Modulation of broilers’ caecal microflora and metabolites in response to a potential probiotic <i>Bacillus amyloliquefaciens</i>

Cao, Guangtian; Zhan, Xiuan; Zhang, L. L.; Zeng, Xiangfang; Chen, A. G.; Yang, Caimei

doi:10.1111/jpn.12856

Cited by 34 publications

(40 citation statements)

References 26 publications

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“…and reduced Escherichia coli levels in the intestinal contents of DFMsupplemented birds, compared with controls. Increased growth performance, nutrient utilization, and intestinal villus/crypt morphometry, as well as altered composition of gut microbiota, also were observed in chickens orally administered with Bacillus DFMs, compared with controls (32)(33)(34)(35).…”

Section: Discussionmentioning

confidence: 93%

“…Given the reported effects of DFMs on modulating the chicken gut microbiome (10)(11)(12), and the ability of intestinal bacteria to synthesize vitamins and nutrients beneficial to the host (43), it is somewhat surprising that few reports have examined the ability of DFMs to alter the avian metabolome (35,44). Cao et al (35) reported that dietary supplementation of chickens with B. amyloliquefaciens altered the levels of gut metabolites related to amino acid and glyceride metabolism. More specifically, increased levels of 4-aminobutyric acid, gentiobiose, quinic acid, 3,7,12-trihydroxycoprostane, N-ethylglycine, glycine, N-acetyl-D-galactosamine, and 5hydroxyindole-3-acetic acid, and reduced the levels of diglycerol and N-acetyl-β-D-mannosamine, were seen in the DFMsupplemented chickens, compared with unsupplemented controls.…”

Section: Discussionmentioning

confidence: 99%

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Dietary Supplementation With Bacillus subtilis Direct-Fed Microbials Alters Chicken Intestinal Metabolite Levels

Park

Zimmerman²,

Smith³

et al. 2020

Front. Vet. Sci.

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Direct-fed microbials (DFMs) are dietary supplements containing live microorganisms which confer a performance and health benefit to the host, but the mechanisms are unclear. Here, a metabolomics approach was used to identify changes in intestinal metabolite levels in chickens fed an unsupplemented diet or a diet supplemented with B. subtilis strain 1781 or strain 747. Body weight gains of chickens fed the B. subtilis-supplemented diets were increased up to 5.6% in the B. subtilis 1781 group and 7.6% in the B. subtilis 747 group compared with chickens fed the unsupplemented diet. Compared with unsupplemented controls, the levels of 83 metabolites were altered (p < 0.05) (25 increased, 58 decreased) in chickens given the B. subtilis 1781-supplemented diet, while 50 were altered (p < 0.05) (12 increased, 38 decreased) with the B. subtilis 747-supplemented diet. Twenty-two metabolites were altered (p < 0.05) (18 increased, 4 decreased) in the B. subtilis 1781 vs. B. subtilis 747 groups. A random forest analysis of the B. subtilis 1781 vs. control groups gave a predictive accuracy of 87.5%, while that of the B. subtilis 747 vs. control groups was 62.5%. A random forest analysis of the B. subtilis 1781 vs. B. subtilis 747 groups gave a predictive accuracy of 75.0%. Changes in the levels of these intestinal biochemicals provided a distinctive biochemical signature unique to each B. subtilis-supplemented group, and were characterized by alterations in the levels of dipeptides (alanylleucine, glutaminylleucine, phenylalanylalanine, valylglutamine), nucleosides (N1-methyladenosine, N6-methyladenosine, guanine, 2-deoxyguanosine), fatty acids (sebacate, valerylglycine, linoleoylcholine), and carbohydrates (fructose). These results provide the foundation for future studies to identify biochemicals that might be used to improve poultry growth performance in the absence of antibiotic growth promoters.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Dietary Supplementation With Bacillus subtilis Direct-Fed Microbials Alters Chicken Intestinal Metabolite Levels

Park

Zimmerman²,

Smith³

et al. 2020

Front. Vet. Sci.

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“…Beyond inherent basic nutrition, oral probiotics also exert many beneficial effects on health. Several species of Bacillus can function as antibiotic alternatives and growth promoters as they improve the digestibility and absorption of nutrients in the intestines of pigs and birds (Hong et al, 2005; Cao et al, 2018). Especially, emerging evidence has suggested that Bacillus amyloliquefaciens (BA) is beneficial for the amelioration of diarrhea and inflammation (Li et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Bacillus amyloliquefaciens Ameliorates Dextran Sulfate Sodium-Induced Colitis by Improving Gut Microbial Dysbiosis in Mice Model

Cao

Wang

et al. 2019

Front. Microbiol.

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Several Bacillus strains exert beneficial effects on the maintenance of intestinal homeostasis and host health. However, whether Bacillus amyloliquefaciens (BA) can improve gut microbial dysbiosis and ameliorate colitis is unknown. Therefore, we conducted the present study to investigate the effects of BA administration on intestinal morphology, inflammatory response, and colonic microbial composition in a mouse model of dextran sulfate sodium (DSS)-induced colitis. Results showed that BA administration significantly ameliorated body weight loss, decreased disease activity index, and improved colonic tissue morphology in DSS-treated mice. In addition, levels of immunoglobulins, as well as pro-inflammatory cytokines, were decreased after BA administration. Importantly, colonic microbiota profiling indicated a significant (p < 0.05) difference in beta-diversity between BA-administrated and DSS-treated mice, according to weighted principal coordinate analysis (PCoA) results. The relative abundance of the Firmicutes genus was increased, whereas that of Bacteroidetes was decreased by BA administration. Furthermore, phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) analysis showed that the most significantly changed pathways between the four groups of mice were carbohydrate, lipid, and amino acid metabolism. In conclusion, our results showed that BA administration has beneficial effects on DSS-induced colitis, suggesting that this strategy might be useful for the treatment of dysbiosis during ulcerative colitis. Further, the changes in metabolism, especially amino acid metabolism, might contribute to the beneficial effects of BA on the amelioration of DSS-induced colitis.

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“…In addition, DFMs are also found to be suitable for chickens to reduce pathogen colonization and invasion in the intestinal tract to prevent several enteric infections such as Salmonella Enteritidis (14), E. coli (15), and Clostridium perfringens (16). Among the DFMs, Bacillus-based products have become more popular for potential use in broiler diets as alternatives to antibiotic growth promoters to improve both performance and health (17). Due to their spore-forming ability, these bacteria can withstand harsh environmental conditions including during feed processing and pelleting, as well as survive and germinate under conditions of the gastrointestinal tract (18,19).…”

Section: Introductionmentioning

confidence: 99%

“…Bacillus amyloliquefaciens is a spore-forming probiotic bacterium that produces a variety of extracellular enzymes including α-amylases, proteases, and phytase which could improve digestion and absorption of certain nutrients. Studies with Bacillus amyloliquefaciens have reported improved growth performance and villus morphology (20), modified cecal microbiota and metabolites (17), and increased serum IgG and IgA concentrations of healthy broilers (21). Li et al (22) suggested that dietary supplementation of Bacillus amyloliquefaciens downregulated mRNA abundance of TLR-4, INFγ, and IL-1β, and improved intestinal barrier junction in LPS-challenged broilers.…”

Section: Introductionmentioning

confidence: 99%

Effects of Dietary Direct Fed Microbial Supplementation on Performance, Intestinal Morphology and Immune Response of Broiler Chickens Challenged With Coccidiosis

et al. 2019

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Poultry coccidiosis is a costly intestinal disease that leads to considerable tissue damage, inefficient nutrient absorption, increased mortality, and predisposition to secondary infections. This study evaluated the effects of a direct feed microbial (DFM) dietary additive on performance, intestinal morphology, and immune response of broilers during a mixed coccidiosis challenge. In total, 840 Cobb500 male broilers were randomly allocated to 3 treatments (7 replicates, 40 birds/pen) including negative control (NC) fed basal diet; positive control (PC) fed basal diet with coccidiosis challenge; and DFM supplemented diet, with coccidiosis challenge. At 15 days of age, all birds except for the NC treatment were orally gavaged with live oocysts of a commercial vaccine. On d 21 (6 days post challenge), 4 birds/pen were randomly selected and euthanized for scoring of coccidia-caused lesions in the duodenum, jejunum, and ceca. Body weight gain (BWG), feed intake (FI), and feed conversion ratio (FCR) were recorded on d 7, 14, 28, and 42. Jejunal and ileal tissue samples were taken for histomorphological assessment from 2 birds/pen on d 21. Ileal samples were also taken for mRNA expression analysis on d 14 and d 21. The DFM birds had significantly greater BWG than PC birds during d 0-21 (P < 0.05). No differences were observed among the treatment groups in terms of FI and FCR. Dietary DFM supplementation significantly reduced lesion scores in the duodenum and jejunum when compared with PC group (P < 0.05). The coccidia challenge significantly reduced (P < 0.05) ileal villus height when compared to the non-challenged group on d 21. Conversely, dietary DFM supplementation alleviated the negative effects of coccidiosis by increasing ileal villus area on d 21 (P < 0.05). The challenged birds had significantly greater expression of IFN-γ and IL-1β in the ileum on d 21. Based on these findings, dietary DFM supplementation may help restore broiler performance during the starter and early grower periods during coccidiosis, likely by maintaining gut integrity via improving intestinal morphology and also by reducing disease severity as manifested by lower lesion scores.

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Modulation of broilers’ caecal microflora and metabolites in response to a potential probiotic Bacillus amyloliquefaciens

Cited by 34 publications

References 26 publications

Dietary Supplementation With Bacillus subtilis Direct-Fed Microbials Alters Chicken Intestinal Metabolite Levels

Dietary Supplementation With Bacillus subtilis Direct-Fed Microbials Alters Chicken Intestinal Metabolite Levels

Bacillus amyloliquefaciens Ameliorates Dextran Sulfate Sodium-Induced Colitis by Improving Gut Microbial Dysbiosis in Mice Model

Effects of Dietary Direct Fed Microbial Supplementation on Performance, Intestinal Morphology and Immune Response of Broiler Chickens Challenged With Coccidiosis

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