A comparison of cytogenetic studies and flow cytometry in breast carcinomas

We present up to 45 years of cancer incidence data by occupational category for the Nordic populations. The study covers the 15 million people aged 30Á64 years in the 1960, 1970, 1980/1981 and/or 1990 censuses in Denmark, Finland, Iceland, Norway and Sweden, and the 2.8 million incident cancer cases diagnosed in these people in a follow-up until about 2005. The study was undertaken as a cohort study with linkage of individual records based on the personal identity codes used in all the Nordic countries.In the censuses, information on occupation for each person was provided through free text in self-administered questionnaires. The data were centrally coded and computerised in the statistical offices. For the present study, the original occupational codes were reclassified into 53 occupational categories and one group of economically inactive persons.All Nordic countries have a nation-wide registration of incident cancer cases during the entire study period. For the present study the incident cancer cases were classified into 49 primary diagnostic categories. Some categories have been further divided according to sub-site or morphological type. The observed number of cancer cases in each group of persons defined by country, sex, age, period and occupation was compared with the expected number calculated from the stratum specific person years and the incidence rates for the national population. The result was presented as a standardised incidence ratio, SIR, defined as the observed number of cases divided by the expected number. For all cancers combined (excluding non-melanoma skin cancer), the study showed a wide variation among men from an SIR of 0.79 (95% confidence interval 0.66Á0.95) in domestic assistants to 1.48 (1.43Á1.54) in waiters. The occupations with the highest SIRs also included workers producing beverage and tobacco, seamen and chimney sweeps. Among women, the SIRs varied from 0.58 (0.37Á0.87) in seafarers to 1.27 (1.19Á1.35) in tobacco workers. Low SIRs were found for farmers, gardeners and teachers.Our study was able to repeat most of the confirmed associations between occupations and cancers. It is known that almost all mesotheliomas are associated with asbestos exposure. Accordingly, plumbers, seamen and mechanics were the occupations with the highest risk in the present study. Mesothelioma was the cancer type showing the largest relative differences between the occupations. Outdoor workers such as fishermen, gardeners and farmers had the highest risk of lip cancer, while the lowest risk was found among indoor workers such as physicians and artistic workers.Studies of nasal cancer have shown increased risks associated with exposure to wood dust, both for those in furniture making and for those exposed exclusively to soft wood like the majority of Nordic woodworkers. We observed an SIR of 1.84 (1.66Á2.04) in male and 1.88 (0.90Á3.46) in female woodworkers. For nasal adenocarcinoma, the SIR in males was as high as 5.50 (4.60Á6.56).Male waiters and tobacco workers had the highest risk of ...

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“…Dietary habits among women varied less by occupation than among men but showed the same tendencies [183].…”

Section: General Lifestyle Factorsmentioning

confidence: 91%

Occupation and cancer – follow-up of 15 million people in five Nordic countries

et al. 2009

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“…Few studies have combined data from DNA cytometry and cytogenetic analysis on solid tumors, and little information is available on OSCC [18,40]. In 1999, EL Naggar reported a correlation between the DNA content evaluated by flow cytometry and the magnitude of the chromosomal aberration evaluated by fluorescence in situ hybridization (FISH) on 27 cases of OSCC [20].…”

Section: Discussionmentioning

confidence: 99%

Comparison of Cytogenetic and Static Cytometry Procedures in the Evaluation of Potentially Malignant Oral Lesions

Montebugnoli

Grelli

Cervellati

et al. 2008

Scholarly Research Exchange

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Gross genomic damage or specific chromosomal alterations have been revealed by different laboratory procedures in potentially malignant oral lesions, but two or more procedures have never been applied at the same time to the same cell population. In the present study we considered cell suspensions obtained from 34 oral lesions at risk of malignancy to see whether they might harbour genetic alterations and whether a correlation exists between the results obtained by two different methods of assessing DNA aberrations. Each suspension underwent DNA-content assessment by static cytometry, and cytogenetic G-banding analysis of short-term primary cultures. DNA content was determined in a minimum of 1000 cells on a Fairfield ploidy analyser and results expressed as percent of aneuploid cells in the S-phase; cytogenetic analysis was carried out according to standard procedures on in situ G-banding metaphases, and results expressed as percent of metaphases with chromosomal alterations. The results showed that the percentage of metaphases with chromosomal alterations was significantly correlated with the percentage of aneuploid cells in the S-phase. In conclusion, genetic alterations can be revealed in the same oral specimen either by procedures studying DNA content in fixed cells or by procedures investigating chromosomal alterations in cultured and proliferating cells.

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“…The last mentioned mechanisms result only in small changes of DNA-content. Cytogenetic examinations showed that this part of aneuploid clones in breast cancer, that has only a small deviation of DNA-content of stem cell population, could not be diagnozed by flow cytometry [31]. These subpopulations could not be assessed in a shared manner because of the limited resolution of G 0 G 1 -peak.…”

Section: Variation Coefficient Of G 0 G 1 -Peak Of Tumor Cellsmentioning

confidence: 99%

Evaluation of Prognostic Factors Following Flow‐Cytometric DNA Analysis after Cytokeratin Labelling: I. Breast Cancer

Wimberger

Hillemanns

Kapsner

et al. 2002

Analytical Cellular Pathology

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In gynecologic oncology valid prognostic factors are necessary to estimate the course of disease and to define biologically similar subgroups for analysis of therapeutic efficacy. The presented study is a prospective study concerning prognostic significance of DNA ploidy and S‐phase fraction in breast cancer following enrichment of tumor cells by cytokeratin labelling. Epithelial cells were labeled by FITC‐conjugated cytokeratin antibody (CK 5, 6, 8, and CK 17) prior to flow cytometric cell cycle analysis in 327 fresh specimens of primary breast cancer. Univariate analysis in breast cancer detected the prognostic significance of DNA‐ploidy, S‐phase fraction and CV (coefficient of variation) of G0G1‐peak of tumor cells for clinical outcome, especially for nodal‐negative patients. Multivariate analysis could not confirm prognostic evidence of DNA‐ploidy and S‐phase fraction. In conclusion, in breast cancer no clinical significance for determination of DNA‐parameters was found.

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A comparison of cytogenetic studies and flow cytometry in breast carcinomas

Cited by 11 publications

References 26 publications

Occupation and cancer – follow-up of 15 million people in five Nordic countries

Occupation and cancer – follow-up of 15 million people in five Nordic countries

Comparison of Cytogenetic and Static Cytometry Procedures in the Evaluation of Potentially Malignant Oral Lesions

Evaluation of Prognostic Factors Following Flow‐Cytometric DNA Analysis after Cytokeratin Labelling: I. Breast Cancer

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