Tissue lipid metabolism and hepatic metabolomic profiling in response to supplementation of fermented cottonseed meal in the diets of broiler chickens

Abstract

This study investigated the effects of fermented cottonseed meal (FCSM) on lipid metabolites, lipid metabolism-related gene expression in liver tissues and abdominal adipose tissues, and hepatic metabolomic profiling in broiler chickens. One hundred and eighty 21-d-old broiler chickens were randomly divided into three diet groups with six replicates of 10 birds in each group. The three diets consisted of a control diet supplemented with unfermented cottonseed meal, an experimental diet of cottonseed meal fermented by Candida tropicalis, and a second experimental diet of cottonseed meal fermented by C. tropicalis plus Saccharomyces cerevisae. The results showed that FCSM intake significantly decreased the levels of abdominal fat and hepatic triglycerides (P<0.05 for both). Dietary FCSM supplementation down-regulated the mRNA expression of fatty acid synthase and acetyl CoA carboxylase in liver tissues and the lipoprotein lipase expression in abdominal fat tissues (P<0.05 for both). FCSM intake resulted in significant metabolic changes of multiple pathways in the liver involving the tricarboxylic acid cycle, synthesis of fatty acids, and the metabolism of glycerolipid and amino acids. These findings indicated that FCSM regulated lipid metabolism by increasing or decreasing the expression of the lipid-related gene and by altering multiple endogenous metabolites. Lipid metabolism regulation is a complex process, this discovery provided new essential information about the effects of FCSM diets in broiler chickens and demonstrated the great potential of nutrimetabolomics in researching complex nutrients added to animal diets.

Effects of microbial fermented sesame meal and enzyme supplementation on the intestinal morphology, microbiota, pH, tibia bone and blood parameters of broiler chicks

“…In agreement with results of present study, Ashayerizadeh et al (2018) showed that serum cholesterol, triglycerides and VLDL-C contents of broiler chicken fed fermented rapeseed meal diets were lower than other treatments. Similar results that showed a decrease in triglyceride level using fermented products (fermented cottonseed Meal) in the broiler chicken diets have been reported by Nie et al (2015). The reduction mechanisms of cholesterol and triglycerides that were previously reported have a close relation with the population of the lactic acid bacteria.…”

Effects of yeast cultures with different fermentation times on the growth performance, caecal microbial community and metabolite profile of broilers

“…Akkermansia can thicken the mucosal layer of the intestinal tract, improve metabolism, alter the way the body treats fat and stimulate the growth of one or more bacterial species, which can greatly improve the growth performance (Lozupone et al, 2012;Nie et al, 2015). Akkermansia can thicken the mucosal layer of the intestinal tract, improve metabolism, alter the way the body treats fat and stimulate the growth of one or more bacterial species, which can greatly improve the growth performance (Lozupone et al, 2012;Nie et al, 2015).…”

“…We observed a marked increase in the abundance of Akkermansia within the phylum Verrucomicrobia in broilers fed YC24. Akkermansia can thicken the mucosal layer of the intestinal tract, improve metabolism, alter the way the body treats fat and stimulate the growth of one or more bacterial species, which can greatly improve the growth performance (Lozupone et al, 2012;Nie et al, 2015). Although these findings indicate that Akkermansia are beneficial bacteria, others have reported that Akkermansia muciniphila can induce weight gain and obesity in mice and humans (Everard et al, 2011(Everard et al, , 2013Santacruz et al, 2010).…”

Modulation of broilers’ caecal microflora and metabolites in response to a potential probiotic Bacillus amyloliquefaciens

“…The metabolite detection followed the methods of Nie et al. (). Briefly, the caecal content was homogenised with L‐2‐Chlorophe and blended with the chilled extraction liquid (methanol and chloroform).…”