Tissue engineering and the road to whole organs

Vacanti, Joseph P.

doi:10.1002/bjs.7819

Cited by 21 publications

(13 citation statements)

References 63 publications

(121 reference statements)

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“…Patterning vascular architecture remains a key challenge in engineering complex, metabolically active tissues such as kidney and liver (9)(10)(11)(12)23). Previously, physical and biochemical means have been used to spatially guide the host angiogenic response in tissue engineered constructs (12-16, 24, 25).…”

Section: Discussionmentioning

confidence: 99%

Geometric control of vascular networks to enhance engineered tissue integration and function

Baranski

Chaturvedi

Stevens

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

240

233

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Tissue vascularization and integration with host circulation remains a key barrier to the translation of engineered tissues into clinically relevant therapies. Here, we used a microtissue molding approach to demonstrate that constructs containing highly aligned "cords" of endothelial cells triggered the formation of new capillaries along the length of the patterned cords. These vessels became perfused with host blood as early as 3 d post implantation and became progressively more mature through 28 d. Immunohistochemical analysis showed that the neovessels were composed of human and mouse endothelial cells and exhibited a mature phenotype, as indicated by the presence of alpha-smooth muscle actin-positive pericytes. Implantation of cords with a prescribed geometry demonstrated that they provided a template that defined the neovascular architecture in vivo. To explore the utility of this geometric control, we implanted primary rat and human hepatocyte constructs containing randomly organized endothelial networks vs. ordered cords. We found substantially enhanced hepatic survival and function in the constructs containing ordered cords following transplantation in mice. These findings demonstrate the importance of multicellular architecture in tissue integration and function, and our approach provides a unique strategy to engineer vascular architecture.tissue engineering | regenerative medicine | angiogenesis | vascular biology | liver

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Section: Discussionmentioning

confidence: 99%

Geometric control of vascular networks to enhance engineered tissue integration and function

Baranski

Chaturvedi

Stevens

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

240

233

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“…The possibility of using decellularized whole porcine hearts combined with human cells to create viable hearts for transplant is getting closer to reality. 1 Porcine hearts are similar to human hearts in size and anatomy, and the use of porcine heart valves in humans has a long history of success. 123 The development of the decellularization process in rats 12 has been successfully scaled to porcine hearts.…”

Section: Discussionmentioning

confidence: 99%

“…Great progress has been made in culturing patient-specific human cells (e.g., induced pluripotent stem cells [iPSCs]) and the promise of constructing a whole human organ from autologous human cells and decellularized porcine organ scaffolds is getting closer to reality. 1 The two steps in this process are decellularization of porcine organs while preserving the extracellular matrix (ECM) and recellularization with a human patient's cells. To fully develop this technology, there is a need to optimize decellularization processes and equipment to reliably and safely produce acellular hearts in large quantities for combination with human cells.…”

Section: Introductionmentioning

confidence: 99%

Strategies and Processes to Decellularize and Recellularize Hearts to Generate Functional Organs and Reduce the Risk of Thrombosis

Momtahan

Sukavaneshvar²,

Roeder

et al. 2015

Tissue Engineering Part B: Reviews

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Heart failure is one of the leading causes of death in the United States. Current therapies, such as heart transplants and bioartificial hearts, are helpful, but not optimal. Decellularization of porcine whole hearts followed by recellularization with patient-specific human cells may provide the ultimate solution for patients with heart failure. Great progress has been made in the development of efficient processes for decellularization, and the design of automated bioreactors. Challenges remain in selecting and culturing cells, growing the cells on the decellularized scaffolds without contamination, characterizing the regenerated organs, and preventing thrombosis. Various strategies have been proposed to prevent thrombosis of blood-contacting devices, including reendothelization and the creation of nonfouling surfaces using surface modification technologies. This review discusses the progress and remaining challenges involved with recellularizing whole hearts, focusing on the prevention of thrombosis.

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“…Although there are many challenges, there is a strong base of public support for these modern RM opportunities to create curative therapeutics for a brighter future in disease management [99][100][101][102].…”

Section: The Future and Challengesmentioning

confidence: 99%

Advent and Maturation of Regenerative Medicine

Mhashilkar

Atala²

2013

Pharmaceutical Sciences Encyclopedia

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The goals for regenerative medicine (RM) are focused on developing innovative tools in modern medicine. Recent progress has led to the development and clinical implementation of newer technologies, ranging from diagnostics to therapeutics for a wide variety of metabolic, degenerative, and infectious diseases.These include specific applications toward age‐ and genetic‐related human and animal dysfunctions and disorders. The focus of this review is to present, in a nutshell, the current status of adult stem cell therapy (CT) and tissue engineering (TE), including the challenges faced, progresses attained, and an optimistic view of the future. We will also provide our views on how the next 20+ years will potentially affect these RM‐driven innovations and how they are applied toward a variety of disease treatments, cures, and diagnostics.

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Tissue engineering and the road to whole organs

Abstract: A glimpse into the future of surgery

Cited by 21 publications

References 63 publications

Geometric control of vascular networks to enhance engineered tissue integration and function

Geometric control of vascular networks to enhance engineered tissue integration and function

Strategies and Processes to Decellularize and Recellularize Hearts to Generate Functional Organs and Reduce the Risk of Thrombosis

Advent and Maturation of Regenerative Medicine

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