Tissue PSA from fine-needle biopsies of prostatic carcinoma as related to serum PSA, clinical stage, cytological grade, and DNA ploidy

Stege, Reinhard; Tribukait, Bernhard; Carlström, Kjell; Grande, Mirtha; Pousette, Åke

doi:10.1002/(sici)1097-0045(19990215)38:3<183::aid-pros2>3.0.co;2-w

Cited by 25 publications

(17 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This finding is consistent with prior observations showing a discrepancy between serum PSA levels and tissue PSA expression (24,25), and serves as a reminder that changes in serum PSA concentrations are not necessarily an accurate surrogate for tissue response. Because androgens influence prostatic vasculature (35)(36)(37), the decline in serum PSA may reflect changes in tissue vascularity and PSA leak (38).…”

Section: Discussionmentioning

confidence: 99%

Intraprostatic Androgens and Androgen-Regulated Gene Expression Persist after Testosterone Suppression: Therapeutic Implications for Castration-Resistant Prostate Cancer

et al. 2007

View full text Add to dashboard Cite

Androgen deprivation therapy (ADT) remains the primary treatment for advanced prostate cancer. The efficacy of ADT has not been rigorously evaluated by demonstrating suppression of prostatic androgen activity at the target tissue and molecular level. We determined the efficacy and consistency of medical castration in suppressing prostatic androgen levels and androgen-regulated gene expression. Androgen levels and androgen-regulated gene expression (by microarray profiling, quantitative reverse transcription-PCR, and immunohistochemistry) were measured in prostate samples from a clinical trial of short-term castration (1 month) using the gonadotropin-releasing hormone antagonist, Acyline, versus placebo in healthy men. To assess the effects of long-term ADT, gene expression measurements were evaluated at baseline and after 3, 6, and 9 months of neoadjuvant ADT in prostatectomy samples from men with localized prostate cancer. Medical castration reduced tissue androgens by 75% and reduced the expression of several androgen-regulated genes (NDRG1, FKBP5, and TMPRSS2). However, many androgen-responsive genes, including the androgen receptor (AR) and prostatespecific antigen (PSA), were not suppressed after short-term castration or after 9 months of neoadjuvant ADT. Significant heterogeneity in PSA and AR protein expression was observed in prostate cancer samples at each time point of ADT. Medical castration based on serum testosterone levels cannot be equated with androgen ablation in the prostate microenvironment. Standard androgen deprivation does not consistently suppress androgen-dependent gene expression. Suboptimal suppression of tumoral androgen activity may lead to adaptive cellular changes allowing prostate cancer cell survival in a low androgen environment. Optimal clinical efficacy will require testing of novel approaches targeting complete suppression of systemic and intracrine contributions to the prostatic androgen microenvironment. [Cancer Res 2007;67(10):5033-41]

show abstract

Section: Discussionmentioning

confidence: 99%

Intraprostatic Androgens and Androgen-Regulated Gene Expression Persist after Testosterone Suppression: Therapeutic Implications for Castration-Resistant Prostate Cancer

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Similar to prior studies, there is no relationship between tissue PSA/HK3 mRNA and serum PSA measurements (10 -13). Although Stege et al (11) showed an inverse relationship between tissue PSA and serum PSA, they used fine-needle aspiration techniques to assess the tumor PSA expression.…”

Section: Discussionmentioning

confidence: 99%

Higher Expression of the Androgen-Regulated Gene PSA/HK3 mRNA in Prostate Cancer Tissues Predicts Biochemical Recurrence-Free Survival

Sterbis

Gao

Furusato

et al. 2008

Clinical Cancer Research

View full text Add to dashboard Cite

Purpose: Alterations of the androgen receptor (AR)-mediated signaling through numerous mechanisms are increasingly recognized in prostate cancer (CaP) progression.We hypothesized that the assessment of well-defined AR transcriptional targets (e.g., PSA/HK3 mRNA) in CaP tissues will provide in vivo readout of AR dysfunctions. Moreover, quantitative expression features of PSA/HK3 mRNA inprostate tumor cells may serve as a prognosticindicator of disease progression. Experimental Design: Paired benign and malignant epithelial cells (242 specimens) were obtained from laser capture microdissection of frozen OCT-embedded tissue sections prepared from radical prostatectomy specimens of121patients. Quantitative expression of PSA/HK3 mRNA in the matched malignant and benign cells was analyzed by real-time reverse transcription-PCR. Results: CaP cells express significantly lower PSA/HK3 mRNA levels than matched benign cells (P = 0.0133). Moreover, low PSA/HK3 mRNA expression in malignant cells was associated with increased risk of biochemical recurrence (P = 0.0217), as well as with time to recurrence (P = 0.0371), in patients with intermediate preoperative serum prostate-specific antigen levels (2-10 ng/mL). The expression of androgen-dependent genes in clinical samples correlates with each other in patients with higher expression of PSA/HK3 mRNA but not in patients with lower expression of PSA/HK3 mRNA reflecting AR pathway dysfunction. Conclusions: Our study has unraveled a novel prognostic utility of quantitative measurements of PSA/HK3 mRNA reflecting AR transcriptional activity in CaP cells, which is independent of serum prostate-specific antigen. It also has potential in stratifying subsets of patients exhibiting progressive disease associated with dampened AR transcriptional functions who may be targeted by tailored therapeutic strategies.

show abstract

“…This has also been described in biochemical investigations (11)(12)(13). In a prospective study of 179 hormonally treated patients with prostate carcinoma, tissue PSA was the dominating prognostic factor.…”

Section: Discussionmentioning

confidence: 58%

“…With the decrease in tissue PSA, S-PSA increased significantly (11). A low level of tissue PSA was associated with poor prognosis in hormonally treated patients (12,13). By using molecular biological techniques, the same cDNA sequence of tissue PSA was found in benign and malignant tissue (14).…”

mentioning

confidence: 95%

See 1 more Smart Citation

Double‐fluorescence image microscopy for quantitation of prostate‐specific antigen in histologic sections of the prostate

Wang¹,

Tribukait²

2002

Cytometry

Self Cite

View full text Add to dashboard Cite

Tissue PSA from fine-needle biopsies of prostatic carcinoma as related to serum PSA, clinical stage, cytological grade, and DNA ploidy

Cited by 25 publications

References 16 publications

Intraprostatic Androgens and Androgen-Regulated Gene Expression Persist after Testosterone Suppression: Therapeutic Implications for Castration-Resistant Prostate Cancer

Intraprostatic Androgens and Androgen-Regulated Gene Expression Persist after Testosterone Suppression: Therapeutic Implications for Castration-Resistant Prostate Cancer

Higher Expression of the Androgen-Regulated Gene PSA/HK3 mRNA in Prostate Cancer Tissues Predicts Biochemical Recurrence-Free Survival

Double‐fluorescence image microscopy for quantitation of prostate‐specific antigen in histologic sections of the prostate

Contact Info

Product

Resources

About