Titanium dioxide anatase as matrix for matrix‐assisted laser desorption/ionization analysis of small molecules

Castro, A. L. de; Madeira, Paulo J. Amorim; Nunes, M. R.; Costa, F.M.; Florêncio, M. Helena

doi:10.1002/rcm.3795

Cited by 44 publications

(42 citation statements)

References 55 publications

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“…For TiO 2 powder developed marks, the best results were achieved from the sections subjected to re-enhancement using the initial powder. This was an unexpected observation as, although TiO 2 nanoparticles can be used as a MALDI matrix due to their UV absorbing properties 23 , previous results have shown that TiO 2 based fingermark enhancement powders cause a reduction in signal in comparison to when usi g α-CHCA alone 2 . Therefore, the authors speculate that the application of additional TiO 2 powder in these instances resulted in additional particle adherence to the fingermark ridges, subsequently enabling the recovery of more fingermark material during the secondary lift.…”

Section: Discussionmentioning

confidence: 92%

Development of operational protocols for the analysis of primary and secondary fingermark lifts by MALDI-MS imaging

2016

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Eight years of intensive research have demonstrated that Matrix-Assisted LaserDesorption/Ionisation Mass Spectrometry Profiling and Imaging (MALDI-MSI and MSP) are powerful tools to gather intelligence around a suspect lifestyle, directly from the identifying ridges of a latent fingermark. In the past three years, many efforts have been invested into translating laboratory methodologies to the field; this was undertaken by devising protocols either for (a) enabling initial fingermark visualisation, such as through the Dry-Wet method, recovery and subsequent MALDI MS based analysis, or for (b) rendering the MS methodologies compatible with the prior application of commonly employed fingermark enhancement techniques (FET). In the present work a major point of interest concerned the sample treatment of FET visualised-lifted fingermarks and the subsequent MS performance of primary tape lifted fingermarks ("primary lifts") versus secondary tape lifted fingermarks (recovery from the surface a second time following the initial primary lift). This was necessary since it may not always be possible to obtain primary lifts of marks visualised at crime scenes for remote MALDI-MSP and MSI. The work illustrated here has provided methodological insights into establishing how to best treat a few types of developed marks in preparation for MALDI-MSI when presented as both secondary and primary lifts; it was demonstrated, as expected, that primary lifts generally yield much higher quality chemical/physical information and are therefore crucial to maximise chances of suspect identification and of retrieval of chemical intelligence. When analysing secondary lifted marks that have been initially developed using aluminium or carbon powders, any of the trialled sample preparation methodologies can be employed except the Dry-Wet method. In the case of TiO 2 powder developed marks, the best ridge coverage was achieved by re-enhancing the mark using the initial powder and spray-coating with MALDI matrix. Primary lifts of fingermarks contaminated with an exogenous substance (used as a reference model) yielded the best ridge detail quality whilst for secondary lifts of natural marks pre-enhanced with aluminium powder, significantly greater intensity of the ion image was observed for the sections subjected to either no further enhancement or re-enhancement using aluminium powder.Keywords: Fingermarks; MALDI; Imaging; powders; lifts IntroductionWhile successful academic research is paramount for scientific progress, it is its translational feasibility that enables the impact of this research in the real world. With this view in mind, these authors are actively pursuing the development of protocols enabling MALDI-MS based technologies, with particular focus on the imaging modality, to be implemented into the current fingerprinting workflow.The pioneering development of MALDI-MS methodologies in fingermark analysis has a short but strong track record history of success, demonstrating the opportunity to retrieve additional devising an ...

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Section: Discussionmentioning

confidence: 92%

Development of operational protocols for the analysis of primary and secondary fingermark lifts by MALDI-MS imaging

2016

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“…Nanoparticles were suitable as MALDI matrices for small molecule imaging because of their uniform deposition over tissues (increasing lateral resolution) and the lack of background signals from matrix degradation. TiO 2 nanoparticles have been previously reported to be suitable for MALDI mass spectrometry analysis of low molecular weight compounds, with almost complete absence of background noise [40], and recently gold based nanoparticles have been reported to assist laser desorption/ionization, avoiding co-crystallization of the analyte and the matrix [41,42]. For the synthesis of AuNPs, we used a classical chemical reduction method that has been extensively used in the preparation of nanoparticles because this method is simple, cheap, and can be used to prepare large quantities of nanoparticles with an accurate shape and dimensions.…”

Section: Discussionmentioning

confidence: 99%

A Nanostructured Matrices Assessment to Study Drug Distribution in Solid Tumor Tissues by Mass Spectrometry Imaging

et al. 2017

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The imaging of drugs inside tissues is pivotal in oncology to assess whether a drug reaches all cells in an adequate enough concentration to eradicate the tumor. Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging (MALDI-MSI) is one of the most promising imaging techniques that enables the simultaneous visualization of multiple compounds inside tissues. The choice of a suitable matrix constitutes a critical aspect during the development of a MALDI-MSI protocol since the matrix ionization efficiency changes depending on the analyte structure and its physico-chemical properties. The objective of this study is the improvement of the MALDI-MSI technique in the field of pharmacology; developing specifically designed nanostructured surfaces that allow the imaging of different drugs with high sensitivity and reproducibility. Among several nanomaterials, we tested the behavior of gold and titanium nanoparticles, and halloysites and carbon nanotubes as possible matrices. All nanomaterials were firstly screened by co-spotting them with drugs on a MALDI plate, evaluating the drug signal intensity and the signal-to-noise ratio. The best performing matrices were tested on control tumor slices, and were spotted with drugs to check the ion suppression effect of the biological matrix. Finally; the best nanomaterials were employed in a preliminary drug distribution study inside tumors from treated mice.

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“…Due to strong absorption in the UV range and the ability to transfer energy to analytes rapidly, TiO 2 nanomaterials have been considered as one of the most promising matrices for LDI-TOF MS to analysis of small molecules. Castro et al [67] investigated the performance of TiO 2 with different types (anatase, rutile and their mixture) as matrix for LDI-TOF MS. Caffeine, PEG200, chloroaniline and quercetin could be ionized with TiO 2 anatase. TiO 2 pure forms, particularly anatase was proved to be a more useful matrix than their mixture because the materials with pure phase are more stabile under laser irradiation.…”

Section: Nanomaterial-assisted Ldi Methods Developmentmentioning

confidence: 99%

Nanomaterials as Assisted Matrix of Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for the Analysis of Small Molecules

Yang

et al. 2017

Nanomaterials

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Matrix-assisted laser desorption/ionization (MALDI), a soft ionization method, coupling with time-of-flight mass spectrometry (TOF MS) has become an indispensible tool for analyzing macromolecules, such as peptides, proteins, nucleic acids and polymers. However, the application of MALDI for the analysis of small molecules (<700 Da) has become the great challenge because of the interference from the conventional matrix in low mass region. To overcome this drawback, more attention has been paid to explore interference-free methods in the past decade. The technique of applying nanomaterials as matrix of laser desorption/ionization (LDI), also called nanomaterial-assisted laser desorption/ionization (nanomaterial-assisted LDI), has attracted considerable attention in the analysis of low-molecular weight compounds in TOF MS. This review mainly summarized the applications of different types of nanomaterials including carbon-based, metal-based and metal-organic frameworks as assisted matrices for LDI in the analysis of small biological molecules, environmental pollutants and other low-molecular weight compounds.

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Titanium dioxide anatase as matrix for matrix‐assisted laser desorption/ionization analysis of small molecules

Cited by 44 publications

References 55 publications

Development of operational protocols for the analysis of primary and secondary fingermark lifts by MALDI-MS imaging

Development of operational protocols for the analysis of primary and secondary fingermark lifts by MALDI-MS imaging

A Nanostructured Matrices Assessment to Study Drug Distribution in Solid Tumor Tissues by Mass Spectrometry Imaging

Nanomaterials as Assisted Matrix of Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for the Analysis of Small Molecules

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