Tissue responses of calcium phosphate cement: a study in dogs

Yuan, Huipin; Li, Yubao; Bruijn, Joost D. de; Groot, K. de; Zhang, Xingdong

doi:10.1016/s0142-9612(00)00016-8

Cited by 276 publications

(199 citation statements)

References 30 publications

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“…The choice of rat femoral bone tissue as the defect site in the present study was based on technical rationale, such as easy handling, compatible bone structure, and functional resistance 11 . Both test and control defects were made in the rat left femur to maintain as much as possible the same variables for all groups.…”

Section: Analysis Of Optical Density Within Groupsmentioning

confidence: 99%

Evaluation of optical density of bone defects filled with calcium phosphate cement and bioactive glass in rats

et al. 2011

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Purpose: To evaluate new bone formation, by the analysis of optical density, in rat femoral defects filled with calcium phosphate cement (CPC) and bioactive glass (BG). Methods: Twenty-one rats were divided into three groups, Group I (CPC), Group II (BG), and Group III (control), and assessed after 7, 15, and 30 days. Three bone cavities were made in the left femur and filled with CPC, BG, and no material (control). Digital images were obtained and the results were subjected to statistical analysis of variance (ANOVA), complemented by the Friedman and Kruskal-Wallis nonparametric tests, with a significance level of 5%. Results: Regarding optical density, Group I showed statistical values significantly higher than Group III and also higher, although not statistically significant, than Group II, in all observation periods. When Groups II and III were compared, Group II showed higher optical density values, without statistically significant differences, in all periods. Conclusion: The biomaterials analyzed showed higher optical density in relation to the control group in all observation periods, calcium phosphate cement being the best option in the repair of bone defects, but without statistically significant differences in relation to bioactive glass.

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Section: Analysis Of Optical Density Within Groupsmentioning

confidence: 99%

Evaluation of optical density of bone defects filled with calcium phosphate cement and bioactive glass in rats

et al. 2011

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“…Ripamonti et al (1989) reported bone induction in a composite allogeneic bone/alloplastic implant; Zhang et al (1991), Osborn (1991), and Ripamonti (1991a) discovered bone formation at ectopic sites after implantation of porous calcium phosphate ceramic materials in dogs and baboons. Later, osteoinductive bioceramics began to be reported by different labs in pigs, sheep, rabbits, and other large animal models all over the world (Damien and Parsons, 1991;Toth et al, 1993;Li et al, 1994;Yuan et al, 2000;2001a;Nihouannen et al, 2005;Ye et al, 2007;Fellah et al, 2008). Since then, generous attention was paid to these kinds of biomaterials, such as synthetic hydroxyapatite ceramics (HA), porous biphasic calcium phosphate ceramics (BCP), tricalcium phosphate ceramics (TCP), calcium pyrophosphate ceramics, and coralderived hydroxyapatite.…”

Section: Introductionmentioning

confidence: 99%

Osteoinduction by Ca-P biomaterials implanted into the muscles of mice

Yang

Cheng

et al. 2011

J. Zhejiang Univ. Sci. B

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Abstract:The osteoinduction of porous biphasic calcium phosphate ceramics (BCP) has been widely reported and documented, but little research has been performed on rodent animals, e.g., mice. In this study, we report osteoinduction in a mouse model. Thirty mice were divided into two groups. BCP materials (Sample A) and control ceramics (Sample B) were implanted into the leg muscle, respectively. Five mice in each group were killed at 15, 30, and 45 d after surgery. Sample A and Sample B were harvested and used for hematoxylin and eosin (HE) staining, immunohistochemistry (IHC) staining, and Alizarin Red S staining to check bone formation in the biomaterials. Histological analysis showed that no bone tissue was formed 15 d after implantation (0/5) in either of the two groups. Newly-formed bone tissues were observed in Sample A at 30 d (5/5) and 45 d (5/5) after implantation; the average amounts of newly-formed bone tissues were approximately 5.2% and 8.6%, respectively. However, we did not see any bone tissue in Sample B until 45 d after implantation. Bone-related molecular makers such as bone morphogenesis protein-2 (BMP-2), collagen type I, and osteopontin were detected by IHC staining in Sample A 30 d after implantation. In addition, the newly-formed bone was also confirmed by Alizarin Red S staining. Because this is the report of osteoinduction in the rodent animal on which all the biotechnologies were available, our results may contribute to further mechanism research.

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“…Osteoinduction has been shown in soft tissues of various animal models by a number of calcium-phosphate (CaP) containing bulk ceramics such as hydroxyapatite (HA), 2-9 a-tricalcium phosphate (a-TCP), b-TCP-, BCP, a-pyrophosphate, and b-pyrophosphate. 2,3,6,10 -18 Furthermore, ectopic bone formation has been observed by octacalcium phosphate (OCP)-coated porous biomaterials, 12,13,19 CaP cements, 11,20 and glass ceramics. 21 Osteoinduction has also been reported by porous alumina ceramic 22 and titanium.…”

Section: Introductionmentioning

confidence: 99%

Relevance of Osteoinductive Biomaterials in Critical‐Sized Orthotopic Defect

Habibović

Yuan

Doel

et al. 2006

Journal Orthopaedic Research

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163

168

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Several publications have shown the phenomenon of osteoinduction by biomaterials to be real. However, whether the ability of a biomaterial to initiate bone formation in ectopic implantation sites improves the performance of such osteoinductive biomaterial in clinically relevant orthotopic sites remains unclear. No studies have been published in which osteoinductive potential of a biomaterial is directly related to its performance orthotopically. In this study, we compared osteoinductive and nonosteoinductive biphasic calcium-phosphate (BCP) ceramics ectopically and in a clinically relevant critical-sized orthotopic defect in goats. The two materials, BCP1150 and BCP1300, had similar chemical compositions, crystallinities, and macrostructures, but their microstructures differed significantly. BCP1150, sintered at a lower temperature, had a large amount of micropores, small average crystal size, and hence a high specific surface area. In contrast, BCP1300, with few micropores, had a significantly lower specific surface area as compared to BCP1150. Twelve-week intramuscular implantation in goats (n ¼ 10) showed that bone was induced in all BCP1150 implants, while no signs of bone formation were found in any of the BCP1300 implants. After 12 weeks of implantation in a bilateral critical-sized iliac wing defect in the same goats, BCP1150 showed significantly more bone than BCP1300. In addition, the analysis of fluorochrome markers, which were administered to the animals 4, 6, and 8 weeks after implantation to follow the bone growth dynamics, showed an earlier start of bone formation in BCP1150 as compared to BCP1300. Significantly better performance of an osteoinductive ceramic in a criticalsized orthotopic defect in a large animal model in comparison to a nonosteoinductive ceramic suggests osteoinduction to be clinically relevant. Further improvement of material osteoinductive properties is thus a significant step forward in the search for alternatives for autologous bone graft.

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Tissue responses of calcium phosphate cement: a study in dogs

Cited by 276 publications

References 30 publications

Evaluation of optical density of bone defects filled with calcium phosphate cement and bioactive glass in rats

Evaluation of optical density of bone defects filled with calcium phosphate cement and bioactive glass in rats

Osteoinduction by Ca-P biomaterials implanted into the muscles of mice

Relevance of Osteoinductive Biomaterials in Critical‐Sized Orthotopic Defect

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