Tissue mimetic hyaluronan bioink containing collagen fibers with controlled orientation modulating cell migration and alignment

Schwab, Albrecht; Hélary, Christophe; Richards, Rebecca; Alini, Mauro; Eglin, David; D’Este, Matteo

doi:10.1016/j.mtbio.2020.100058

Cited by 60 publications

(73 citation statements)

References 74 publications

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“…These components were incorporated in gelatin nanoparticles and proven to support the proliferation of HepG2 cells [66]. A bioink containing a tyramine-derivative HA and aligned collagen type I fibers, has been used for MSC differentiation towards chondrocytes [67].…”

Section: Surfacesmentioning

confidence: 99%

Extracellular Matrix Mimics Using Hyaluronan-Based Biomaterials

Amorim

Reis

et al. 2021

Trends in Biotechnology

100

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Hyaluronan (HA) is a critical element of the extracellular matrix (ECM). The regulated synthesis and degradation of HA modulates the ECM chemical and physical properties that, in turn, influence cellular behavior. HA triggers signaling pathways associated with the adhesion, proliferation, migration, and differentiation of cells, mediated by its interaction with specific cellular receptors or by tuning the mechanical properties of the ECM. This review summarizes the recent advances on strategies used to mimic the HA present in the ECM to study healthy or pathological cellular behavior. This includes the development of HA-based 2D and 3D in vitro tissue models for the seeding and encapsulation of cells, respectively, and HA particles as carriers for the targeted delivery of therapeutic agents. Highlights The extracellular matrix is composed of hyaluronan of different molecular weights that play different roles in cellular proliferation, migration, and differentiation.

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Section: Surfacesmentioning

confidence: 99%

Extracellular Matrix Mimics Using Hyaluronan-Based Biomaterials

Amorim

Reis

et al. 2021

Trends in Biotechnology

100

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“…Hydrogels are natural polymers widely used for their excellent biocompatibility and ECM mimetism [ 101 ]. Hydrogels are usually polysaccharides (e.g., alginate, HA) [ 102 , 103 ] or protein-based (collagen, fibrin) [ 89 , 103 ].…”

Section: Bioextrusion Processes For Cartilage Tissue Engineeringmentioning

confidence: 99%

“…MSCs are usually embedded in a hydrogel to reproduce the hyaline-like cartilaginous matrix, due to their excellent hydration properties, such as alginate [ 162 ] GelMA [ 163 ] or dECM-based bioinks [ 106 ]. An important aspect of tissue mimetism is the fiber organization within the different layers; the bioextrusion process can print layers with different alignments, making it possible to reproduce the collagen fibers’ natural organization within the cartilaginous ECM [ 103 ]. The addition of compounds that lead to differentiation was investigated at length.…”

Section: Bioextrusion Processes For Cartilage Tissue Engineeringmentioning

confidence: 99%

“…To reduce shear stress during extrusion, nozzle diameter and geometry are vital factors [ 117 ]. Other printing parameters, such as cross-linking (UV light, ionic, or enzymatic gelation), also need to be tuned to permit maximum cell survival [ 60 , 103 , 119 , 165 , 166 ]. To assess the long-term effects of the overall printing process, many researchers evaluated viability at different times postprinting [ 162 , 165 , 166 ].…”

Section: Bioextrusion Processes For Cartilage Tissue Engineeringmentioning

confidence: 99%

“…In contrast, other biomaterials, such as gelatin, silk, or collagen, can improve cell nesting and survival [ 154 , 155 ]. Other studies used cell aggregates or spheroids to seed into the bioink instead of single cells [ 103 , 175 ]. A comparison of embedded spheroids to isolated cells in bioextruded substitutes demonstrated a better survival of cells inside spheroids [ 175 ].…”

Section: Bioextrusion Processes For Cartilage Tissue Engineeringmentioning

confidence: 99%

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Stem Cells and Extrusion 3D Printing for Hyaline Cartilage Engineering

Messaoudi

Henrionnet

Bourge

et al. 2020

Cells

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Hyaline cartilage is deficient in self-healing properties. The early treatment of focal cartilage lesions is a public health challenge to prevent long-term degradation and the occurrence of osteoarthritis. Cartilage tissue engineering represents a promising alternative to the current insufficient surgical solutions. 3D printing is a thriving technology and offers new possibilities for personalized regenerative medicine. Extrusion-based processes permit the deposition of cell-seeded bioinks, in a layer-by-layer manner, allowing mimicry of the native zonal organization of hyaline cartilage. Mesenchymal stem cells (MSCs) are a promising cell source for cartilage tissue engineering. Originally isolated from bone marrow, they can now be derived from many different cell sources (e.g., synovium, dental pulp, Wharton’s jelly). Their proliferation and differentiation potential are well characterized, and they possess good chondrogenic potential, making them appropriate candidates for cartilage reconstruction. This review summarizes the different sources, origins, and densities of MSCs used in extrusion-based bioprinting (EBB) processes, as alternatives to chondrocytes. The different bioink constituents and their advantages for producing substitutes mimicking healthy hyaline cartilage is also discussed.

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Advancing Engineered Heart Muscle Tissue Complexity with Hydrogel Composites

Dickerson

2022

Advanced Biology

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A heart attack results in the permanent loss of heart muscle and can lead to heart disease, which kills more than 7 million people worldwide each year. To date, outside of heart transplantation, current clinical treatments cannot regenerate lost heart muscle or restore full function to the damaged heart. There is a critical need to create engineered heart tissues with structural complexity and functional capacity needed to replace damaged heart muscle. The inextricable link between structure and function suggests that hydrogel composites hold tremendous promise as a biomaterial‐guided strategy to advance heart muscle tissue engineering. Such composites provide biophysical cues and functionality as a provisional extracellular matrix that hydrogels cannot on their own. This review describes the latest advances in the characterization of these biomaterial systems and using them for heart muscle tissue engineering. The review integrates results across the field to provide new insights on critical features within hydrogel composites and perspectives on the next steps to harnessing these promising biomaterials to faithfully reproduce the complex structure and function of native heart muscle.

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Tissue mimetic hyaluronan bioink containing collagen fibers with controlled orientation modulating cell migration and alignment

Cited by 60 publications

References 74 publications

Extracellular Matrix Mimics Using Hyaluronan-Based Biomaterials

Extracellular Matrix Mimics Using Hyaluronan-Based Biomaterials

Stem Cells and Extrusion 3D Printing for Hyaline Cartilage Engineering

Advancing Engineered Heart Muscle Tissue Complexity with Hydrogel Composites

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