Tissue Section Donovan Bodies – Identification through Slow-Giemsa (Overnight) Technique

Abstract

Slow-Giemsa (overnight) tissue section staining technique is described. It was found to be 100% successful in different variants of donovanosis. This method, though time-consuming, is much superior to often used tissue smears, and should be employed for its ultimate confirmation.

Phylogenetic evidence for reclassification of Calymmatobacterium granulomatis as Klebsiella granulomatis comb. nov.

“…One punch biopsy was fixed and examined for Donovan bodies by the slow Giemsa technique (Sehgal & Jain, 1987) whilst DNA was extracted from the second punch biopsy (seven samples) (Mullenbach et al, 1989) or swab (one sample) (Beige et al, 1995 t Sequences novel to this study.…”

A Case of Donovanosis in Japan

“…The paucity of reports of this disease in our country may be attributed to the widespread use of antibiotics, lack of knowledge about this bacterium as the causative organism of genital ulcers, difficulty in detecting the organism, and/or comparatively low racial susceptibility to donovanosis (3). The diagnosis of donovanosis can be established by examining smears or histologic sections by means of Giemsa staining, since Donovan bodies are very difficult to detect by haematoxylin-eosin staining (8). The disease is believed to be principally transmitted through sexual contact (9), although another view favours the hypothesis of intestinal colonization of the causative organisms.…”

Characterization of Circulating Lymphocytes by Monoclonal Antibodies in Donovanosis

“…The diagnosis of donovanosis was made in 65 patients on the basis of characteristic clinical features (1,6), and by demonstration of intracytoplasmic Donovan bodies in the Giemsa stained tissue smears (6). It was substantiated by specific histopathology (7) and slow (overnight) Giemsa stained tissue sections (8). All the patients were subjected to thorough physical examination to exclude any accompanying disease.…”