Tissue expression of CD10 protein in colorectal carcinoma: correlation with the anatomopathological features of the tumor and with lymph node and liver metastases

Abstract

Tissue expression of CD10 protein in colorectal carcinoma: correlation with the anatomopathological features of the tumor and with lymph node and liver metastases. J Coloproctol, 2012;32(1): 34-9. AbstrACt: background:The reduced expression of CD10 may be related to unfavorable prognosis of patients with colorectal carcinoma. The authors analyzed the tissue immunostaining of CD10 protein in colorectal carcinoma and its relationship to clinicopathologic features. Method: In 130 patients submitted to colorectal carcinoma surgery, a tissue microarray block was obtained from the tumor and adjacent non-neoplastic mucosa and submitted to immunohistochemistry with monoclonal antibody CD10. The immunostaining was evaluated by semi-quantitative method, with stained cell count in percentage. The results were related to the location, anatomopathological features, presence of lymph node and hepatic metastases and TNM staging of the colorectal neoplasm. The statistical analysis was performed with the Mann-Whitney, Kruskal-Wallis and Fisher exact tests. results: The expression of CD10 marker was higher in colorectal tumor tissue than in adjacent non-neoplastic mucosa (p<0.0001) and was higher than in exophytic lesions (p=0.04). The expression of CD10 protein was not associated with other clinical and pathological aspects of colorectal neoplasm. Conclusions: The expression of CD10 protein was more intense in tumor tissue of colorectal carcinoma than in adjacent non-neoplastic mucosa and was related to the exophytic appearance of the tumor.Keywords: neoplasm antigens; colorectal neoplasms; prognosis; neoplasm metastasis; CD10 antigens. resuMO: Introdução: A expressão reduzida de CD10 pode estar relacionada com prognóstico desfavorável de doentes com carcinoma colorretal. Analisou-se a imunoexpressão tecidual da proteína CD10 no carcinoma colorretal e sua relação com os aspectos clinicopatológicos. Método: Em 130 doentes operados por carcinoma colorretal, um bloco de tissue microarray foi obtido do tecido neoplásico e da mucosa não neoplásica adjacente e submetido ao estudo imuno-histoquímico com anticorpo monoclonal CD10. Avaliou-se a imunoexpressão por método semiquantitativo, com contagem do percentual de células coradas. Os resultados foram relacionados com a localização, aspectos anatomopatológicos, presença de metástases linfonodais e hepáticas e estadiamento TNM da neoplasia. O estudo estatístico foi realizado com os testes de Mann-Whitney, Kruskal-Wallis e exato de Fisher. resultados: A expressão do marcador CD10 foi maior no tecido do carcinoma colorretal do que na mucosa não neoplásica adjacente (p<0,0001) e foi maior nas lesões exofíticas (p=0,04). A expressão da proteína CD10 não apresentou relação com os demais aspetos clínicos e patológicos da neoplasia colorretal. Conclusões: A expressão da proteína CD10 foi mais intensa no tecido neoplásico do carcinoma colorretal do que na mucosa não neoplásica adjacente e relacionou-se com o aspecto exofítico do tumor. Palavras

Neutral endopeptidase (NEP) is differentially involved in biological activities and cell signaling of colon cancer cell lines derived from various stages of tumor development

“…Few of these studies, however, implicate NEP, a cell membrane-bound peptidase, in colon cancer development and metastasis. Most reports evaluate only the relationship between the expression level of the peptidase and the stage/grade or metastasis development of CC in vivo [15–21]. There are some reports which implicate NEP in colon cancer metastasis to the liver but not in biological functions of primary and secondary tumors developing in other sites, e.g., lymph nodes [13, 14].…”

“…On the other hand, de Oliveira et al and Oshima et al did not observe any differences in NEP expression corresponding to histological differentiation [15, 19]. Lymphatic, vascular, and/or perineural invasion of colorectal carcinoma was associated with NEP expression according to Fujimoto et al and Yao et al, whereas de Oliveira et al and Fujita et al did not observe any significant relationships in this respect [15, 18, 20, 21]. Moreover, there are some controversies regarding the expression level of the peptidase and metastasis.…”

“…The studies are discrepant in some aspects. De Oliveira et al and Sato et al, for instance, have reported that the level of NEP expression was higher in CC tissue samples than in non-neoplastic mucosa adjacent to the tumor, whereas Ogawa et al did not find any expression of this marker in samples of normal tissue [15–17]. Sato et al and Fujimoto et al identified a higher expression level of NEP in well or/and moderately differentiated adenocarcinoma tissue specimens in comparison with poorly differentiated ones [16, 18].…”

“…Sato et al and Fujimoto et al identified a higher expression level of NEP in well or/and moderately differentiated adenocarcinoma tissue specimens in comparison with poorly differentiated ones [16, 18]. On the other hand, de Oliveira et al and Oshima et al did not observe any differences in NEP expression corresponding to histological differentiation [15, 19]. Lymphatic, vascular, and/or perineural invasion of colorectal carcinoma was associated with NEP expression according to Fujimoto et al and Yao et al, whereas de Oliveira et al and Fujita et al did not observe any significant relationships in this respect [15, 18, 20, 21].…”

“…Fujimoto et al and Yao et al have identified a strong expression of this marker in patients with a higher incidence of liver metastases [18, 21]. On the other hand, de Oliveira et al and Fujita et al did not find any such relationship [15, 20]. …”