Tissue engineering of articular cartilage: From bench to bed-side

Rahman, Rozlin Abdul; Radzi, Muhammad Aa'zamuddin Ahmad; Sukri, Norhamiza Mohamad; Nazir, Noorhidayah; Sha'ban, Munirah

doi:10.1007/s13770-014-9044-8

Cited by 16 publications

(22 citation statements)

References 74 publications

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“…However, a long-term expansion of cells often leads to dedifferentiation of the chondrocytes. Thus the maintenance of cartilage phenotype is another important consideration in cartilage tissue engineering [34][35][36]. Within hydrogels composed of some natural materials including alginate and agarose, the chondrocytes were shown to maintain in part their chondrogenic phenotype, suggesting the importance of 3D gel matrix environment for chondrocytes culture [37][38][39].…”

Section: Discussionmentioning

confidence: 99%

Effects of Type I Collagen Concentration in Hydrogel on the Growth and Phenotypic Expression of Rat Chondrocytes

Jin

Kim

2017

Tissue Eng Regen Med

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It is controversial whether type I collagen itself can maintain and improve chondrogenic phenotype of chondrocytes in a three-dimensional (3D) environment. In this study, we examined the effect of type I collagen concentration in hydrogel (0.5, 1, and 2 mg/ml) on the growth and phenotype expression of rat chondrocytes in vitro. All collagen hydrogels showed substantial contractions during culture, in a concentration-dependent manner, which was due to the cell proliferation. The cell viability was shown to be the highest in 2 mg/ml collagen gel. The mRNA expression of chondrogenic phenotypes, including SOX9, type II collagen, and aggrecan, was significantly up-regulated, particularly in 1 mg/ml collagen gel. Furthermore, the production of type II collagen and glycosaminoglycan (GAG) content was also enhanced. The results suggest that type I collagen hydrogel is not detrimental to, but may be useful for, the chondrocyte culture for cartilage tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Effects of Type I Collagen Concentration in Hydrogel on the Growth and Phenotypic Expression of Rat Chondrocytes

Jin

Kim

2017

Tissue Eng Regen Med

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“…Functional hydrogels with biocompatibility have become a class of interesting biomaterials and been developed by using biocompatible polymers [1,2]. Hydrogel has been employed as a source for incorporation of bioactive molecules and their release, as well as diffusion of nutrients for cells and metabolites of entrapped cells [3], when applied in biomedical purposes, especially in tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Biocompatibility Characterizations of in Situ Chondroitin Sulfate–Gelatin Hydrogel for Tissue Engineering

Bang

Jung

Noh

2017

Tissue Eng Regen Med

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Novel hydrogel composed of both chondroitin sulfate (CS) and gelatin was developed for better cellular interaction through two step double crosslinking of N-(3-diethylpropyl)-N-ethylcarbodiimide hydrochloride (EDC) chemistries and then click chemistry. EDC chemistry was proceeded during grafting of amino acid dihydrazide (ADH) to carboxylic groups in CS and gelatin network in separate reactions, thus obtaining CS-ADH and gelatin-ADH, respectively. CS-acrylate and gelatin-TCEP was obtained through a second EDC chemistry of the unreacted free amines of CS-ADH and gelatin-ADH with acrylic acid and tri(carboxyethyl)phosphine (TCEP), respectively. In situ CS-gelatin hydrogel was obtained via click chemistry by simple mixing of aqueous solutions of both CS-acrylate and gelatin-TCEP. ATR-FTIR spectroscopy showed formation of the new chemical bonds between CS and gelatin in CS-gelatin hydrogel network. SEM demonstrated microporous structure of the hydrogel. Within serial precursor concentrations of the CS-gelatin hydrogels studied, they showed trends of the reaction rates of gelation, where the higher concentration, the quicker the gelation occurred. In vitro studies, including assessment of cell viability (live and dead assay), cytotoxicity, biocompatibility via direct contacts of the hydrogels with cells, as well as measurement of inflammatory responses, showed their excellent biocompatibility. Eventually, the test results verified a promising potency for further application of CS-gelatin hydrogel in many biomedical fields, including drug delivery and tissue engineering by mimicking extracellular matrix components of tissues such as collagen and CS in cartilage.

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“…[4]. TE with three basic elements include cells, biodegradable scaffolds, and growth factors strives to go back and reclaim the performance of the damaged tissues and organs [5,6]. The scaffold's have pivotal role in tissues regeneration, provide simulated environment similar to the extra cellular matrix (ECM) for cells migration, adhesion, and proliferation.…”

Section: Introductionmentioning

confidence: 99%

“…With its plausible biocompatibility property, PLGA are widely used in bone and cartilage TE applications. However, limitation in cell adhesion and high price are two drawbacks of PLGA as a biomaterial [6][7][8][9].Despite low cell attachment affinity of PLGA, using other materials such as bioceramics (e.g., bioactive glasses (BGs) nanoparticle) as fillers/ reinforcement components in PLGA matrix could provide a distinguished surface in order to improve cellular interaction and intensify its application in hard tissue regeneration [10][11][12]. Hyaluronic acid (Ha) is a kind of native biological materials that can be find in synovial fluid with excellent ability to support chondrocytes and mesenchymal stem cells (MSCs), noticeable biocompatibility, biodegradability, differentiated cell phenotype, well cell expansion, and little toxicity that can be used in soft and hard tissues [6 ,13].…”

Section: Introductionmentioning

confidence: 99%

“…As a cell delivery behaviour point of view, fibrin has been utilized with mesenchymal stem cells (MSCs) for soft and hard tissues (e.g., ligament, bone and cartilage). Meanwhile, because it's have weak mechanical properties and short biodegradation time, TE applications have been limited [6,16,17]. Using of fibrin to immobilize cells and to provide homogenous cells distribution in PLGA scaffolds have been displayed chondrocytes proliferation, high level of cartilage-specific proteins secretion and promotion the cartilaginous tissue formation [18].…”

Section: Introductionmentioning

confidence: 99%

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A novel nano-composite scaffold for cartilage tissue engineering

Mehdikhani-Nahrkhalaji

Tavakoli

Kharazi

et al. 2017

Scientia Iranica

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Abstract:In this study, a hybrid Poly (lactic-co-glycolic acid) (PLGA)/ Hyaluronic acid (Ha)/ Fibrin/ 45S Bioactive Glass (45SBG) nanocomposite scaffolds seeded with human Adipose-Derived Mesenchymal Stem Cells (hADMSCs) were investigated as a construct for osteoarthritis (OA), articular cartilage (AC), and subchondral bone defects therapies. The bioactivity and biodegradation of the nanocomposite scaffolds were assessed in simulated body fluid (SBF) and phosphate buffer saline (PBS) solution, respectively. Furthermore, MTT analysis was performed in order to determine attachment and viability of hADMSCs. Ultimately, results indicated that bioactivity were increased in nanocomposite scaffolds as compared to the pure PLGA scaffold. As well as, biodegradation assay exhibited that the addition of Ha, fibrin, and 45SBG nanoparticles could modify the degradation rate of PLGA. The nanocomposite scaffolds were not showed any cytotoxicity and the hADMSCs were attached on the scaffolds and proliferate properly. According to our investigation, it was concluded that using natural and synthetic polymers along with BG nanoparticles may provide a suitable construct and could show a beneficial role in AC tissue engineering and OA therapy.

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Tissue engineering of articular cartilage: From bench to bed-side

Cited by 16 publications

References 74 publications

Effects of Type I Collagen Concentration in Hydrogel on the Growth and Phenotypic Expression of Rat Chondrocytes

Effects of Type I Collagen Concentration in Hydrogel on the Growth and Phenotypic Expression of Rat Chondrocytes

Synthesis and Biocompatibility Characterizations of in Situ Chondroitin Sulfate–Gelatin Hydrogel for Tissue Engineering

A novel nano-composite scaffold for cartilage tissue engineering

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