Tissue-nonspecific alkaline phosphatase promotes the osteogenic differentiation of osteoprogenitor cells

Nakamura, Takashi; Nakamura-Takahashi, Aki; Kasahara, Masataka; Yamaguchi, Akira; Azuma, Toshifumi

doi:10.1016/j.bbrc.2020.01.136

Cited by 48 publications

(29 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results imply different modes of TNAP functional reduction and might point to different biochemical consequences. TNAP/Tnap's promoting role in hydroxyapatite crystallization is mediated by diminishing the amount of prevalent mineralization inhibitor PP i in the extracellular space during in vitro osteogenic differentiation 69 . While axonal outgrowth is initiated by preventing the activation of the purinergic receptor of P 2 X 7 7,10 .…”

Section: Discussionmentioning

confidence: 99%

Investigation of alpl expression and Tnap-activity in zebrafish implies conserved functions during skeletal and neuronal development

Ohlebusch

Borst²,

Frankenbach³

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Different tissues express different alkaline phosphatase (AP) isoforms and HPP patients' phenotypes can greatly differ in the responsiveness between tissues 1. Remarkably, comparative studies have already shown that the predominant TNAP transcript variant in bones is the same as in brain tissues 6 , which implies a common mode of TNAP function in both tissues. TNAP's most important biochemical function in bones and teeth is providing the basis for mineralization processes. The enzyme enables hydroxyapatite crystallization in bone via catalyzing the dephosphorylation of mineralization inhibitors such as inorganic pyrophosphate (PP i) and phosphorylated osteopontin 7. In the nervous system, two predominant mechanisms, the availability of vitamin B6 and alterations in purinergic signaling 8,9 , significantly influence the outcome of the disease. In case of decreased TNAP-activity, pyridoxal-5-phosphate (PLP), which is the transportable form of vitamin B6, accumulates within the serum and cannot be redistributed into the brain without dephosphorylation via TNAP. PLP is an essential enzymatic co-factor within central neurotransmitter synthesis pathways in the brain and the lack of PLP results in neurological impairment due to limited biochemical conversion 8. Additionally, TNAP enzyme supports the development and maintenance of synapse functionality and is involved in the outgrowth and myelination of neurites 10-12. Localization of TNAP within primates' brains has been described as distinct patterns within layer 4 and 5 of the cortex 13,14 and has been detected in the retina across a number of different vertebrate species 15. In mice, TNAP localization was detected predominantly in endothelial cells, primordial germ cells, pioneer growth cones, and neural precursors 16. Akp2 (the murine version of the human ALPL gene) knockout mice display reduced serum levels of alkaline phosphatase, elevated substrate levels, impaired bone mineralization, and frequently die from epileptic seizures 17,18. Due to the early lethal phenotype in mice and the lack of supplementary in vivo studies within other vertebrates, our knowledge about TNAP's interconnected functions within bone and neuronal tissues is still scarce. Due to a number of biological properties, the zebrafish (Danio rerio) has become an important vertebrate model organism for bone 19,20 and brain research 21. Along with other advantages, including low cost and easy housing of the animals, the possibility to investigate transgenic reporter lines by non-invasive in vivo monitoring and perform large scale screenings, zebrafish offer a wide repertoire of different approaches for investigation of bone and brain development 22-24. Furthermore, basic molecular pathways for vertebrate development, like bone development, are evolutionarily conserved and share common properties with other species 25,26. Consequently, a rising number of in vivo models for musculoskeletal diseases have been established in recent years 27-29 and even the molecular background of complex diseases, such...

show abstract

Section: Discussionmentioning

confidence: 99%

Investigation of alpl expression and Tnap-activity in zebrafish implies conserved functions during skeletal and neuronal development

Ohlebusch

Borst²,

Frankenbach³

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Recent studies have shown that although hArg had no effect on TNAP levels in TNAP-overexpressing mice, it did offer some protection from myocardial remodeling through mechanisms unrelated to TNAP inhibition [ 92 ]. Although levamisole has been used in several studies to help identify the role that TNAP plays in health and disease [ 33 , 75 , 99 ], it is a reversible inhibitor, so cells treated with this drug rapidly regain TNAP activity [ 100 ]. In order to identify small molecule inhibitors to target specific AP isoforms, Millan and colleagues employed high-throughput screening approaches and identified several small molecule aryl sulfonamides as potent and selective TNAP inhibitors [ 99 ].…”

Section: Models and Tools To Study Tnapmentioning

confidence: 99%

Tissue-Nonspecific Alkaline Phosphatase in Central Nervous System Health and Disease: A Focus on Brain Microvascular Endothelial Cells

Nwafor

Brichacek

Ali

et al. 2021

IJMS

View full text Add to dashboard Cite

Tissue-nonspecific alkaline phosphatase (TNAP) is an ectoenzyme bound to the plasma membranes of numerous cells via a glycosylphosphatidylinositol (GPI) moiety. TNAP’s function is well-recognized from earlier studies establishing its important role in bone mineralization. TNAP is also highly expressed in cerebral microvessels; however, its function in brain cerebral microvessels is poorly understood. In recent years, few studies have begun to delineate a role for TNAP in brain microvascular endothelial cells (BMECs)—a key component of cerebral microvessels. This review summarizes important information on the role of BMEC TNAP, and its implication in health and disease. Furthermore, we discuss current models and tools that may assist researchers in elucidating the function of TNAP in BMECs.

show abstract

“…ALP is a marker of early differentiation of osteoblasts and has an important effect on osteoblast mineralization [56], therefore ALP expression in osteoblasts directly reflects the differentiation state of osteoblasts. As shown in Fig.…”

Section: Osteoblast Growth Behaviors and Function Expressionmentioning

confidence: 99%

Incorporation of heparin/BMP2 complex on GOCS-modified magnesium alloy to synergistically improve corrosion resistance, anticoagulation, and osteogenesis

Lin

Yang

Zhao

et al. 2021

J Mater Sci: Mater Med

View full text Add to dashboard Cite

The in vivo fast degradation and poor biocompatibility are two major challenges of the magnesium alloys in the field of artificial bone materials. In this study, graphene oxide (GO) was first functionalized by chitosan (GOCS) and then immobilized on the magnesium alloy surface, finally the complex of heparin and bone morphogenetic protein 2 was incorporated on the modified surface to synergistically improve the corrosion resistance, anticoagulation, and osteogenesis. Apart from an excellent hydrophilicity after the surface modification, a sustained heparin and BMP2 release over 14 days was achieved. The corrosion resistance of the modified magnesium alloy was significantly better than that of the control according to the results of electrochemical tests. Moreover, the corrosion rate was also significantly reduced in contrast to the control. The modified magnesium alloy not only had excellent anticoagulation, but also can significantly promote osteoblast adhesion and proliferation, upregulate the expression of alkaline phosphatase and osteocalcin, and enhance mineralization. Therefore, the method of the present study can be used to simultaneously improve the corrosion resistance and biocompatibility of the magnesium alloys targeted for the orthopedic applications.

show abstract

Tissue-nonspecific alkaline phosphatase promotes the osteogenic differentiation of osteoprogenitor cells

Cited by 48 publications

References 27 publications

Investigation of alpl expression and Tnap-activity in zebrafish implies conserved functions during skeletal and neuronal development

Investigation of alpl expression and Tnap-activity in zebrafish implies conserved functions during skeletal and neuronal development

Tissue-Nonspecific Alkaline Phosphatase in Central Nervous System Health and Disease: A Focus on Brain Microvascular Endothelial Cells

Incorporation of heparin/BMP2 complex on GOCS-modified magnesium alloy to synergistically improve corrosion resistance, anticoagulation, and osteogenesis

Contact Info

Product

Resources

About