Tissue-engineered bone formation using periosteal-derived cells and polydioxanone/pluronic F127 scaffold with pre-seeded adipose tissue-derived CD146 positive endothelial-like cells

Lee, Jinho; Kim, Jin-Hyun; Oh, Se-Heang; Kim, Sang-June; Hah, Young‐Sool; Park, Bong-Wook; Kim, Deok Ryong; Rho, Gyu‐Jin; Maeng, Geun-Ho; Jeon, Ryoung-Hoon; Lee, Hee-Chun; Kim, Jongryoul; Kim, Gyoo-Cheon; Kim, Uk-Kyu; Byun, June‐Ho

doi:10.1016/j.biomaterials.2011.03.081

Cited by 32 publications

(31 citation statements)

References 26 publications

(24 reference statements)

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“…The balance between porosity and mechanical strength is the key factor in the fabrication of scaffolds. 102 Several methods have been used for porous scaffold fabrication, including molding, 57 foaming, 21 leaching, 64 template-casting, 45 machining, 27 layer-by-layer assembly (LBL), 71 lithographic techniques, 67 and additive manufacturing. 107 Metals, such as titanium, 18 tantalum, 118 and magnesium, 109 ceramics such as calcium phosphate, 113 and polymers ranging from polyesters, 79 polyurethanes, 15 and polycarbonates, 65 to more specialized chemistries, such as polyanhydrides, 99 polyphosphazenes, 86 and polypropylene fumarates, 23 have provided a basic array of materials that can be used for scaffold fabrication.…”

Section: Tissue Engineering Approaches For Vascularized Bone Repairmentioning

confidence: 99%

Vascularization in Bone Tissue Engineering Constructs

et al. 2015

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Vascularization of large bone grafts is one of the main challenges of bone tissue engineering (BTE), and has held back the clinical translation of engineered bone constructs for two decades so far. The ultimate goal of vascularized BTE constructs is to provide a bone environment rich in functional vascular networks to achieve efficient osseointegration and accelerate restoration of function after implantation. To attain both structural and vascular integration of the grafts, a large number of biomaterials, cells, and biological cues have been evaluated. This review will present biological considerations for bone function restoration, contemporary approaches for clinical salvage of large bone defects and their limitations, state-of-the-art research on the development of vascularized bone constructs, and perspectives on evaluating and implementing novel BTE grafts in clinical practice. Success will depend on achieving full graft integration at multiple hierarchical levels, both between the individual graft components as well as between the implanted constructs and their surrounding host tissues. The paradigm of vascularized tissue constructs could not only revolutionize the progress of bone tissue engineering, but could also be readily applied to other fields in regenerative medicine for the development of new innovative vascularized tissue designs.

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Section: Tissue Engineering Approaches For Vascularized Bone Repairmentioning

confidence: 99%

Vascularization in Bone Tissue Engineering Constructs

et al. 2015

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“…47,[90][91][92][93][94][95][96][97][98][99][100] PDCs have been used to construct TEP with and without the use of scaffolds. Rat femoral shaft defects were successfully healed using TEP constructed from PDCs seeded on a polyglycolic acid scaffold.…”

Section: Bridging the Cell-tissue Level: Tissue Engineered Periosteummentioning

confidence: 99%

Elucidating Multiscale Periosteal Mechanobiology: A Key to Unlocking the Smart Properties and Regenerative Capacity of the Periosteum?

Evans

Chang

Tate

2013

Tissue Engineering Part B: Reviews

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“…Although the exact mechanisms in which F68 increases neuro-and myogenic differentiation of human stem cells need to be explored, one possible explanation for the effects of pluronics might be the stabilization of the cell membrane during differentiation resulting in effective lineage transformation as cell membrane tension and surface area might direct cell fate and differentiation (McBeath et al 2004;Titushkin and Cho 2006). F127 has been used for several medical approaches including drug and gene delivery (Kedar et al 2010), scaffold fabrication for tissue engineering practices (Lee et al 2011), targeted therapy and diagnosis (Lin et al 2009). Despite the multiple advantages of F127 in biomedical applications, it did not enhance in vitro myocyte and neuronal differentiation of hTGSCs in the current study.…”

Section: Discussionmentioning

confidence: 99%

Myogenic and neurogenic differentiation of human tooth germ stem cells (hTGSCs) are regulated by pluronic block copolymers

et al. 2015

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Stem cells with high proliferation, selfrenewal and differentiation capacities are promising for tissue engineering approaches. Among stem cells, human tooth germ stem cells (hTGSCs) having mesenchymal stem cell characteristics are highly proliferative and able to differentiate into several cell lineages. Researchers have recently focused on transplanting stem cells with bioconductive and/or bioinductive materials that can provide cell commitment to the desired cell lineages. In the present study, effects of pluronic block copolymers (F68, F127 and P85) on in vitro myo-and neurogenic differentiation of human tooth germ stem cells (hTGSCs) were investigated. As P85 was found to exert considerable toxicity to hTGSCs even at low concentrations, it was not evaluated for further differentiation experiments. Immunocytochemical analysis, gene and protein expression studies revealed that while F68 treatment increased lineage-specific gene expression in both myo-and neuro-genically differentiated cells, F127 did not result in any remarkable difference compared to cells treated with differentiation medium. Subsequent studies are required to explore the exact mechanisms of how F68 increases the myogenic and neurogenic differentiation of hTGSCs. The present work indicates that pluronic F68 might be used in functional skeletal and neural tissue engineering applications.

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Tissue-engineered bone formation using periosteal-derived cells and polydioxanone/pluronic F127 scaffold with pre-seeded adipose tissue-derived CD146 positive endothelial-like cells

Cited by 32 publications

References 26 publications

Vascularization in Bone Tissue Engineering Constructs

Vascularization in Bone Tissue Engineering Constructs

Elucidating Multiscale Periosteal Mechanobiology: A Key to Unlocking the Smart Properties and Regenerative Capacity of the Periosteum?

Myogenic and neurogenic differentiation of human tooth germ stem cells (hTGSCs) are regulated by pluronic block copolymers

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