Tissue-Specific Cytochrome Oxidase Activity in Largemouth Bass: The Metabolic Costs of Feeding and Growth

Goolish, Edward M.; Adelman, Ira R.

doi:10.1086/physzool.60.4.30157907

Cited by 64 publications

(31 citation statements)

References 42 publications

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“…In 6-monthold largemouth bass, Micropterus salmoides, the levels of CCO in muscle were positively correlated with growth rate (Goolish and Adelman, 1987). The "sh in the present study are also small "ngerlings, undergoing rapid increases in growth.…”

Section: Discussionsupporting

confidence: 56%

“…No signi"cant di!erence was detected in white muscle. It is possible that aerobic capacity was close to maximal in muscle, since young fast-growing "sh have a high metabolism (Goolish and Adelman, 1987) and, therefore, exposure to the di!erent treatments did not signi"cantly a!ect activity. This suggests that the gills and liver are more sensitive organs for detecting change in aerobic and anaerobic capacity, following exposure to crude oil and dispersed crude oil.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Metabolic Responses of Fish Following Exposure to Two Different Oil Spill Remediation Techniques

Cohen

Nugegoda

Gagnon

2001

Ecotoxicology and Environmental Safety

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Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Metabolic Responses of Fish Following Exposure to Two Different Oil Spill Remediation Techniques

Cohen

Nugegoda

Gagnon

2001

Ecotoxicology and Environmental Safety

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“…The lower RNA:DNA ratio in the head than in muscle could indicate that the head had lower growth rates than muscle tissues. The lower growth rate in the head has been related to a lower protein synthesis, or to lower cytochrome oxidase activity (Goolish and Adelman, 1987;Houlihan and Laurent, 1987). However, the similar RNA concentration in the head and in muscle observed in the present study does not point to a lower protein synthesis.…”

Section: Discussionsupporting

confidence: 53%

Tissue effect on RNA:DNA ratios of marine fish larvae

Olivar¹,

Diaz²,

Chı́charo³

2009

Sci. Mar.

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SUMMARY:In some routine studies of larval condition based on RNA:DNA ratios, heads and/or guts are removed for further age and feeding analysis. Also, during capture larvae are often found with their eyes missing. In this work we analysed tissues effects (muscle, head, eye, gut and the whole larvae) on RNA:DNA ratios from different species (Sardina pilchardus, Engraulis encrasicolus, Atherina presbyter and Paralichthys orbignyanus) in different developmental stages and from different locations. For all species and development phases, it was shown that RNA:DNA ratios in the head were significantly lower than in muscle or gut. The comparison of RNA:DNA ratios of head with or without eyes did not show significant differences. In a comparison of results from muscle from two tail sections, RNA:DNA ratios were higher for the anterior section in Sardina pilchardus and Engraulis encrasicolus larvae. Caution is needed when RNA:DNA ratios are compared with other studies employing different dissection protocols. For the species studied we propose conversion factors to be applied to RNA:DNA ratio data when different body sections are analysed. We discuss the most appropriate tissues for assessing nutritional condition in fish larvae, based on trends in concentrations of nucleic acids and ratios during starvation experiments.Keywords: RNA:DNA, tissues, starvation, condition, Sardina pilchardus, Engraulis encrasicolus, Atherina presbyter, Paralichthys orbignyanus. RESUMEN: Efecto del tipo de tejido sobre los cocientes ARN:ADN en larvas de peces marinos. -En estudios de condición nutricional larvaria basados en la relación ARN:ADN, las cabezas y/o tubos digestivos suelen diseccionarse para posteriores estudios de edad o alimentación. Además, durante la captura algunas larvas pierden los ojos. En este trabajo analizamos el efecto de diferentes tejidos (músculo, cabeza, ojos y tubo digestivo) sobre la relación ARN:ADN en Sardina pilchardus, Engraulis encrasicolus, Atherina presbyter y Paralichthys orbignyanus, en diferentes estadios de desarrollo y en diversas regiones. En todas las especies la relación ARN:ADN en la cabeza fue significativamente menor que para el músculo o el tubo digestivo. La comparación de la relación ARN:ADN de la cabeza con o sin ojos no mostró diferencias significativas. Al comparar dos secciones de músculo de la cola en Sardina pilchardus y Engraulis encrasicolus se observó mayor ARN:ADN en la parte anterior que en la posterior. Es necesaria precaución cuando se comparan ARN:ADN entre estudios que emplean diferentes protocolos de disección. Proponemos factores de conversión para ser aplicados a las relaciones ARN:ADN cuando se analizan diferentes secciones del cuerpo. Se discute cuales son los tejidos más apropiados para determinar la condición nutricional de larvas de peces, basados en las concentraciones de ácidos nucleicos y sus cocientes obtenidos en experimentos de inanición.

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“…In fish, muscle metabolic organization can also vary seasonally, independently of the effects of temperature (Kleckner and Sidell 1985;Vezina and Guderley 1991), possibly reflecting an impact of reproductive activities or photoperiod on enzyme activities. Changes in nutritional status and growth rate can also modify the metabolic organization of fish muscle (Sullivan and Somero 1983;Goolish and Adelman 1987;Kiessling et al 1990). Notably, Goolish and Adelman (1987) have shown a positive correlation between growth rate and the activity of cytochrome C oxidase (CCO) in muscle of largemouth bass (Micropterus salmoides).…”

Section: Introductionmentioning

confidence: 99%

“…Changes in nutritional status and growth rate can also modify the metabolic organization of fish muscle (Sullivan and Somero 1983;Goolish and Adelman 1987;Kiessling et al 1990). Notably, Goolish and Adelman (1987) have shown a positive correlation between growth rate and the activity of cytochrome C oxidase (CCO) in muscle of largemouth bass (Micropterus salmoides). Their intriguing interpretation of these results is that the aerobic capacity of muscle increases to meet the energetic requirements of protein synthesis.…”

Section: Introductionmentioning

confidence: 99%

Does the aerobic capacity of fish muscle change with growth rates?

Pelletier

Guderley

Dutil³

1993

Fish Physiol Biochem

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To ascertain whether growth rate modifies the oxidative capacity of fish white muscle, we examined the effects of individual growth rate on the activities of four mitochondrial enzymes in white muscle of the fast growing Atlantic cod,Gadus morhua. Growth rates were individually monitored in cod held at three acclimation temperatures during experiments repeated in four seasons. The size dependence of citrate synthase (CS), cytochrome C oxidase (CCO) and β-hydroxyacyl CoA dehydrogenase (HOAD) activities was established using wild cod ranging from 115 to 17,350 g. Given their negative allometry, CS and CCO activities in the experimental cod were corrected to those expected for a 1.2 kg animal. HOAD activities did not change with size. The specific activities of CCO and CS were positively correlated with growth rate. However, for both enzymes, season explained more of the variability than growth rate or temperature. Season was the only factor to significantly affect the activity of HOAD, while temperature and season interacted to determine glutamate dehydrogenase activity. CS activity was positively correlated with the initial condition of the cod, which differed among the seasons. The other enzymes did not show this relationship. The independent changes of these enzymes suggest that mitochondria undergo qualitative modifications with changes in growth rate, season and size. Although growth rate and the activities of CCO and CS are positively correlated, the activity of the mitochondrial enzymes is more affected by size, physical condition and season.

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Tissue-Specific Cytochrome Oxidase Activity in Largemouth Bass: The Metabolic Costs of Feeding and Growth

Cited by 64 publications

References 42 publications

Metabolic Responses of Fish Following Exposure to Two Different Oil Spill Remediation Techniques

Metabolic Responses of Fish Following Exposure to Two Different Oil Spill Remediation Techniques

Tissue effect on RNA:DNA ratios of marine fish larvae

Does the aerobic capacity of fish muscle change with growth rates?

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