Tissue-engineered cardiovascular grafts and novel applications of tissue engineering by self-assembly (TESA™)

Mount, Cristin; Dusserrre, N.; McAllister, Todd; L’Heureux, Nicolas

doi:10.1533/9780857096715.3.410

Cited by 4 publications

(5 citation statements)

References 231 publications

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“…However, in addition to a manufacturing cost of greater than $15,000 per graft, another criticism of this approach is that it will not help patients who need rapid intervention because it can take up to 9 months to manufacture [42].…”

Section: Application Of Cell Biology To Tevgmentioning

confidence: 99%

The Evolution of Tissue Engineered Vascular Graft Technologies: From Preclinical Trials to Advancing Patient Care

et al. 2019

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Currently available synthetic grafts have contributed to improved outcomes in cardiovascular surgery. However, the implementation of these graft materials at small diameters have demonstrated poor patency, inhibiting their use for coronary artery bypass surgery in adults. Additionally, when applied to a pediatric patient population, they are handicapped by their lack of growth ability. Tissue engineered alternatives could possibly address these limitations by producing biocompatible implants with the ability to repair, remodel, grow, and regenerate. A tissue engineered vascular graft (TEVG) generally consists of a scaffold, seeded cells, and the appropriate environmental cues (i.e., growth factors, physical stimulation) to induce tissue formation. This review critically appraises current state-of-the-art techniques for vascular graft production. We additionally examine current graft shortcomings and future prospects, as they relate to cardiovascular surgery, from two major clinical trials.

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Section: Application Of Cell Biology To Tevgmentioning

confidence: 99%

The Evolution of Tissue Engineered Vascular Graft Technologies: From Preclinical Trials to Advancing Patient Care

et al. 2019

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“…In related vascular applications, human cryopreserved allografts were implanted as hemodialysis access grafts and seem less prone to fibrosis, calcification, and degeneration as compared to their xenograft counterparts. Particularly CryoLife (Synergraft) implanted cryopreserved cadaver femoral allografts decellularized in an antigen-reduced manner and showed comparative results to PTFE grafts (McAllisteret al 2009; Mount et al 2014). CryoLife also produced SynerGraft aortic valve conduit from human valves that were decellularized before cryopreservation.…”

Section: Decellularized Xenografts and Allograftsmentioning

confidence: 99%

“…This strategy allows the cells themselves to produce the scaffold, resulting in a completely biological TEVG with impressive mechanical properties. L’Heureux pioneered this strategy using autologous fibroblasts grown on flat culture plates in the presence of elevated ascorbic acid (to increase collagen synthesis), which results in fibroblasts sheets embedded in fibrillar collagenous ECM (Mount et al 2014). The sheets are then wrapped around a tubular mandrel, allowing the individual plies to fuse together, creating tubular structures.…”

Section: Tissue-engineered Vascular Graftsmentioning

confidence: 99%

Historical Perspective and Future Direction of Blood Vessel Developments

Dimitrievska

Niklason

2017

Cold Spring Harb Perspect Med

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Over the past 40 years, remarkable advances have been made in our understanding of successful blood vessel regeneration, starting with the failures of early tissue-engineered vascular grafts designed using isolated components or molecules, such as collagen gels. The vascular tissue engineers are today better educated and have steered ongoing research developments toward clinical developments of more complete vascular grafts that replicate the multitude of specialized arterial aspects required for function.

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“…Small diameter blood vessels followed by upstream vasoconstriction is often prone to secondary stenosis [ 1 ]. Hence, regenerated blood vessels will be an appropriate solution for replacing these small diameter vessels (which were affected with secondary stenosis).…”

Section: Introductionmentioning

confidence: 99%

Numerical and Experimental Analysis of Shear Stress Influence on Cellular Viability in Serpentine Vascular Channels

et al. 2022

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3D bioprinting has emerged as a tool for developing in vitro tissue models for studying disease progression and drug development. The objective of the current study was to evaluate the influence of flow driven shear stress on the viability of cultured cells inside the luminal wall of a serpentine network. Fluid–structure interaction was modeled using COMSOL Multiphysics for representing the elasticity of the serpentine wall. Experimental analysis of the serpentine model was performed on the basis of a desirable inlet flow boundary condition for which the most homogeneously distributed wall shear stress had been obtained from numerical study. A blend of Gelatin-methacryloyl (GelMA) and PEGDA200 PhotoInk was used as a bioink for printing the serpentine network, while facilitating cell growth within the pores of the gelatin substrate. Human umbilical vein endothelial cells were seeded into the channels of the network to simulate the blood vessels. A Live-Dead assay was performed over a period of 14 days to observe the cellular viability in the printed vascular channels. It was observed that cell viability increases when the seeded cells were exposed to the evenly distributed shear stresses at an input flow rate of 4.62 mm/min of the culture media, similar to that predicted in the numerical model with the same inlet boundary condition. It leads to recruitment of a large number of focal adhesion point nodes on cellular membrane, emphasizing the influence of such phenomena on promoting cellular morphologies.

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Tissue-engineered cardiovascular grafts and novel applications of tissue engineering by self-assembly (TESA™)

Cited by 4 publications

References 231 publications

The Evolution of Tissue Engineered Vascular Graft Technologies: From Preclinical Trials to Advancing Patient Care

The Evolution of Tissue Engineered Vascular Graft Technologies: From Preclinical Trials to Advancing Patient Care

Historical Perspective and Future Direction of Blood Vessel Developments

Numerical and Experimental Analysis of Shear Stress Influence on Cellular Viability in Serpentine Vascular Channels

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