Tissue Engineering Based on Cell Sheet Technology

Abstract

Cell sheet technology enables novel approaches to tissue engineering without the use of biodegradable scaffolds. Cell sheet technology consists of a temperature‐responsive culture dish, which enables reversible cell adhesion to and detachment from the dish surface by controllable hydrophobicity of the surface. This allows for a non‐invasive harvest of cultured cells as an intact monolayer cell sheet including deposited extra cellular matrices. The monolayer cell sheet can be transplanted to host tissues without using biodegradable scaffolds and sutures. Thick tissue constructs and patterned cell sheets using two or more kinds of cell source are also developed by means of layered cell sheets in vitro. This Progress Report summarizes temperature‐controlled cell adhesion‐detachment behavior and applications of the cell sheet technology to regeneration of cornea, periodontal ligament, bladder epithelia, oesophageal epithelia, myocardium, and liver.

The past, present and future in scaffold-based tendon treatments

“…To this end, direct cell injections were pioneered, with positive results in equine patients [44,214], even with low number of cells [215][216][217]. However, direct cell injections have failed to deliver in a consistent manner in humans due to poor cell localisation [218][219][220][221], triggering an extensive investigation into the optimal cell carrier for tendon repair [1]. The ideal carrier system should prevent cell membrane rupture during the injection process; create increased tissue integration through fast in situ self-assembly; facilitate long-term cell survival and functionality maintenance; and allow spatiotemporal release of the cargo [222][223][224][225][226][227][228][229][230][231].…”

Experimental Study of Bone Marrow Stem Cell Inducing Osteogenesis on SLA Titanium Mesh Surfac

“…Then, softer blunt latex tweezers should be used for clipping cell sheet. Then cell sheet and culture dish are completed separated [4][5][6][7][8][9][10]. Cultivation cell patch process is shown in figure 2.…”

Concise Review: Cell Therapy and Tissue Engineering for Cardiovascular Disease

“…Three-dimensional tissue can be easily fabricated by layering cell sheets detached from temperature-responsive culture surfaces (Fig. 1) [11,12]. In addition, cell sheets can be directly transplanted onto the heart surface without suture, and the cells within sheets can be effectively delivered without cell loss, because cell sheets preserve their own ECM [11,12].…”

“…1) [10]. In contrast to the scaffold-based technology, "cell sheet engineering" allows us to fabricate 3D tissues by layering cell sheets without scaffolds [11,12].…”