{γ_{B}^{L W}} - \sqrt{γ_{L B}^{L W}} true)}^{2} - {true(\sqrt{γ_{S}^{L W}} - \sqrt{γ_{L B}^{L W}} true)}^{2}

where subscripts B, S , and LB stand for bacterium ( S. aureus ), patterned silicon substrates and suspending liquid (LB medium), respectively. The values of

Δ G_{d_{0}}

…”

Section: Theoretical Analysismentioning

confidence: 99%

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Bacterial responses to periodic micropillar array

Leng

et al. 2014

J. Biomed. Mater. Res.

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For a basic understanding and potential biomedical applications of surface topographical effects on bacterial responses, this study focuses on not only the bacterial retention but also the bacterial growth, proliferation, and viability that are significant post-retentive behaviors playing critical roles in infections of medical implants. Specifically, periodic micropillar arrays (SiPA ) with nine different feature sizes were fabricated on silicon substrates with photolithography and dry etching methods. The SiPA was cultured with Staphylococcus aureus or Escherichia coli for different periods to investigate the bacterial retention, growth and proliferation behavior on a patterned surface. The experimental results show that a significant reduction of bacterial retention, growth, and proliferation can be achieved when the pillar size is reduced to the submicrometer level. However, micropillars have no obvious influence on the viability of the bacteria within 24 h. On the basis of the bacterial experiment results, it is inferred that the topographical effects may have resulted from bacterial confinement by micropillars, either limiting the attachment area for individual bacterium or trapping a bacterium between pillars. Furthermore, the extended Derjaguin-Landau-Verwey-Overbeek theoretical analysis indicates the effects might have come from the topographic induced surface property changes, mainly hydrophobicity, which is represented by the changes in the interaction free energy of Lifshitz-van der Waals among different periodic micropillar arrays. This study could help to deepen the understanding about the surface topographical effects on bacterial responses and may provide a guidance for the future medical implant surface design to decrease the infection risk by avoiding the surface topography which could attract more bacteria.

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“…The details of surface energy calculation are given in Supplementary Information, the measurement and calculation results are listed in Table . With the surface energy data,

Δ G^{L W} true(d true)

Δ G^{A B} true(d true)

, and

Δ G^{E L} true(d true)

are calculated as follows:

Δ G^{L W} true(d true) = - \frac{A}{6} true[\frac{a}{d} + \frac{a}{d + 2 a} + \ln true(\frac{d}{d + 2 a} true) true]

where a is the bacterial radius which is about 0.5 μm for S. aureus ; and A is the Hamaker constant which can be calculated by

A = - 12 π d_{0}^{2} Δ G_{d_{0}}^{L W}

where d 0 denotes the distance of the closest approach between two surfaces, which is estimated as 1.57 Å; and

Δ G_{d_{0}}^{L W} = {true(\sqrt{γ_{B}^{L W}} - \sqrt{γ_{S}^{L W}} true)}^{2} - {true(\sqrt{γ_{B}^{L W}} - \sqrt{γ_{L B}^{L W}} true)}^{2} - {true(\sqrt{γ_{S}^{L W}} - \sqrt{γ_{L B}^{L W}} true)}^{2}

where subscripts B, S , and LB stand for bacterium ( S. aureus ), patterned silicon substrates and suspending liquid (LB medium), respectively. The values of

Δ G_{d_{0}}

…”

Section: Theoretical Analysismentioning

confidence: 99%

“…where a is the bacterial radius which is about 0.5 lm for S. aureus 40,41 ; and A is the Hamaker constant which can be calculated by 39…”

Section: Theoretical Analysismentioning

confidence: 99%

Bacterial responses to periodic micropillar array

Leng

et al. 2014

J. Biomed. Mater. Res.

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“…When the implant itself damages or invades epithelial or mucosa barriers, it may serve as a reservoir for microorganisms thereby predisposing to infection. Once infection occurs, bacteria tend to aggregate in a hydrated polymeric matrix to form a bio-film on the implant which is difficult for the host defense and antimicrobial therapy to destroy [14], [15]. Such implant-related infections may lead to removal of the implant, revision surgery and even amputation, all of which are associated with extremely high medical costs.…”

Section: Introductionmentioning

confidence: 99%

Both Enhanced Biocompatibility and Antibacterial Activity in Ag-Decorated TiO2 Nanotubes

et al. 2013

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In this study, Ag is electron-beam evaporated to modify the topography of anodic TiO2 nanotubes of different diameters to obtain an implant with enhanced antibacterial activity and biocompatibility. We found that highly hydrophilic as-grown TiO2 nanotubes became poorly hydrophilic with Ag incorporation; however they could effectively recover their wettability to some extent under ultraviolet light irradiation. The results obtained from antibacterial tests suggested that the Ag-decorated TiO2 nanotubes could greatly inhibit the growth of Staphylococcus aureus. In vitro biocompatibility evaluation indicated that fibroblast cells exhibited an obvious diameter-dependent behavior on both as-grown and Ag-decorated TiO2 nanotubes. Most importantly, of all samples, the smallest diameter (25-nm-diameter) Ag-decorated nanotubes exhibited the most obvious biological activity in promoting adhesion and proliferation of human fibroblasts, and this activity could be attributed to the highly irregular topography on a nanometric scale of the Ag-decorated nanotube surface. These experimental results demonstrate that by properly controlling the structural parameters of Ag-decorated TiO2 nanotubes, an implant surface can be produced that enhances biocompatibility and simultaneously boosts antibacterial activity.

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“…[202][203] However, rapid release could also be observed for such systems, for instance within 50-150 min in the study by Popat et al 202 In order to obtain a more stable and controllable attachment of the biomolecule, which exhibits antibacterial activity for the longer term, the covalent immobilization of antibiotics on titanium-based surfaces has also been explored and has to a large extent focused on the attachment of the antibiotic vancomycin. [204][205][206][207] Furthermore, electrochemical processes have attracted attention for the stable immobilization of biomolecules on titanium surfaces. The incorporation of single stranded nucleic acids as an anchor within an anodically grown titanium oxide layer has been reported.…”

Section: Discussionmentioning

confidence: 99%

Effect of cathodic polarization on coating doxycycline on titanium surfaces

Geißler

Tiainen

2016

Materials Science and Engineering: C

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Cathodic polarization has been reported to enhance the ability of titanium based implant materials to interact with biomolecules by forming titanium hydride at the outermost surface layer. Although this hydride layer has recently been suggested to allow the immobilization of the broad spectrum antibiotic doxycycline on titanium surfaces, the involvement of hydride in binding the biomolecule onto titanium remains poorly understood. To gain better understanding of the influence this immobilization process has on titanium surfaces, mirror-polished commercially pure titanium surfaces were cathodically polarized in the presence of doxycycline and the modified surfaces were thoroughly characterized using atomic force microscopy, electron microscopy, secondary ion mass spectrometry, and angle-resolved X-ray spectroscopy. We demonstrated that no hydride was created during the polarization process. Doxycycline was found to be attached to an oxide layer that was modified during the electrochemical process. A bacterial assay using bioluminescent Staphylococcus epidermidis Xen43 showed the ability of the coating to reduce bacterial colonization and planktonic bacterial growth.

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Titanium-Tethered Vancomycin Prevents Resistance to Rifampicin in Staphylococcus aureus in vitro

Cited by 15 publications

References 32 publications

Bacterial responses to periodic micropillar array

Bacterial responses to periodic micropillar array

Both Enhanced Biocompatibility and Antibacterial Activity in Ag-Decorated TiO2 Nanotubes

Effect of cathodic polarization on coating doxycycline on titanium surfaces

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