Tissue‐specific markers in flow cytometry of urological cancers. III. Comparing chromosomal and flow cytometric dna analysis of bladder tumors

Abstract

Thirty-seven transitional-cell carcinomas (TCC) of the urinary bladder were analyzed by DNA flow cytometry (FCM). After labelling of the cell suspensions with antibodies to cytokeratin, the cytokeratin-positive cells and the non-epithelial cytokeratin-negative cells could be analyzed separately. After estimation of S- and G2M phase, 3/17 cases (18%) with a normal DNA index showed elevated proliferative levels, among cytokeratin-labelled suspensions only. Of these 17 cases, 14 showed chromosomal abnormalities. The remaining 20 cases were abnormal, irrespective of the technique used. Although immuno-labeling of tumor cells for cytokeratin in FCM increases the sensitivity of this method in detecting aneuploid tumors or tumors with high proliferation fractions, the discriminating power of chromosomal analysis of TCC is greater than FCM.

Complex karyotypes in flow cytometrically DNA-diploid squamous cell carcinomas of the head and neck

“…A 40-year-dd man with recumrrnt SCCHN of the tongue, clasfed as T3N1MO, with (A)-a-complex hypodpoid karyope: 40-44, XY dlc(1;11)(qlO;pII), der(3;19)(qlO;qlO), ins(4;?)(p14;? ), i(6)(pl0), i(6)(q10), ..de4(6q15), 1(8)(qlO), -11, der(13;14)(qlO;qlO), del (16) In the present study, DI values were consistently higher than CI values (Figure 2), a finding similar to those previously reported by others (Smeets et al, 1987;Remvikos et al, 1988b; , 1993). There are several possible cytogenetic explanations for this.…”

“…colorectal tumours, renal cell carcinoma and malignant mesothelioma, have usually shown good correspondence between DNA index and chromosome number (Petersen and Friedrich, 1986;Remvikos et al, 1988a;Ljungberg et al, 1991;Pyrhonen et al, 1992), although some discrepancies have also been noted (Smeets et al, 1987;Remvikos et al, 1988b;Wolman et al, 1988;El-Naggar and Pathak, 1992). Furthermore, it has been shown that a large proportion of flow cytometrically diploid tumours have karyotype abnormalities when analysed cytogenetically (Cabanillas et al, 1986;Smeets et al, 1987;Breitkreutz et al, 1993;Laquerriere et al, 1993;Mandahl et al, 1993;Matsuyama et al, 1994).…”

Flow cytometric analysis of DNA content and keratins by using CK7, CK8, CK18, CK19, and KL1 monoclonal antibodies in benign and malignant human breast tumors

“…In the present study, the use of double-labelling DNA and keratin FCM analysis allowed the DNA content to be analyzed in epithelial cells alone, as previously demonstrated in bladder, head, neck, and endometrium carcinoma (27,30). The debris background is gated out and the DNA histogram is obtained from the intact DNA+/CK+ cells.…”

“…Hence, the study of keratin expression in breast cancer could be of greater interest than as a simple epithelial marker. It has been shown in carcinomas of the bladder, head, neck, and endometrium that the use of monoclonal antibodies against keratins, together with DNA staining by flow cytometry (FCM), allows the analysis of the DNA content of the epithelial fraction (3,27,30). The simultaneous analysis of DNA and keratins by FCM might be of interest in the appreciation of the malignancy of human breast tumors and in the improvement of DNA content analysis.…”

A comparison of cytogenetic studies and flow cytometry in breast carcinomas

“…Flow cytometry (FCM) is commonly used for the analysis of cellular DNA content in malignant tumors. A few studies have combined results obtained on ploidy by FCM and cytogenetic (CG) analysis in different types of malignancies (2,4,12,17,18,22,29) including breast cancer (11,19).…”