Tissue engineered poly(caprolactone)‐chitosan‐poly(vinyl alcohol) nanofibrous scaffolds for burn and cutting wound healing

Abstract

Natural-synthetic blend nanofibres have recently attracted more interest because of the ability of achieving desirable properties. Poly(ε-caprolactone) (PCL)-chitosan (Cs)-poly(vinyl alcohol) (PVA) blend nanofibrous scaffolds were electrospun in 2:1:1.33 mass ratio of PCL:Cs:PVA. The presence of PCL in the blend leads to improvement in web hydrophobicity and helped the web to retain its integrity in aqueous media. The scaffolds were used in two forms of acellular and with mesenchymal stem cells. They were applied on burn (n = 12) and excisional cutting (n = 12) wounds on dorsum skin of rats. Macroscopic investigations were carried out to measure the wounds areas. It was found that the area of wounds that were treated with cell-seeded nanofibrous scaffolds were smaller compared to other samples. Pathological results showed much better healing performance for cell-seeded scaffolds followed by acellular scaffolds compared with control samples. All these results indicate that PCL:Cs:PVA nanofibrous web would be a proper material for burn and cutting wound healing.

Ionically Crosslinked Thermoresponsive Chitosan Hydrogels formed In Situ: A Conceptual Basis for Deeper Understanding

“…Modifications of chitosan chemistry or solutions can improve the interaction with bioactive elements and improve cell attachment . The addition of β‐glycerolphosphate (β‐GP) increased the pH of chitosan solutions to a neutral value without phase separation .…”

Polymeric membranes modulate human keratinocyte differentiation in specific epidermal layers

“…gelatin, collagen) in order to tune the desired mechanical properties and biodegradation rate for the realization of epidermal and bi-layer skin substitutes [17,18]. CHT-PCL blends have been tested for skin applications, as a potential wound dressing and to heal burns and cuts [19][20][21].…”

Preparation, Characterization and Evaluation of Antifungal Efficacy of Chitosan/Soy Phosphatidylcholine Topical Films Containing Griseofulvin

“…Due to the hydrophilic nature of chitosan, films composed of chitosan alone showed lack of stability, chemical cross linking with agents such as glutaraldehyde could overcome this, but they have been found to be toxic and the films are often non-biodegradable (3). Blending chitosan with other materials to produce stable films by physical cross-linking achieved via electrostatic and/or hydrogen bonding interaction has been investigated widely (3)(4)(5)(6)(7)(8)(9)(10).…”